Recombinant Human His6-UBE2R2 Protein, CF Summary
Contains an N-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Ubiquitin-conjugating Enzyme E2R 2 (UBE2R2), also known as CDC34B and UBC3B, is a 238 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of 27 kDa. Human UBE2R2 shares 100% aa sequence identity with its mouse ortholog and 78% aa sequence identity with its paralog, UBCH3. UBE2Q2 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). The UBE1 Ubiquitin-activating (E1) enzyme, but not the UBA6 E1 enzyme, is capable of charging UBE2R2 with Ubiquitin (2). UBE2R2 can be phosphorylated on Ser233 by Casein Kinase 2, which promotes an interaction with the beta -TrCP Ubiquitin ligase (E3) and the subsequent ubiquitination and degradation of beta -Catenin (1). This protein contains an N-terminal 6-His tag.
Citation for Recombinant Human His6-UBE2R2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation
Authors: F Lampert, D Stafa, A Goga, MV Soste, S Gilberto, N Olieric, P Picotti, M Stoffel, M Peter
Sample Types: Recombinant Protein
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