Recombinant Human HA-GATE-16 AMC Protein, CF
Recombinant Human HA-GATE-16 AMC Protein, CF Summary
Product Specifications
Contains an N-terminal HA (YPYDVPDYA) tag
14 kDa
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UL-445
| Formulation | X mg/ml (X µM) in 50 mM MOPS pH 6.5, 100 mM NaCl |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: GATE-16
Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the Autophagy-related 8 (ATG8) family of proteins (1). GATE-16 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast ATG8. ATG8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GATE-16 is best known for its role in autophagy (2,3). GATE-16 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).
This is a fluorogenic substrate for ATG8-specific C-terminal hydrolases such as ATG4B (Catalog # E-400) based on the C-terminal derivatization of ATG8 with 7-amido-4-methylcoumarin (AMC). ATG8-AMC is useful for studying such enzyme activities when detection sensitivity or continuous monitoring of activity is essential. NOTE: this protein has an N-terminal HA tag.
- Shpilka, T. et al. (2011) Genome Biol. 12:226.
- Wang, H. et al. (1999) Nature 397:69.
- Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
- Weidberg, H. et al. (2010) EMBO J. 29:1792.
- Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.
Citation for Recombinant Human HA-GATE-16 AMC Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Cholesterol impairs autophagy-mediated clearance of amyloid beta while promoting its secretion
Authors: E Barbero-Ca, V Roca-Aguje, I Bartolessi, C de Dios, JC Fernández-, M Marí, A Morales, T Hartmann, A Colell
Autophagy, 2018-06-04;0(0):1-26.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Bioassay
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