Recombinant Human His6-UBE2W Isoform 1 Protein, CF

Catalog # Availability Size / Price Qty
E2-740-100
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Recombinant Human His6-UBE2W Isoform 1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Activity
Recombinant Human His6-UBE2W-1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2W-1 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2W protein
Contains an N-terminal 6-His tag
Accession #
Predicted Molecular Mass
18 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-740

Formulation X mg/ml (X μM) in 50 mM MOPS pH 6.5, 150 mM NaCl and 2 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

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Background: UBE2W

Ubiquitin-conjugating Enzyme E2W (UBE2W), also known as Ubiquitin-conjugating Enzyme 16 (UBC16), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). It has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1,2). There are two isoforms of UBE2W, isoform 1 and isoform 2, with predicted molecular weights of 18.1 kDa and 19.5 kDa, respectively. UBE2W localizes to the nucleus where it promotes the mono-ubiquitination of BRCA1 and FANCD2, both of which contribute to DNA repair pathways (1-4). Mono-ubiquitination of the CHIP Ubiquitin ligase (E3), which has been reported to enhance its E3 ligase activity, is also mediated by UBE2W (5). This protein contains an N-terminal 6-His tag.

References
  1. Yin, G. et al. (2006) Front. Biosci. 11:1500.
  2. Alpi, A.F. et al. (2008) Mol. Cell 32:767.
  3. Christensen, D.E. et al. (2007) Nat. Struct. Mol. Biol. 14:941.
  4. Zhang, Y. et al. (2011) Mol. Cells 31:113.
  5. Scaglione, K.M. et al. (2011) Mol. Cell 43:599.
Long Name
Ubiquitin-conjugating Enzyme E2W
Entrez Gene IDs
55284 (Human); 66799 (Mouse)
Alternate Names
EC 6.3.2.19; FLJ11011; hUBC-16; probable ubiquitin-conjugating enzyme E2 W; Ubc16; UBE2W; Ubiquitin carrier protein W; ubiquitin-conjugating enzyme 16; ubiquitin-conjugating enzyme E2W (putative); Ubiquitin-protein ligase W

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