Recombinant Human His6-USP10 Protein, CF
Recombinant Human His6-USP10 Protein, CF Summary
Product Specifications
Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-USP10 concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555).
Contains an C-terminal 6-His tag
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Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-592
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: USP10
Ubiquitin carboxyl-terminal hydrolase 10 (USP10) is a specialized cysteine protease with a predicted molecular weight of 87 kDa. USP10 is a member of the peptidase C19 family and the human protein shares 83% amino acid sequence identity with its mouse ortholog. Reported USP10 targets include p53, BECN1, SNX3 and CFTR. USP10 translocates to the nucleus in response to DNA damage and deubiquitinates p53, thus regulating the p53-dependent DNA damage response. USP10 deubiquitination of BECN1 leads to stabilization of PIK3C3/VPS34-containing complexes which drives autophagy. The small molecule Spautin-1 exerts its potent inhibitory effects on autophagy partially through the inhibition of USP10 activity.
- Boulkroun S., et al. (2008) Am. J. Physiol. 295:F889-F900
- Liu J., et al. (2011) Cell 147: 223
- Yuan J., et al. (2010) Cell 140: 384
Citations for Recombinant Human His6-USP10 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The ubiquitin isopeptidase USP10 deubiquitinates LC3B to increase LC3B levels and autophagic activity
Authors: R Jia, JS Bonifacino
The Journal of Biological Chemistry, 2021;0(0):100405.
Species: Human
Sample Types: Cell Lysates
Applications: Deubiquitination -
Inhibition of the deubiquitinase USP10 induces degradation of SYK
Authors: J Yang, C Meng, E Weisberg, A Case, I Lamberto, RS Magin, S Adamia, J Wang, N Gray, S Liu, R Stone, M Sattler, S Buhrlage, JD Griffin
Br. J. Cancer, 2020;0(0):.
Species: Human
Sample Types: Protein
Applications: Bioassay
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