Ubiquitin carboxyl-terminal hydrolase 4 (USP4) is a specialized cysteine protease with a predicted molecular weight of 109 kDa. USP4 is a member of the peptidase C19 family and the human protein shares 91% amino acid sequence identity with its mouse ortholog. USP4 is ubiquitinated on multiple sites, and deubiquitination of these sites requires USP4 catalytic activity. USP4 interacts with CtIP protein and the MRN (MRE11-RAD50-NBS1) complex to promote DNA double-strand break repair. Other reported USP4 targets include HDAC2, ADORA2A, PDPK1 and TRIM21.
Recombinant Human His6-USP4 Protein, CF
R&D Systems | Catalog # E-596
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Key Product Details
- R&D Systems Sf 21 (baculovirus)-derived Recombinant Human His6-USP4 Protein (E-596)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Sf 21 (baculovirus)
Accession Number
Applications
Bioactivity
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Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP4 protein
Met1 - Asn963 with a C-terminal 6-His tag
Met1 - Asn963 with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
109 kDa
Activity
Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP4 concentration of 10-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)
Formulation, Preparation, and Storage
E-596
| Formulation | Supplied as a solution in HEPES, NaCl, TCEP and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: USP4
References
- Li, Z., et al. (2016) Oncogene 35:2902.
- Milojevic, T., et al. (2006) Mol. Pharmacol. 69:1083.
- Wada, K. & T. Kamitani (2006) Biochem. Biophys. Res. Commun. 342:253.
- Wijnhoven, P., et al. (2015) Mol. Cell 60:362.
Long Name
Ubiquitin Specific Protease 4
Alternate Names
UNP, Unph
Gene Symbol
USP4
UniProt
Additional USP4 Products
Product Documents for Recombinant Human His6-USP4 Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human His6-USP4 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human His6-USP4 Protein, CF
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