Recombinant Human His6-Use1/UBE2Z Protein, CF

R&D Systems | Catalog # E2-677

R&D Systems
Discontinued Product
E2-677 has been discontinued. View all Use1/UBE2Z products.

Key Product Details

Source

E. coli

Accession Number

Q9H832

Applications

Enzyme Activity
View all Use1/UBE2Z Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Use1/UBE2Z protein
Contains an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

41 kDa

Activity

Recombinant Human His6-Use1/UBE2Z is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Use1/UBE2Z concentration of 0.1-1 μM.

Formulation, Preparation, and Storage

E2-677
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Use1/UBE2Z

Uba6-specific E2 Conjugating Enzyme 1 (Use1), also known as Ubiquitin-conjugating Enzyme E2Z (UBE2Z), is a member of the Ubiquitin-conjugating (E2) enzyme family that is highly expressed in human placenta, pancreas, spleen, and testis (1). Use1/UBE2Z has a predicted molecular weight of 38 kDa. The human enzyme shares 98% amino acid sequence identity with its mouse and rat orthologs. It has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (2). Use1/UBE2Z functions specifically with the UBE1L2/UBA6 Ubiquitin-activating (E1) enzyme, rather than the UBE1 E1 enzyme, for Ubiquitin conjugation (3). Use1/UBE2Z expression is enriched in the cytoplasm where it mediates the ubiquitination of RGS4 and RGS5 via the N-end rule proteolytic pathway (4). Use1/UBE2Z also functions with UBE1L2/UBA6 to mediate the conjugation of HLA F-associated Transcript 10 (FAT10), a Ubiquitin-like protein, and is auto-FAT10ylated in response to TNF-alpha and IFN-gamma (5). This protein has an N-terminal His6-tag.

References

  1. Gu, X. et al. (2007) Mol. Biol. Rep. 34:183.
  2. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  3. Jin, J. et al. (2007) Nature 447:1135.
  4. Lee, P.C. et al. (2011) Mol. Cell 43:392.
  5. Aichem, A. et al. (2010) Nat. Commun. 4:13.

Alternate Names

UBE2Z

Entrez Gene IDs

65264 (Human)

Gene Symbol

UBE2Z

UniProt

Q9H832 (Human)

Additional Use1/UBE2Z Products

Product Documents for Recombinant Human His6-Use1/UBE2Z Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-Use1/UBE2Z Protein, CF

For research use only

Citations for Recombinant Human His6-Use1/UBE2Z Protein, CF

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