Recombinant Human His6-UBE2F Protein, CF

Please inquire regarding future lot manufacture; Will be discontinued when existing inventory is gone.

Catalog # Availability Size / Price Qty
E2-679-100
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human His6-UBE2F Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human His6-UBE2F is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2F concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2F protein
Met1 - Arg185 with a N-terminal 6-His tag
Accession #
Predicted Molecular Mass
23 kDa

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E2-679

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-679

Formulation Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: UBE2F

Ubiquitin-conjugating Enzyme E2F (UBE2F) is a NEDD8-conjugating enzyme (E2) (1). It is a 185 amino acid (aa) protein with a predicted molecular weight of 21 kDa. The human protein exhibits approximately 96% aa sequence identity with its mouse and rat orthologs. UBE2F is involved the covalent attachment of NEDD8 to target proteins in a process termed neddylation. For example, UBE2F appears to participate in the neddyylation of Cullin 5 (1). Members of the Cullin family (Cullin 1-7) are scaffold proteins that can incorporate into multimolecular complexes termed Cullin RING Ubiquitin Ligases (CRLs) (2). CRLs make up the largest subset of Ubiquitin Ligases (E3), and their activity is promoted by neddylation of associated Cullin family proteins (3).

References
  1. Huang, D.T. et al. (2009) Mol. Cell 33:483.
  2. Sarikas, A. et al. (2011) Genome Biol. 12:220.
  3. Duda, D.M. et al. (2011) Curr. Opin. Struct. Biol. 21:257.
Long Name
Ubiquitin-conjugating Enzyme E2F
Entrez Gene IDs
140739 (Human); 67921 (Mouse); 363284 (Rat)
Alternate Names
EC 6.3.2; EC 6.3.2.-; MGC18120; NCE2; NCE2Ubiquitin-conjugating enzyme E2 F; NEDD8 carrier protein UBE2F; NEDD8 conjugating enzyme; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; NEDD8-conjugating enzyme UBE2F; UBE2F; ubiquitin-conjugating enzyme E2F (putative)

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