Recombinant Human His6-USP15 Protein, CF

R&D Systems | Catalog # E-594

R&D Systems
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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human His6-USP15 Protein (E-594)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

Q9Y4E8.3

Applications

Bioactivity
View all USP15 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP15 protein
Met1 - Asn981 with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

113 kDa

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP15 concentration of 5-20 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555).

Formulation, Preparation, and Storage

E-594
Formulation Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP15

Ubiquitin carboxyl-terminal hydrolase 15 (USP15) is a specialized cysteine protease with a predicted molecular weight of 112 kDa.  USP15 is a member of the peptidase C19 family and the human protein shares 98% amino acid sequence identity with its mouse ortholog.  USP15 is a regulator of the TGF beta signaling pathway, where it may promote signaling strength by deubiquitination of SMAD proteins and/or the TGF beta R1.  Other reported targets of USP15 include histone H2B, NF kappa BI alpha, MDM2 and human papillomavirus type 16 E6 protein.

References

 
  1. Eichhorn P.J., et al. (2012) Nat. Med. 18: 429.
  2. Long L., et al. (2014) J. Biol. Chem. 289: 8916.
  3. Voss R.M., et al. (2009) J. Virol. 83: 8885.
  4. Zou Q., et al. (2014) Nat. Immunol. 15: 562.
 

Long Name

Ubiquitin Specific Protease 15

Alternate Names

Gcap18, KIAA0529, Ubp109, UNPH-2, UNPH4

Entrez Gene IDs

9958 (Human); 14479 (Mouse); 171329 (Rat)

Gene Symbol

USP15

UniProt

Q9Y4E8.3

Additional USP15 Products

Product Documents for Recombinant Human His6-USP15 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human His6-USP15 Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Recombinant Human His6-USP15 Protein, CF

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