Recombinant Human HECT domain-containing 2 Protein, CF

Catalog # Availability Size / Price Qty
E3-302-025
Product Details
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Recombinant Human HECT domain-containing 2 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical enzyme concentration to support conjugation in vitro is 100-500 nM depending on experimental conditions.
Source
E. coli-derived human HECTD2 protein
Accession #
Predicted Molecular Mass
88 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-302

Formulation X mg/ml (X μM) in 50 mM HEPES, pH 7.0, 100 mM NaCl, 5% (v/v) Glycerol, 5 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -70 °C as supplied.
  • 6 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HECTD2

HECTD2 (aka "HECT domain-containing protein 2" or "HECT-type E3 ubiquitin transferase HECTD2") is a 776 amino acid member of the HECT (Homologous to E6AP Carboxyl Terminus) domain family of Ubiquitin ligases.  With a predicted weight of 88 kDa, human HECTD2 shares 89% identity and 94% similarity with the mouse orthologue.  In innate immunity HECTD2 exerts a pro-inflammatory effect by contributing to the ubiquitylation and degradation of PIAS1, a multifunctional and potent anti-inflammatory protein that negatively regulates several key inflammatory pathways.  Small molecule inhibitors of HECTD2 were found to attenuate lipopolysaccharide (LPS)-induced lung inflammation.  HECTD2 has also been shown to ubiquitylate BoNT/A, a catalytic light chain of botulinum neurotoxin.  Ubiquitylation can lead to the proteasomal degradation of the light chain, but it's rescued from destruction by the dominant effect of a deubiquitinating enzyme, VCIP135/VCPIP1.

References
  1. Coon T.A. et al.  (2015)  Sci. Transl. Med.  doi:  10.1126/scitranslmed.aab3881
  2. Tsai Y.C. et al.  (2017) Proc. Natl. Acad. Sci.  114:  E5158-E5166
Long Name
HECT Domain E3 Ubiquitin Protein Ligase 2
Entrez Gene IDs
143279 (Human); 226098 (Mouse); 309514 (Rat)
Alternate Names
EC 6.3.2.-; EC2.3.2.26; FLJ16050; FLJ37306; HECT domain containing 2; HECT domain-containing protein 2; HECTD2; probable E3 ubiquitin-protein ligase HECTD2

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