Recombinant Human His6-Ufc1 Protein, CF

R&D Systems | Catalog # E2-675

R&D Systems
Discontinued Product
E2-675 has been discontinued. View all Ufc1 products.

Key Product Details

Source

E. coli

Accession Number

Q9Y3C8

Applications

Enzyme Activity
View all Ufc1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Ufc1 protein
Contains an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

22 kDa

Activity

Recombinant Human His6-Ufc1 is a member of the UFM1-conjugating (E2) enzyme family that receives UFM1 from a UFM1-activating (E1) enzyme and subsequently interacts with a UFM1 ligase (E3) to conjugate UFM1 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ufc1 concentration of 0.1-1 μM.

Formulation, Preparation, and Storage

E2-675
Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Ufc1

Ubiquitin-fold Modifier Conjugating Enzyme 1 (Ufc1) has been identified as an E2-like conjugating enzyme for Ubiquitin Fold Modifier 1 (Ufm1) (1). Ufc1 is a 116 amino acid (aa) protein with a predicted molecular weight of 19 kDa. Human Ufc1 exhibits 97% and 98% aa identity to its mouse and rat orthologs, respectively. The process of attaching Ufm1 to target proteins is termed ufmylation. It is thought to occur in a manner analogous to the ubiquitin system, including stepwise enzymatic reactions involving Ubiquitin-activating (E1)-, Ubiquiting-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. In addition to Ufc1, other members of the Ufm1-conjugation system include the E1-like enzyme Uba5, E3-like enzyme Ufl1, and deconjugating enzymes UfSP1 and UfSP2 (1-3). This protein contains an N-terminal His6 tag.

References

  1. Komatsu, J. et al. (2004) EMBO J. 23:1977.
  2. Tatsumi, K. et al. (2010) 285:5417.
  3. Kang, S.H. et al. (2007) J. Biol. Chem. 282:5256.

Long Name

Ubiquitin-fold Modifier Conjugating Enzyme 1

Alternate Names

HSPC155

Entrez Gene IDs

51506 (Human); 66155 (Mouse); 445268 (Rat)

Gene Symbol

UFC1

UniProt

Q9Y3C8 (Human)

Additional Ufc1 Products

Product Documents for Recombinant Human His6-Ufc1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-Ufc1 Protein, CF

For research use only

Citations for Recombinant Human His6-Ufc1 Protein, CF

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