Ubiquitin-conjugating Enzyme Variant 1a (Uev1a), also known as Ubiquitin-conjugating Enzyme E2 Variant 1 (UBE2V1), is a member of the Ubiquitin-conjugating (E2) enzyme family. Human Uev1a/UBE2V1 and its mouse ortholog have predicted molecular weights of 19 kDa and 16.5 kDa, respectively. The human protein shares 89% amino acid (aa) sequence identity with its mouse ortholog. The E2 catalytic core domain of Uev1a/UBE2V1 lacks an active site cysteine residue, rendering it catalytically inactive on its own (1). However, in the cytoplasm Uev1a/UBE2V1 is able to form a catalytically active complex with UBE2N/Ubc13, which mediates the synthesis Lys63-linked Ubiquitin chains and is required for NF-kappa B activation (2,3). Uev1a/UBE2V1 is required for UBE2N (Ubc13)/Uev1a Complex-dependent Lys63-linked Ubiquitin chain formation. More specifically, Uev1a/UBE2V1 orients the Ubiquitin molecule to favor linkage at Lys63 via a non-covalent interaction with the Ubiquitin molecule (4). The human protein contains a non-conserved 30 aa N-terminal tail that functionally differentiates it from Mms2, another E2 enzyme variant and UBE2N/Ubc13 binding partner (4). Pharmacological inhibition of the UBE2N (Ubc13)/Uev1a Complex blocks the proliferation of diffuse large B-cell lymphoma cells, suggesting that this complex may play a role in cancer (5). This protein has an N-terminal His6-tag.
Recombinant Human His6-Uev1a (Mms2)/UBE2V1 Protein, CF
R&D Systems | Catalog # E2-662
Discontinued Product
E2-662 has been discontinued.
View all Uev1a/UBE2V1 products.
Key Product Details
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human Uev1a/UBE2V1 protein
Contains a 6-His tag
Contains a 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human His6-Uev1a (Mms2)/UBE2V1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Uev1a (Mms2)/UBE2V1 concentration of 0.1-1 μM.
Reviewed Applications
Read 1 review rated 5 using E2-662 in the following applications:
Formulation, Preparation, and Storage
E2-662
| Formulation | Supplied as a solution in 50 mM Hepes pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 2 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Uev1a/UBE2V1
References
- Broomfield, S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5678.
- Andersen, P.L. et al. (2005) J. Cell Biol. 170:745.
- Pertel, T. et al. (2011) Nature 472:361.
- McKenna, S. et al. (2003) J. Biol. Chem. 278:13151.
- Pulvino, M. et al. (2012) Blood 120:1668.
Alternate Names
CIR1, CROC1, UBE2V1
Gene Symbol
UBE2V1
UniProt
Additional Uev1a/UBE2V1 Products
Product Documents for Recombinant Human His6-Uev1a (Mms2)/UBE2V1 Protein, CF
Certificate of Analysis
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Product Specific Notices for Recombinant Human His6-Uev1a (Mms2)/UBE2V1 Protein, CF
For research use only
Related Research Areas
Customer Reviews for Recombinant Human His6-Uev1a (Mms2)/UBE2V1 Protein, CF (1)
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