Recombinant Human HA-Ubiquitin Protein, CF

R&D Systems | Catalog # U-110

R&D Systems
Discontinued Product
U-110 has been discontinued. View all Ubiquitin products.

Key Product Details

Source

E. coli

Accession Number

P0CG47.1

Applications

Enzyme Activity
View all Ubiquitin Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Ubiquitin protein
Met1 - Gly76 with N-terminal HA (YPYDVPDYA) tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

9.8 kDa

Activity

Recombinant Human HA-Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human HA-Ubiquitin concentration of 5-50 μM.

Reviewed Applications

Read 2 reviews rated 4.5 using U-110 in the following applications:

Formulation, Preparation, and Storage

U-110
Formulation Supplied as a solution in HEPES.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).


References

  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
  8. Coux O., et al. (1996) Ann. Rev. Biochem. 65: 801-847.
  9. Ellison M.J and Hochstrasser M. (1991) J. Biol. Chem. 266: 21150-21157.
  10. Finley D., et al. (1994) Mol. Cell. Biol. 14: 5501-5509.
  11. Fritze C.E. and Anderson T.R. (2000) Meth. Enz. 327: 3-16.
  12. Terpe K (2003) Appl. Microbiol. Biotech. 60: 523-533.
  13. Treier M., et al. (1994) Cell. 78: 787-798.
  14. Wilson I.A., et al. (1984) Cell 37:767-778.

Alternate Names

UBB

Entrez Gene IDs

7314 (Human); 298693 (Rat)

Gene Symbol

UBB

UniProt

P0CG47.1

Additional Ubiquitin Products

Product Documents for Recombinant Human HA-Ubiquitin Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human HA-Ubiquitin Protein, CF

For research use only

Citations for Recombinant Human HA-Ubiquitin Protein, CF

Customer Reviews for Recombinant Human HA-Ubiquitin Protein, CF (2)

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  • Name: valentina rossio
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 12/05/2018
  • Recombinant Human HA-Ubiquitin Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/30/2017

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