Ubiquitin-conjugating Enzyme E2W (UBE2W), also known as Ubiquitin-conjugating Enzyme 16 (UBC16), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). It has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1,2). There are two isoforms of UBE2W, isoform 1 and isoform 2, with predicted molecular weights of 18.1 kDa and 19.5 kDa, respectively. UBE2W localizes to the nucleus where it promotes the mono-ubiquitination of BRCA1 and FANCD2, both of which contribute to DNA repair pathways (1-4). Mono-ubiquitination of the CHIP Ubiquitin ligase (E3), which has been reported to enhance its E3 ligase activity, is also mediated by UBE2W (5). This protein contains an N-terminal 6-His tag.
Recombinant Human His6-UBE2W Isoform 2 Protein, CF
R&D Systems | Catalog # E2-725
Discontinued Product
E2-725 has been discontinued.
View all UBE2W products.
Key Product Details
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human UBE2W protein
Contains an N-terminal 6-His tag
Contains an N-terminal 6-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
19 kDa
Activity
Recombinant Human His6-UBE2W-2 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2W-2 concentration of 0.1-1 μM.
Formulation, Preparation, and Storage
E2-725
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: UBE2W
References
- Yin, G. et al. (2006) Front. Biosci. 11:1500.
- Alpi, A.F. et al. (2008) Mol. Cell 32:767.
- Christensen, D.E. et al. (2007) Nat. Struct. Mol. Biol. 14:941.
- Zhang, Y. et al. (2011) Mol. Cells 31:113.
- Scaglione, K.M. et al. (2011) Mol. Cell 43:599.
Long Name
Ubiquitin-conjugating Enzyme E2W
Alternate Names
Ubc16
Gene Symbol
UBE2W
UniProt
Additional UBE2W Products
Product Documents for Recombinant Human His6-UBE2W Isoform 2 Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human His6-UBE2W Isoform 2 Protein, CF
For research use only
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Associated Pathways
