Ubiquitin carboxyl-terminal hydrolase 12 (USP12) is a specialized cysteine protease with a predicted molecular weight of 43 kDa. USP12 is a member of the peptidase C19 family and USP12/USP46 subfamily, and the human protein shares 98% amino acid sequence identity with its mouse ortholog. Like USP1 and USP46, USP12 lacks appreciable activity in the absence of its co-activator UAF1. Addition of UAF1 increases the in vitro activity of USP12 20-fold or more. WDR20 is often found in complex with both USP12 and UAF1, and the ternary complex is reported to be more active than the USP12/UAF1 complex. Biologically, USP12 has been implicated as a positive regulator of Androgen Receptor (AR) activity via a mechanism that involves down-regulation of Akt activity. As such, it has been proposed that USP12 inhibition could offer a therapeutic target for some forms of prostate cancer. This recombinant protein contains a C-terminal 6-His tag.
Recombinant Human His6-USP12 Protein, CF
R&D Systems | Catalog # E-578
Key Product Details
Source
Accession Number
Applications
Product Specifications
Source
Contains a C-terminal 6-His tag
Purity
Predicted Molecular Mass
Activity
Recombinant Human USP12 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP12 concentration of 50-500 nM. Requires a 1:1 stoichiometric amount of UAF1 (E-566) for activity when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) as a substrate. In vitro, the USP12/UAF1 complex will not efficiently hydrolyze poly-Ubiquitin chains in the absence of WDR20.
Formulation, Preparation, and Storage
E-578
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.2, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: USP12
References
- Burska, U.L. et al. (2013) J Biol Chem. 288: 32641
- Cohn, M.A. et al. (2009) J Biol Chem. 284: 5343
- Gangula, N.R. & Maddika, S. (2013) J Biol Chem. 288: 34545
- Kee, Y. et al. (2010) J Biol Chem. 285: 11252
- McClurg, U.L. et al. (2014) Oncotarget 5: 7081
- Ohashi, M. et al. (2015) PLoS Pathog. DOI: 10.1371/journal.ppat.1004822
Long Name
Alternate Names
Gene Symbol
UniProt
Additional USP12 Products
Product Documents for Recombinant Human His6-USP12 Protein, CF
Certificate of Analysis
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Product Specific Notices for Recombinant Human His6-USP12 Protein, CF
For research use only
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