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Recombinant Human His6-UBE2D4 Protein, CF

Catalog #: E2-705
1 Citation Datasheet / COA / SDS

Discontinued Product

E2-705 has been discontinued.
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Recombinant Human His6-UBE2D4 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human His6-UBE2D4 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2D4 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2D4/Ubch5d protein
Contains an N-terminal 6-His tag
Accession #
Q9Y2X8
Predicted Molecular Mass
17 kDa

Product Datasheets

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E2-705

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-705

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2D4/Ubch5d

Ubiquitin-conjugating enzyme E2D 4 (UBE2D4), also known as Ubiquitin-conjugating Enzyme H5d (Ubch5d), is a member of the Ubiquitin-conjugating (E2) enzyme family. Human UBE2D4/Ubch5d is 147 amino acids in length and has a predicted molecular weight of approximately 16.5 kDa. It may function with the IDOL Ubiquitin ligase (E3) to ubiquitinate LDL R (1). UBE2D4/Ubch5d, along with other UBE2D family members, may also be involved in the degradation of p53 (2). Cadmium exposure has been reported to reduce the mRNA expression level of UBE2D4/Ubch5d (2). This protein contains an N-terminal 6-His tag.

References
  1. Zhang, L. et al. (2011) Genes Dev. 25:1262.
  2. Tokumoto, M. et al. (2011) J. Toxicol. Sci. 36:191.
Long Name
Ubiquitin-conjugating Enzyme E2D 4
Entrez Gene IDs
51619 (Human); 100041484 (Mouse); 79435 (Rat)
Alternate Names
EC 6.3.2.19; FLJ32004; HBUCE1; HBUCE1Ubiquitin carrier protein D4; Ubch5d; UBE2D4; ubiquitin-conjugating enzyme E2 D4; ubiquitin-conjugating enzyme E2D 4 (putative); ubiquitin-conjugating enzyme HBUCE1; Ubiquitin-protein ligase D4

Citation for Recombinant Human His6-UBE2D4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing SWI/SNF
    Authors: Radko-Juettner, S;Yue, H;Myers, JA;Carter, RD;Robertson, AN;Mittal, P;Zhu, Z;Hansen, BS;Donovan, KA;Hunkeler, M;Rosikiewicz, W;Wu, Z;McReynolds, MG;Roy Burman, SS;Schmoker, AM;Mageed, N;Brown, SA;Mobley, RJ;Partridge, JF;Stewart, EA;Pruett-Miller, SM;Nabet, B;Peng, J;Gray, NS;Fischer, ES;Roberts, CWM;
    Nature
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay

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