Recombinant Human FOLR1 Protein, CF

Catalog # Availability Size / Price Qty
5646-FR-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (3)

Recombinant Human FOLR1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Folic Acid-Bovine Serum Albumin is coated at 5 μg/mL (100 μL/well), the concentration of rhFOLR-1 that produces 50% of the optimal binding response is found to be approximately 0.4 -1.6 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived human FOLR1 protein
Arg25-Met233, with a C-terminal 6x-His tag
        
Accession #
N-terminal Sequence
Analysis
Arg25
Predicted Molecular Mass
25.4 kDa
SDS-PAGE
30-40 kDa, reducing conditions

Complete Your Experiment

Product Datasheets

You must select a language.

x

5646-FR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5646-FR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: FOLR1

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3 - 6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).

References
  1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  2. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
  4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
  5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
  6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
  7. Ross, J.F. et al. (1994) Cancer 73:2432.
  8. Parker, N. et al. (2005) Anal. Biochem. 338:284.
  9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
  11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.
Long Name
Folate Receptor 1
Entrez Gene IDs
2348 (Human); 14275 (Mouse); 171049 (Rat)
Alternate Names
Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; FOLR; FOLR1; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18

Citations for Recombinant Human FOLR1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays
    Authors: J Song, LJ Sokoll, DW Chan, Z Zhang
    Biomedicines, 2021;9(12):.
    Species: Human
    Sample Types: Protein
    Applications: Reference Standard
  2. Evaluation of Antigen-Conjugated Fluorescent Beads to Identify Antigen-Specific B Cells
    Authors: I Correa, KM Ilieva, S Crescioli, S Lombardi, M Figini, A Cheung, JF Spicer, ANJ Tutt, FO Nestle, P Karagianni, KE Lacy, SN Karagianni
    Front Immunol, 2018;9(0):493.
    Species: Human
    Sample Types: Protein
    Applications: Protein Array
  3. Conditions associated with circulating tumor-associated folate receptor 1 protein in healthy men and women.
    Authors: Kelemen L, Brenton J, Parkinson C, C Whitaker H, Piskorz A, Csizmadi I, Robson P
    PLoS ONE, 2014;9(5):e96542.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  4. Harnessing engineered antibodies of the IgE class to combat malignancy: initial assessment of FcvarepsilonRI-mediated basophil activation by a tumour-specific IgE antibody to evaluate the risk of type I hypersensitivity.
    Authors: Rudman SM, Josephs DH, Cambrook H, Karagiannis P, Gilbert AE, Dodev T, Hunt J, Koers A, Montes A, Taams L, Canevari S, Figini M, Blower PJ, Beavil AJ, Nicodemus CF, Corrigan C, Kaye SB, Nestle FO, Gould HJ, Spicer JF, Karagiannis SN
    Clin. Exp. Allergy, 2011;41(10):1400-1413.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Capture)

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human FOLR1 Protein, CF

Average Rating: 5 (Based on 3 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Recombinant Human FOLR1 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Recombinant Human FOLR1 Protein, CF
By Jenna Nguyen on 10/24/2018
Application: Cell Adhesion

Recombinant Human FOLR1 Protein, CF
By Jenna Nguyen on 10/12/2018
Application: Cell migration/motility

Recombinant Human FOLR1 Protein, CF
By Jenna Nguyen on 08/29/2018
Application: Immunoassay Standard