Recombinant Human His6-UBE2Q2 Isoform 1 Protein, CF

Catalog # Availability Size / Price Qty
E2-745-100
Product Details
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Recombinant Human His6-UBE2Q2 Isoform 1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-UBE2Q2-1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2Q2-1 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2Q2 protein
Contains an N-terminal 6-His tag
Accession #
Predicted Molecular Mass
44 kDa
SDS-PAGE
44 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-745

Formulation X mg/ml (X μM) in 50 mM HEPES, pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 2 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: UBE2Q2

Ubiquitin-conjugating Enzyme E2Q Family Member 2 (UBE2Q2) is a 375 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of approximately 43 kDa. Human UBE2Q2 shares 95% aa sequence identity with its mouse ortholog. UBE2Q2 contains an RWD domain, a coiled-coil domain, and an E2 catalytic core domain (1). The E2 cataylic core domain contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (2,3). UBE2Q2 expression has been observed in both the cytoplasm and the nucleus (1,4). Expression of dominant-negative UBE2Q2 can cause a prolonged prophase arrest and UBE2Q2 overexpression inhibits cell proliferation, suggesting that UBE2Q2 may be involved in normal cell cycle progression (3,4). Elevated expression of UBE2Q2 has been reported in hypopharyngeal cancer, breast cancer, and acute lymphoblastic leukemia, suggesting that this enzyme may function as an oncogene (1,4-6). This protein contains an N-terminal 6-His tag.

References
  1. Seghatoleslam, A. et al. (2006) Biochem. Biophys. Res. Commun. 339:422.
  2. Melner, M.H. et al. (2006) Biol. Reprod. 75:395.
  3. Banerjee, S. et al. (2007) Oncogene 26:6509.
  4. Maeda, H. et al. (2009) Mol. Cancer Res. 7:1553.
  5. Nikseresht, M. et al. (2010) Cancer Genet. Cytogenet. 197:101.
  6. Seghatoleslam, A. et al. (2012) Arch. Iran. Med. 15:352.
Long Name
Ubiquitin-conjugating Enzyme E2Q 2
Entrez Gene IDs
92912 (Human); 109161 (Mouse)
Alternate Names
DKFZp762C143; EC 6.3.2.19; LOC92912; UBE2Q2; Ubiquitin carrier protein Q2; ubiquitin-conjugating enzyme E2 Q2; ubiquitin-conjugating enzyme E2Q (putative) 2; ubiquitin-conjugating enzyme E2Q family member 2; Ubiquitin-protein ligase Q2

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