Recombinant Human His10-cIAP-2/HIAP-1 Protein, CF

R&D Systems | Catalog # E3-285

R&D Systems
Discontinued Product
E3-285 has been discontinued. View all cIAP-2/HIAP-1 products.

Key Product Details

Source

E. coli

Accession Number

Q13489

Applications

Bioactivity
View all cIAP-2/HIAP-1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human cIAP-2/HIAP-1 protein
Contains an N-terminal 10-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

71 kDa

Activity

Recombinant Human cIAP-2/HIAP-1 is a RING finger Ubiquitin ligase (E3) that functions downstream of a Ubiquitin­activating (E1) enzyme and a Ubiquitin-­conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial cIAP-2 concentration of 0.2­-1 μM.

Formulation, Preparation, and Storage

E3-285
Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 500 mM NaCl, 10% (v/v) Glycerol, 5 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: cIAP-2/HIAP-1

Cellular inhibitor of apoptosis protein 2 (cIAP-2, also known as BIRC3, API2, and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases.  Structurally, cIAP-2 is comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD).  The ring finger domain of cIAP-2 functions as an E3 ubiquitin ligase to ubiquitinate specific targets such as RIPK1-4, CASP3, 7, and 8, TRAF1, and BCL10.  cIAP-2 is an important regulator of innate immune signaling via regulation of Toll-like receptors, Nod-like receptors and RIG-I receptors, collectively known as pattern recognitions receptors (PRRs).

References

  1. Bertrand M.J. et al.  (2011) PLoS ONE  6: E22356
  2. Mei Y. et al.  (2011)  J. Biol. Chem.  286: 35380
  3. Zhou A.Y. et al.  (2013) Cell Rep.  3: 724

Alternate Names

BIRC3, cIAP2, HIAP-1, MIHC

Entrez Gene IDs

330 (Human)

Gene Symbol

BIRC3

UniProt

Q13489

Additional cIAP-2/HIAP-1 Products

Product Documents for Recombinant Human His10-cIAP-2/HIAP-1 Protein, CF

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Product Specific Notices for Recombinant Human His10-cIAP-2/HIAP-1 Protein, CF

For research use only

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