Recombinant Human His6-UBE2F Protein, CF

R&D Systems | Catalog # E2-679

Will be discontinued when existing inventory is gone.
R&D Systems

Key Product Details

Source

E. coli

Accession Number

NP_542409.1

Applications

Enzyme Activity
View all UBE2F Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human UBE2F protein
Met1 - Arg185 with a N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

23 kDa

Activity

Recombinant Human His6-UBE2F is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2F concentration of 0.1-1 μM.

Formulation, Preparation, and Storage

E2-679
Formulation Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: UBE2F

Ubiquitin-conjugating Enzyme E2F (UBE2F) is a NEDD8-conjugating enzyme (E2) (1). It is a 185 amino acid (aa) protein with a predicted molecular weight of 21 kDa. The human protein exhibits approximately 96% aa sequence identity with its mouse and rat orthologs. UBE2F is involved the covalent attachment of NEDD8 to target proteins in a process termed neddylation. For example, UBE2F appears to participate in the neddyylation of Cullin 5 (1). Members of the Cullin family (Cullin 1-7) are scaffold proteins that can incorporate into multimolecular complexes termed Cullin RING Ubiquitin Ligases (CRLs) (2). CRLs make up the largest subset of Ubiquitin Ligases (E3), and their activity is promoted by neddylation of associated Cullin family proteins (3).

References

  1. Huang, D.T. et al. (2009) Mol. Cell 33:483.
  2. Sarikas, A. et al. (2011) Genome Biol. 12:220.
  3. Duda, D.M. et al. (2011) Curr. Opin. Struct. Biol. 21:257.

Long Name

Ubiquitin-conjugating Enzyme E2F

Alternate Names

NCE2

Entrez Gene IDs

140739 (Human); 67921 (Mouse); 363284 (Rat)

Gene Symbol

UBE2F

UniProt

NP_542409.1 (Human)

Additional UBE2F Products

Product Documents for Recombinant Human His6-UBE2F Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-UBE2F Protein, CF

For research use only

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail