Recombinant Human His6-USP4 Protein, CF

Catalog # Availability Size / Price Qty
E-596-050
Product Details
FAQs
Reviews

Recombinant Human His6-USP4 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Reaction conditions will need to be optimized for each specific application.  We recommend an initial recombinant human USP4 concentration of 10-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP4 protein
Contains an C-terminal 6-His tag
Accession #
Predicted Molecular Mass
109 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-596

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP, 10% (v/v) Glycerol
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: USP4

Ubiquitin carboxyl-terminal hydrolase 4 (USP4) is a specialized cysteine protease with a predicted molecular weight of 109 kDa. USP4 is a member of the peptidase C19 family and the human protein shares 91% amino acid sequence identity with its mouse ortholog. USP4 is ubiquitinated on multiple sites, and deubiquitination of these sites requires USP4 catalytic activity. USP4 interacts with CtIP protein and the MRN (MRE11-RAD50-NBS1) complex to promote DNA double-strand break repair. Other reported USP4 targets include HDAC2, ADORA2A, PDPK1 and TRIM21. This recombinant protein contains a C-terminal 6-His tag.

References
  1. Li Z., et al. (2015) Oncogene doi: 10.1038/onc.2015.349
  2. Milojevic T., et al. (2006) Mol. Pharmacol. 69: 1083
  3. Wada K. & Kamitani T. (2006) Biochem. Biophys. Res. Commun. 342:253
  4. Wijnhoven P., et al. (2015) Mol. Cell 60: 362
Long Name
Ubiquitin Specific Protease 4
Entrez Gene IDs
7375 (Human); 22258 (Mouse); 290864 (Rat)
Alternate Names
Deubiquitinating enzyme 4; EC 3.1.2.15; EC 3.4.19.12; MGC149848; MGC149849; ubiquitin carboxyl-terminal esterase 4; ubiquitin carboxyl-terminal hydrolase 4; ubiquitin specific peptidase 4 (proto-oncogene); ubiquitin specific protease 4 (proto-oncogene); ubiquitin thioesterase 4; Ubiquitin thiolesterase 4; ubiquitin-specific processing protease 4; Ubiquitin-specific-processing protease 4; Ubiquitous nuclear protein homolog; UNP; Unph; UNPUnph; USP4

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human His6-USP4 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human His6-USP4 Protein, CF and earn rewards!

Have you used Recombinant Human His6-USP4 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review