Recombinant Human Guanylyl Cyclase C His-tag Protein, CF

Catalog # Availability Size / Price Qty
2157-GC-050
Recombinant Human Guanylyl Cyclase C His-tag Protein, CF SDS-PAGE
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Recombinant Human Guanylyl Cyclase C His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 2.5 μg/mL of protein is able to significantly inhibit neurite outgrowth.
Source
Human embryonic kidney cell, HEK293-derived human Guanylyl Cyclase C/GUCY2C protein
Ser21-Gln430, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser21
Predicted Molecular Mass
47 kDa
SDS-PAGE
73-81 kDa, reducing conditions

Product Datasheets

2157-GC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE Recombinant Human Guanylyl Cyclase C His-tag Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human Guanylyl Cyclase C/GUCY2C was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 73-81 kDa.

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Background: Guanylyl Cyclase C/GUCY2C

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP (1, 2). GUCY2C contains a 23 amino acid (aa) signal sequence, a 407 aa extracellular region (ECD), a 24 aa transmembrane (TM) segment and a 619 aa cytoplasmic domain (3). The ECD of human GUCY2C shares 71% and 72% aa identity with mouse and rat GUCY2C, respectively (4). GUCY2C was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (5). Endogenous ligands of GUCY2C include guanylin and uroguanylin (6). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis (2, 6). GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (2, 7). CAR-T cell therapy utilizing GUCY2C to treat metastatic colorectal cancer is currently being explored (8).

References
  1. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
  2. Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
  3. de Sauvage, F. J. et al. (1991) J. Biol. Chem. 266:17912.
  4. Singh S. et al. (1991) Biochem. Biophys. Res. Comm. 179:1455.
  5. Lucas K. et al. (2000) Pharmacol Rev. 52:375.
  6. Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
  7. Gong, R. et al. (2011) Science. 333:1642.
  8. Magee, MS. et al. (2018) Cancer Immuno Res. 6:509.
Entrez Gene IDs
2984 (Human); 14917 (Mouse); 25711 (Rat)
Alternate Names
DIAR6; EC 4.6.1; GCC; GC-C; guanylate cyclase 2C (heat stable enterotoxin receptor); Guanylate Cyclase C; Guanylyl Cyclase C; GUC2C; GUC2CEC 4.6.1.2; GUCY2C; heat-stable enterotoxin receptor; hSTAR; Intestinal guanylate cyclase; MUCIL; STA Receptor; StAR; STARSTA receptor

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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