Ubiquitin-conjugating enzyme E2D 4 (UBE2D4), also known as Ubiquitin-conjugating Enzyme H5d (Ubch5d), is a member of the Ubiquitin-conjugating (E2) enzyme family. Human UBE2D4/Ubch5d is 147 amino acids in length and has a predicted molecular weight of approximately 16.5 kDa. It may function with the IDOL Ubiquitin ligase (E3) to ubiquitinate LDL R (1). UBE2D4/Ubch5d, along with other UBE2D family members, may also be involved in the degradation of p53 (2). Cadmium exposure has been reported to reduce the mRNA expression level of UBE2D4/Ubch5d (2). This protein contains an N-terminal 6-His tag.
Recombinant Human His6-UBE2D4 Protein, CF
R&D Systems | Catalog # E2-705
Discontinued Product
E2-705 has been discontinued.
View all UBE2D4/Ubch5d products.
Key Product Details
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human UBE2D4/Ubch5d protein
Contains an N-terminal 6-His tag
Contains an N-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
17 kDa
Activity
Recombinant Human His6-UBE2D4 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2D4 concentration of 0.1-1 μM.
Formulation, Preparation, and Storage
E2-705
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: UBE2D4/Ubch5d
References
- Zhang, L. et al. (2011) Genes Dev. 25:1262.
- Tokumoto, M. et al. (2011) J. Toxicol. Sci. 36:191.
Long Name
Ubiquitin-conjugating Enzyme E2D 4
Alternate Names
HBUCE1, Ubch5d
Gene Symbol
UBE2D4
UniProt
Additional UBE2D4/Ubch5d Products
Product Documents for Recombinant Human His6-UBE2D4 Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human His6-UBE2D4 Protein, CF
For research use only
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