Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF Summary
Recombinant Human His6-UBE2N/UBE2V2 Complex is a member of the Ubiquitin conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-UBE2N/UBE2V2 Complex concentration of 0.1-1 μM.
Contains an N-terminal 6-His tag
Accession # P61088 (UBE2N)/Q15819 (UBE2V2)
18 kDa (UBE2N), 17 kDa (UBE2V2)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||0.88 mg/ml (25 μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 2 mM TCEP|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: UBE2N/UBE2V2 Complex
Ubiquitin conjugating Enzyme E2N (UBE2N), also known as Ubiquitin conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive UBE2V2 (human homologue of yeast MMS2) protein (1). Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively, while human UBE2V2 shares 99% aa sequence identity with its mouse and rat orthologs. The UBE2N/UBE2V2 Complex functions with Ubiquitin ligases (E3s), including RNF111 and RNF8, to synthesize Lys63-linked Ubiquitin chains (2,3) that can either be unanchored or attached to target proteins (4, 5). The UBE2N/UBE2V2 complex has important roles in facilitating responses to various forms of DNA damage (2, 6).
Hofmann R.M.& Pickart C.M. (1999) Cell 96: 645
Poulsen, S.L et al. (2013) Journ Cell Biol. 201: 797
Campbell, S.J. et al. (2012) Journ. Biol. Chem. 287: 23900
Xia, Z.P. et al. (2009) Nature 461:114.
Liu, C. et al. (2009) Sci. Signal. 2:ra63.
Wen, R. et al. (2012) DNA Repair 11: 157
Citation for Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
PI5P4K? functions in DTX1-mediated Notch signaling
Authors: L Zheng, SD Conner
Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
Sample Types: Recombinant Protein
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