Recombinant Human His6-USP10 Protein, CF

R&D Systems | Catalog # E-592

R&D Systems
Discontinued Product
E-592 has been discontinued. View all USP10 products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

Q14694.2

Applications

Bioactivity
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Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP10 protein
Met1 - Leu798 with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

89 kDa

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-USP10 concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates ((Catalog # U-550 or U-555)).

Reviewed Applications

Read 1 review rated 5 using E-592 in the following applications:

Formulation, Preparation, and Storage

E-592
Formulation Supplied as a solution in HEPES, NaCl, TCEP, and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP10

Ubiquitin carboxyl-terminal hydrolase 10 (USP10) is a specialized cysteine protease with a predicted molecular weight of 87 kDa. USP10 is a member of the peptidase C19 family and the human protein shares 83% amino acid sequence identity with its mouse ortholog. Reported USP10 targets include p53, BECN1, SNX3 and CFTR. USP10 translocates to the nucleus in response to DNA damage and deubiquitinates p53, thus regulating the p53-dependent DNA damage response. USP10 deubiquitination of BECN1 leads to stabilization of PIK3C3/VPS34-containing complexes which drives autophagy. The small molecule Spautin-1 exerts its potent inhibitory effects on autophagy partially through the inhibition of USP10 activity.

References

  1. Boulkroun S., et al. (2008) Am. J. Physiol. 295:F889.
  2. Liu J., et al. (2011) Cell 147: 223.
  3. Yuan J., et al. (2010) Cell 140: 384.

Long Name

Ubiquitin Specific Protease 10

Alternate Names

Ubiquitin Thiolesterase 10, UBPO, Uchrp

Entrez Gene IDs

9100 (Human); 22224 (Mouse); 307905 (Rat)

Gene Symbol

USP10

UniProt

Q14694.2

Additional USP10 Products

Product Documents for Recombinant Human His6-USP10 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human His6-USP10 Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Recombinant Human His6-USP10 Protein, CF

Customer Reviews for Recombinant Human His6-USP10 Protein, CF (1)

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    Application: Enzymatic activity in vitro
    Verified Customer | Posted 01/21/2021

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