Recombinant Human Follistatin-like 5/FSTL5 Protein, CF

R&D Systems | Catalog # 9066-FN

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Follistatin-like 5/FSTL5 Protein (9066-FN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

AAH36502

Applications

Bioactivity
View all Follistatin-like 5/FSTL5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Follistatin-like 5/FSTL5 protein
Arg21-Ala847, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg21

Predicted Molecular Mass

94 kDa

SDS-PAGE

86-102 kDa, reducing conditions

Activity

Measured by its ability to inhibit Hep3B human hepatocellular carcinoma cell survival.
The ED50 for this effect is 3-12 μg/mL.

Scientific Data Images for Recombinant Human Follistatin-like 5/FSTL5 Protein, CF

Recombinant Human Follistatin-like 5/FSTL5 Protein Bioactivity

Recombinant Human Follistatin-like 5/FSTL5 Protein Bioactivity

Recombinant Human Follistatin-like 5/FSTL5 (Catalog # 9066-FN) inhibits Hep3B human hepatocellular carcinoma cell survival. The ED50 for this effect is 3-12 μg/mL.

Formulation, Preparation, and Storage

9066-FN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Follistatin-like 5/FSTL5

Follistatin-like 5 (FSTL5) is a secreted 94 kDa (predicted) member of the SPARC protein family (1). In its N-terminal half, FSTL5 contains a Kazal-like domain,
two EF-hand domains, and two Ig-like domains. Mature human FSTL5 shares 91% amino acid sequence identity with mouse and rat FSTL5. Alternative splicing generates an isoform with a 10 residue deletion following the second Ig-like domain. FSTL5 is expressed in the cartilage and tendon of developing digits and the shafts of long bones (2). In the brain it is expressed in the olfactory bulb, CA3 area of the hippocampus, and granule cell layer of the cerebellum (3). FSTL5 inhibits the proliferation and promotes the apoptosis of hepatocellular carcinoma cells (4). Its expression in medulloblastoma and down-regulation in hepatocellular carcinoma are associated with poor prognosis (4, 5).

References

  1. Bradshaw, A.D. (2012) Int. J. Biochem. Cell Biol. 44:480.
  2. Lorda-Diez, C.I. et al. (2013) PLoS One 8:e60423.
  3. Masuda, T. et al. (2014) Congenit. Anom. (Kyoto) 54:63.
  4. Zhang, D. et al. (2015) Int. J. Clin. Exp. Pathol. 8:3386.
  5. Remke, M. et al. (2011) J. Clin. Oncol. 29:3852.

Alternate Names

Follistatin like 5, FSL5, FSTL5

Entrez Gene IDs

56884 (Human); 213262 (Mouse); 365823 (Rat)

Gene Symbol

FSTL5

UniProt

AAH36502

Additional Follistatin-like 5/FSTL5 Products

Product Documents for Recombinant Human Follistatin-like 5/FSTL5 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Follistatin-like 5/FSTL5 Protein, CF

For research use only

Related Research Areas

Activin Family

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