Recombinant Human HECT domain-containing 2 Protein, CF

R&D Systems | Catalog # E3-302

R&D Systems
Discontinued Product
E3-302 has been discontinued. View all HECTD2 products.

Key Product Details

Source

E. coli

Accession Number

Q5U5R9

Applications

Bioactivity
View all HECTD2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human HECTD2 protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

88 kDa

Activity

Typical enzyme concentration to support conjugation in vitro is 100-500 nM depending on experimental conditions.

Formulation, Preparation, and Storage

E3-302
Formulation X mg/ml (X μM) in 50 mM HEPES, pH 7.0, 100 mM NaCl, 5% (v/v) Glycerol, 5 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 6 months, -70 °C under sterile conditions after opening.

Background: HECTD2

HECTD2 (aka "HECT domain-containing protein 2" or "HECT-type E3 ubiquitin transferase HECTD2") is a 776 amino acid member of the HECT (Homologous to E6AP Carboxyl Terminus) domain family of Ubiquitin ligases.  With a predicted weight of 88 kDa, human HECTD2 shares 89% identity and 94% similarity with the mouse orthologue.  In innate immunity HECTD2 exerts a pro-inflammatory effect by contributing to the ubiquitylation and degradation of PIAS1, a multifunctional and potent anti-inflammatory protein that negatively regulates several key inflammatory pathways.  Small molecule inhibitors of HECTD2 were found to attenuate lipopolysaccharide (LPS)-induced lung inflammation.  HECTD2 has also been shown to ubiquitylate BoNT/A, a catalytic light chain of botulinum neurotoxin.  Ubiquitylation can lead to the proteasomal degradation of the light chain, but it's rescued from destruction by the dominant effect of a deubiquitinating enzyme, VCIP135/VCPIP1.

References

  1. Coon T.A. et al.  (2015)  Sci. Transl. Med.  doi:  10.1126/scitranslmed.aab3881
  2. Tsai Y.C. et al.  (2017) Proc. Natl. Acad. Sci.  114:  E5158-E5166

Long Name

HECT Domain E3 Ubiquitin Protein Ligase 2

Alternate Names

EC2.3.2.26

Entrez Gene IDs

143279 (Human); 226098 (Mouse); 309514 (Rat)

Gene Symbol

HECTD2

UniProt

Q5U5R9

Additional HECTD2 Products

Product Documents for Recombinant Human HECT domain-containing 2 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human HECT domain-containing 2 Protein, CF

For research use only

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