Recombinant Human His10-CUL3/RBX1 Complex Protein, CF

Catalog # Availability Size / Price Qty
E3-435-025

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Recombinant Human His10-CUL3/RBX1 Complex Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL3/RBX1 Complex protein
Met1 - Ala768 with a N-terminal 10-His tag (CUL3); Met1 - His108 (RBX1)
Predicted Molecular Mass
92 kDa (CUL3), 12 kDa (RBX1)

Product Datasheets

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E3-435

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-435

Formulation Supplied as a solution in HEPES, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: CUL3/RBX1 Complex

Cullin-3 (CUL3) is a core component of multiple BCR (BTB-CUL3-RBX) E3 Ubiquitin ligase complexes that mediate the ubiquitination of several classes of signaling and structural proteins.  In the BCR complex, CUL3 serves as a scaffold that organizes one or more BTB (BR-C, Ttk and Bab, also known as a POZ domain) substrate recognition subunits with the RBX subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  Substrate specificity of a BCR ligase is determined by the BTB domain protein(s) associated with the ligase, though BTB-independent CUL3 ligase activity has been reported.  In vivo, the E3 ubiquitin ligase of the BCR complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  

References
  1. Baek, K., et al. (2020) Nature 578:461.
  2. Choo Y.Y and T. Hagen (2012) PLos One 7:e41350.
  3. Davidge, B., et al. (2019) J. Cell Sci. 132:jcs233049. 
  4. Duda, D.M., et al. (2012) Mol. Cell  47:371.
  5. Stogios, P.J. et al. (2005) Genome Biol. 6:R82.
Long Name
Cullin 3/Ring Box Protein 1
Alternate Names
CUL3/RBX1 Complex

Citation for Recombinant Human His10-CUL3/RBX1 Complex Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. G3BP1 inhibits Cul3SPOP to amplify AR signaling and promote prostate cancer
    Authors: C Mukhopadhy, C Yang, L Xu, D Liu, Y Wang, D Huang, LD Deonarine, J Cyrta, E Davicioni, A Sboner, BD Robinson, AM Chinnaiyan, MA Rubin, CE Barbieri, P Zhou
    Nature Communications, 2021-11-18;12(1):6662.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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