Ubiquitin-conjugating Enzyme H10 (UbcH10), also known as Ubiquitin-conjugating Enzyme E2C (UBE2C), is a 179 amino acid (aa) member of the yeast Ubc4/5 family of Ubiquitin-conjugating (E2) enzymes and has a predicted molecular weight of 20 kDa. Human UbcH10/UBE2C shares 96% aa sequence identity with mouse and rat UBE2C. UbcH10/UBE2C is an essential mediator of mitotic destruction events and cell cycle progression (1,2). UbcH10/UBE2C recognizes TEK sequences in target proteins such as Cyclins A and B, mediates Lys11-linked ubiquitination, and promotes target protein degradation in conjunction with APC/C, a Ubiquitin ligase (E3) (3). The catalytic activity of UbcH10/UBE2C is regulated by a conserved N-terminal extension, which mediates E2-E3 interaction (4). UbcH10/UBE2C is overexpressed in a variety of human cancers, and alternate splice isoforms may contribute to uncontrolled cell proliferation and tumor progression (5-8).
Recombinant Human His6-UbcH10/UBE2C Protein, CF
R&D Systems | Catalog # E2-650
Discontinued Product
E2-650 has been discontinued.
View all UbcH10/UBE2C products.
Key Product Details
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human UbcH10/UBE2C protein
Contains an N-terminal Met and 7-His tag
Contains an N-terminal Met and 7-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
21 kDa
Activity
Recombinant Human His6-UbcH10/UBE2C is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UbcH10/UBE2C concentration of 0.1-1 μM.
Formulation, Preparation, and Storage
E2-650
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: UbcH10/UBE2C
References
- Ye, Y. & M. Rape (2009) Nat. Rev. Mol. Cell Biol. 10:755.
- Lin, Y. et al. (2002) J. Biol. Chem. 277:21913.
- Dimova, N.V. et al. (2012) Nat. Cell Biol. 14:168.
- Summers, M.K. et al. (2008) Mol. Cell 31:544.
- Okamoto, Y. et al. (2003) Cancer Res. 63:4167.
- van Ree, J.H. et al. (2010) J. Cell Biol. 188:83.
- Jiang, L. et al. (2008) Brain Res. 1201:161.
- Hao, Z. et al. (2012) Tumor Biol. 33:723.
Alternate Names
UBE2C
Gene Symbol
UBE2C
UniProt
Additional UbcH10/UBE2C Products
Product Documents for Recombinant Human His6-UbcH10/UBE2C Protein, CF
Certificate of Analysis
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Product Specific Notices for Recombinant Human His6-UbcH10/UBE2C Protein, CF
For research use only
Citations for Recombinant Human His6-UbcH10/UBE2C Protein, CF
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Associated Pathways
