Recombinant Human His6-USP2 Catalytic Domain Protein, CF

Catalog # Availability Size / Price Qty
E-506-050
Product Details
Citations (1)
FAQs
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Recombinant Human His6-USP2 Catalytic Domain Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human USP2- His6 Catalytic Domain is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP2- His6 Catalytic Domain concentration of 1-5 nM.
Source
E. coli-derived human USP2 protein
Contains a C-terminal 6-His tag
Accession #
Predicted Molecular Mass
41 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-506

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: USP2

Ubiquitin Specific Peptidase 2 (USP2), also known as UBP41 and USP9, is a cytoplasmic protein that belongs to the peptidase C19 family of deubiquitinating enzymes (1). It is widely expressed and serves to deubiquitinate select target substrates. At least three isoforms have thus far been described: USP2a, also known as USP2-69, USP-41, and USP2-45 (2,3). All isoforms contain the same catalytic core, but show variations within their N- and C-terminal domains (3). USP2a is the canonical isoform. It is 605 amino acids (aa) in length and has a predicted molecular weight of 68.1 kDa. Human USP2a shares 90% aa sequence identity with the mouse and rat orthologs. USP-41 and USP-45 are 362 and 396 aa in length, respectively, and have predicted molecular weights of 41.6 and 45.2 kDa. USP-41 and USP2a have been shown to be critical regulators of apoptosis and cell proliferation. They deubiquitinate poly-ubiquitinated target proteins, such as FAS, MDM2, and Cyclin D1, to promote or block apoptosis, depending on the context (3-9). USP-45, whose expression appears to be regulated by circadian and nutritional cues, is thought to regulate hepatic gluconeogenesis and glucose metabolism (10,11). This recombinant human protein encompasses the catalytic domain of USP2 (aa 259-605) and contains a C-terminal His6-tag. Accession # NP_004196.

References
  1. Baek, S.H. et al. (1997) J. Biol. Chem. 272:25560.
  2. Gousseva, N. & R.T. Baker (2003) Gene Expr. 11:163.
  3. Haimerl, F. et al. (2009) J. Biol. Chem. 284:495.
  4. Gewies, A. & S. Grimm (2003) Cancer Res. 63:682.
  5. Graner, E. et al. (2004) Cancer Cell 5:253.
  6. Stevenson, L.F. et al. (2007) EMBO J. 26:976.
  7. Shan, J. et al. (2009) Mol. Cell 36:469.
  8. Mahul-Mellier, A.L. et al. (2012) Cell Death Differ. 19:891.
  9. Mahul-Mellier, A.L. et al. (2012) Biochim. Biophys. Acta. 1823:1353.
  10. Molusky, M.M. et al. (2012) Diabetes 61:1025.
  11. Molusky, M.M. et al. (2012) PLoS One 7:e47970.
Long Name
Ubiquitin-specific Protease 2
Entrez Gene IDs
9099 (Human); 53376 (Mouse); 115771 (Rat)
Alternate Names
Deubiquitinating enzyme 2; EC 3.1.2.15; EC 3.4.19.12,41 kDa ubiquitin-specific protease; ubiquitin specific peptidase 2; ubiquitin specific protease 12; ubiquitin specific protease 2; ubiquitin specific protease 9; ubiquitin thioesterase 2; Ubiquitin thiolesterase 2; Ubiquitin-specific-processing protease 2; UBP41; UBP41ubiquitin carboxyl-terminal hydrolase 2; USP2; USP9

Citation for Recombinant Human His6-USP2 Catalytic Domain Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKC? Kinase Activity
    Authors: E Naik, VM Dixit
    J Immunol, 2016;196(8):3438-51.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay

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