Recombinant Human His6-Ufc1 Protein, CF

Catalog # Availability Size / Price Qty
E2-675-100
Product Details
Citations (1)
FAQs
Reviews

Recombinant Human His6-Ufc1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-Ufc1 is a member of the UFM1-conjugating (E2) enzyme family that receives UFM1 from a UFM1-activating (E1) enzyme and subsequently interacts with a UFM1 ligase (E3) to conjugate UFM1 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ufc1 concentration of 0.1-1 μM.
Source
E. coli-derived human Ufc1 protein
Contains an N-terminal 6-His tag
Accession #
Predicted Molecular Mass
22 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-675

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: Ufc1

Ubiquitin-fold Modifier Conjugating Enzyme 1 (Ufc1) has been identified as an E2-like conjugating enzyme for Ubiquitin Fold Modifier 1 (Ufm1) (1). Ufc1 is a 116 amino acid (aa) protein with a predicted molecular weight of 19 kDa. Human Ufc1 exhibits 97% and 98% aa identity to its mouse and rat orthologs, respectively. The process of attaching Ufm1 to target proteins is termed ufmylation. It is thought to occur in a manner analogous to the ubiquitin system, including stepwise enzymatic reactions involving Ubiquitin-activating (E1)-, Ubiquiting-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. In addition to Ufc1, other members of the Ufm1-conjugation system include the E1-like enzyme Uba5, E3-like enzyme Ufl1, and deconjugating enzymes UfSP1 and UfSP2 (1-3). This protein contains an N-terminal His6 tag.

References
  1. Komatsu, J. et al. (2004) EMBO J. 23:1977.
  2. Tatsumi, K. et al. (2010) 285:5417.
  3. Kang, S.H. et al. (2007) J. Biol. Chem. 282:5256.
Long Name
Ubiquitin-fold Modifier Conjugating Enzyme 1
Entrez Gene IDs
51506 (Human); 66155 (Mouse); 445268 (Rat)
Alternate Names
HSPC155; ubiquitin-fold modifier conjugating enzyme 1; ubiquitin-fold modifier-conjugating enzyme 1; Ufc1; Ufm1-conjugating enzyme 1

Citation for Recombinant Human His6-Ufc1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination
    Authors: J Liu, D Guan, M Dong, J Yang, H Wei, Q Liang, L Song, L Xu, J Bai, C Liu, J Mao, Q Zhang, J Zhou, X Wu, M Wang, YS Cong
    Nat. Cell Biol., 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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