Recombinant Human His10-cIAP-2/HIAP-1 Protein, CF

Name: Recombinant Human His10-cIAP-2/HIAP-1 Protein, CF
Brand: R&D Systems
SKU: E3-285
Price: 211 USD

Catalog #: E3-285 Datasheet / COA / SDS

Discontinued Product

E3-285 has been discontinued.

View all cIAP-2/HIAP-1 products.

R&D Systems Recombinant Proteins and Enzymes

1 Image

All cIAP-2/HIAP-1 Proteins and Enzymes

Product Details

FAQs

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His10-cIAP-2/HIAP-1 Protein, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Recombinant Human cIAP-2/HIAP-1 is a RING finger Ubiquitin ligase (E3) that functions downstream of a Ubiquitinactivating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial cIAP-2 concentration of 0.2-1 μM.

Source

E. coli-derived human cIAP-2/HIAP-1 protein
Contains an N-terminal 10-His tag

Accession #

Q13489

Predicted Molecular Mass

71 kDa

Product Datasheets

E3-285

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-285

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 8.0, 500 mM NaCl, 10% (v/v) Glycerol, 5 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: cIAP-2/HIAP-1

Cellular inhibitor of apoptosis protein 2 (cIAP-2, also known as BIRC3, API2, and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Structurally, cIAP-2 is comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). The ring finger domain of cIAP-2 functions as an E3 ubiquitin ligase to ubiquitinate specific targets such as RIPK1-4, CASP3, 7, and 8, TRAF1, and BCL10. cIAP-2 is an important regulator of innate immune signaling via regulation of Toll-like receptors, Nod-like receptors and RIG-I receptors, collectively known as pattern recognitions receptors (PRRs).

References