Recombinant Human His6-UBE2R2 Protein, CF

Catalog # Availability Size / Price Qty
E2-715-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (1)
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Recombinant Human His6-UBE2R2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-UBE2R2 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2R2 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2R2 protein
Contains an N-terminal 6-His tag
Accession #
Predicted Molecular Mass
28 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-715

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: UBE2R2

Ubiquitin-conjugating Enzyme E2R 2 (UBE2R2), also known as CDC34B and UBC3B, is a 238 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of 27 kDa. Human UBE2R2 shares 100% aa sequence identity with its mouse ortholog and 78% aa sequence identity with its paralog, UBCH3. UBE2Q2 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). The UBE1 Ubiquitin-activating (E1) enzyme, but not the UBA6 E1 enzyme, is capable of charging UBE2R2 with Ubiquitin (2). UBE2R2 can be phosphorylated on Ser233 by Casein Kinase 2, which promotes an interaction with the beta -TrCP Ubiquitin ligase (E3) and the subsequent ubiquitination and degradation of beta -Catenin (1). This protein contains an N-terminal 6-His tag.

References
  1. Semplici, F. et al. (2002) Oncogene 21:3978.
  2. Jin, J. et al. (2007) Nature 447:1135.
Long Name
Ubiquitin-conjugating Enzyme E2R 2
Entrez Gene IDs
54926 (Human); 67615 (Mouse); 689226 (Rat)
Alternate Names
CDC34B; CDC34BEC 6.3.2.19; E2-CDC34B; FLJ20419; MGC10481; UBC3B; UBC3BUbiquitin-protein ligase R2; UBE2R2; Ubiquitin carrier protein R2; ubiquitin-conjugating enzyme E2 R2; Ubiquitin-conjugating enzyme E2-CDC34B; ubiquitin-conjugating enzyme E2R 2; ubiquitin-conjugating enzyme UBC3B

Citation for Recombinant Human His6-UBE2R2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation
    Authors: F Lampert, D Stafa, A Goga, MV Soste, S Gilberto, N Olieric, P Picotti, M Stoffel, M Peter
    Elife, 2018;7(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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