Recombinant Human FOLR1 Protein, CF

R&D Systems | Catalog # 5646-FR

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human FOLR1 Protein (5646-FR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P15328

Applications

Binding Activity
View all FOLR1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human FOLR1 protein
Arg25-Met233, with a C-terminal 6x-His tag
        

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg25

Predicted Molecular Mass

25.4 kDa

SDS-PAGE

30-40 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Folic Acid-Bovine Serum Albumin is coated at 5 μg/mL (100 μL/well), the concentration of rhFOLR-1 that produces 50% of the optimal binding response is found to be approximately 0.4 -1.6 nM.

Reviewed Applications

Read 3 reviews rated 5 using 5646-FR in the following applications:

Formulation, Preparation, and Storage

5646-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FOLR1

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3 - 6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).

References

  1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  2. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
  4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
  5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
  6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
  7. Ross, J.F. et al. (1994) Cancer 73:2432.
  8. Parker, N. et al. (2005) Anal. Biochem. 338:284.
  9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
  11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.

Long Name

Folate Receptor 1

Alternate Names

FBP, Folbp1, FR-alpha, MOv18

Entrez Gene IDs

2348 (Human); 14275 (Mouse); 171049 (Rat)

Gene Symbol

FOLR1

UniProt

P15328 (Human)

Additional FOLR1 Products

Product Documents for Recombinant Human FOLR1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human FOLR1 Protein, CF

For research use only

Related Research Areas

Cancer Biomarkers
Neuronal Markers

Citations for Recombinant Human FOLR1 Protein, CF

Customer Reviews for Recombinant Human FOLR1 Protein, CF (3)

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  • Recombinant Human FOLR1 Protein, CF
    Name: Jenna Nguyen
    Application: Cell Adhesion
    Verified Customer | Posted 10/24/2018
  • Recombinant Human FOLR1 Protein, CF
    Name: Jenna Nguyen
    Application: Cell migration/motility
    Verified Customer | Posted 10/12/2018
  • Recombinant Human FOLR1 Protein, CF
    Name: Jenna Nguyen
    Application: Immunoassay Standard
    Verified Customer | Posted 08/29/2018

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