Recombinant Human GASP-2/WFIKKN Protein

Carrier Free

Catalog # Availability Size / Price Qty
2136-GS-025/CF

With Carrier

Catalog # Availability Size / Price Qty
2136-GS-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (1)
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Recombinant Human GASP-2/WFIKKN Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.25-1.25 µg/mL, in the presence of 40 ng/mL of rmGDF-8.
Source
Mouse myeloma cell line, NS0-derived human GASP-2/WFIKKN protein
Ala20-Asp548, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala20
Predicted Molecular Mass
57.5 kDa
SDS-PAGE
65-72 kDa, reducing conditions

Product Datasheets

2136-GS (with carrier)

2136-GS/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2136-GS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2136-GS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: GASP-2/WFIKKN

Growth and differentiation factor-associated serum protein-2 (GASP-2) cDNA encodes a 548 amino acid protein that contains a 19 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Based on the order of these conserved domains, GASP-2 is also known as WFIKKN (1). Another related protein which contains the same domain structure is called WFIKKNRP (WFIKKN-related protein), or GASP-1 (2, 3). WAP, follistatin, Kunitz and netrin domains are all implicated in protease inhibition, and the GASP proteins may be multivalent protease inhibitors (1, 4). Tests at R&D systems have measured the ability of GASP-2 to inhibit trypsin cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (R&D Systems Catalog # ES002). The IC50 value was approximately 10 nM, as measured in a reaction mixture containing 1.0 nM trypsin, 10 µM ES002, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.

GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-2 is abundant in the lung, skeletal muscle and liver while GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney (3). In the adult, GASP-2 is expressed primarily in the pancreas, liver, and thymus while GASP-1 is in the ovary, testis, and brain (3). Further characterization shows that GASP-1 inhibits myostatin (GDF-8) and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (2). Although, this kind of activity has not been reported for GASP-2, tests at R&D Systems have determined that GASP-2 shows similar inhibitory activity towards myostatin as GASP-1. By amino acid sequence, human GASP-2 is 55% identical to human GASP-1.

References
  1. Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3705.
  2. Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
  3. Trexler, M. et al. (2002) Biol. Chem. 383:223.
  4. Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
Long Name
Growth and Differentiation Factor-associated Serum Protein-2
Entrez Gene IDs
117166 (Human); 215001 (Mouse)
Alternate Names
C16orf12; Chromosome 16 Open Reading Frame 12; GASP2; GASP-2; Growth And Differentiation Factor-Associated Serum Protein 2; hGASP-2; RJD2; WAP Four-Disulfide Core Domain 20A; WAP, Follistatin/Kazal, Immunoglobulin, Kunitz And Netrin Domain Containing 1; WAP, FS, Ig, KU, And NTR-Containing Protein; WFDC20A; WFIKKN; WFIKKN1

Citation for Recombinant Human GASP-2/WFIKKN Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Structural basis for potency differences between GDF8 and GDF11
    Authors: RG Walker, M Czepnik, EJ Goebel, JC McCoy, A Vujic, M Cho, J Oh, S Aykul, KL Walton, G Schang, DJ Bernard, AP Hinck, CA Harrison, E Martinez-H, AJ Wagers, RT Lee, TB Thompson
    BMC Biol, 2017;15(1):19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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