Recombinant Human His6-UBE2E3 Protein, CF

Catalog # Availability Size / Price Qty
E2-678-100
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Recombinant Human His6-UBE2E3 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-Ube2E3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ube2E3 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2E3 protein
Contains an N-terminal 6-His tag
Accession #
Predicted Molecular Mass
25 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-678

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% Glycerol (v/v), 1mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: UBE2E3

Ubiquitin-conjugating Enzyme E2E 3 (UBE2E3), also known as Ubiquitin-conjugating Enzyme H9 (UbcH9), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). UBE2E3 has a predicted molecular weight of 23 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2E3 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (2). This enzyme appears to be localized in the nucleus of interphase cells and cell-wide in mitotic cells (3). UBE2E3 may be regulated at the post-transcriptional level by miR-143 (4). The auto-ubiquitination of the ARA54, RNF8, and bacterially-encoded NleG Ubiquitin ligases (E3s) is mediated by UBE2E3 (5,6). UBE2E3 also functions with NEDD4L to regulate ENaC activity (7). Physiologically, UBE2E3 is required for the proliferation of retinal pigment epithelial cells via the negative regulation of p27/Kip1 levels (3). UBE2E3 may also be required for HIV-1 replication (8). This protein has an N-terminal His6-tag. (Accession # NP_006348.1)

References
  1. Ito, K. et al. (1999) Cytogenet. Cell Genet. 84:99.
  2. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  3. Plafker, K.S. et al. (2008) Invest. Ophthalmol. Vis. Sci. 49:5611.
  4. Trakooljul, N. et al. (2010) Anim. Genet. 41:357.
  5. Ito, K. et al. (2001) Eur. J. Biochem. 268:2725.
  6. Wu, B. et al. (2010) PLoS Pathog. 6:e1000960.
  7. Debonneville, C. et al. (2004) Mol. Cell. Biol. 24:2397.
  8. Dziuba, N. et al. (2012) AIDS Res. Hum. Retroviruses 28:1329.
Entrez Gene IDs
10477 (Human); 22193 (Mouse); 295686 (Rat)
Alternate Names
E2-23K; EC 6.3.2.19; UBCE4; UbcH9; UBCH9ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5); UbcM2; UBE2E3; Ubiquitin carrier protein E3; ubiquitin-conjugating enzyme E2 E3; Ubiquitin-conjugating enzyme E2-23 kDa; ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase E3

Citation for Recombinant Human His6-UBE2E3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Virally Encoded DeSUMOylase Activity Is Required for Cytomegalovirus Reactivation from Latency
    Authors: EL Poole, VG Kew, JCH Lau, MJ Murray, T Stamminger, JH Sinclair, MB Reeves
    Cell Rep, 2018;24(3):594-606.
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human His6-UBE2E3 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human His6-UBE2E3 Protein, CF and earn rewards!

Have you used Recombinant Human His6-UBE2E3 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review