Recombinant Human His6-USP9x Isoform 2 Protein, CF

R&D Systems | Catalog # E-552

 
R&D Systems
Discontinued Product
E-552 has been discontinued. View all USP9x products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

NP_001034680

Applications

Enzyme Activity
View all USP9x Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP9x protein
Contains an N-terminal Met and 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

291 kDa

Activity

Recombinant Human His6-USP9x-2 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-USP9x-2 concentration of 0.05-0.5 μM.

Formulation, Preparation, and Storage

E-552
Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 5 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP9x

Ubiquitin Specific Peptidase 9, X-linked (USP9x), also known as DFFRX, FAF, and FAM, is a cytoplasmic protein that belongs to the peptidase C19 family of deubiquitinating enzymes (1,2). It is 2570 amino acids (aa) in length with a predicted molecular weight of 292.3 kDa. Human USP9x shares 98% aa sequence identity with the mouse and rat orthologs. USP9x contains a catalytic domain located between aa 1531 and 1971 and a Ubiquitin-like domain that is located at aa 873-963 (3). It is widely expressed and has been shown to interact with and deubiquitinate multiple substrates. Some putative targets include beta -Catenin, TOR, Synuclein-alpha, the Ubiquitin ligases (E3s) Itch, MARCH7, and SMURF1, and the MAP kinase kinase kinase ASK1 (4-10). USP9x has also been shown to control the activity of the AMPK-related kinases ARK5 and MARK4 through direct removal of Lys29- and Lys33-linked Ubiquitin chains (3). It has also been shown to promote cell survival by deubiquitinating and stabilizing the anti-apoptotic protein Mcl-1, and to regulate chromosome alignment and segregation during mitosis by deubiquitinating the chromosome passenger protein Survivin (11-13). Additionally, USP9x appears to promote TGF-beta signaling via deubiquitination of mono-ubiquitinated Smad4 (14). USP9x expression has been shown to be correlated with several cancer types (11,15,16). This recombinant human protein contains an N-terminal His6-tag.

References

  1. Jones, M.H. et al. (1996) Hum. Mol. Genet. 5:1695.
  2. Wood, S.A. et al. (1997) Mech. Dev. 63:29.
  3. Al-Hakim, A.K. et al. (2008) Biochem. J. 411:249.
  4. Murray, R.Z. et al. (2004) Mol. Biol. Cell 15:1591.
  5. Mouchantaf, R. et al. (2006) J. Biol. Chem. 281:38738.
  6. Nathan, J.A. et al. (2008) Traffic 9:1130.
  7. Nagai, H. et al. (2009) Mol. Cell 36:805.
  8. Rott, R. et al. (2011) Proc. Natl. Acad. Sci. USA 108:18666.
  9. Agrawal, P. et al. (2012) J. Biol. Chem. 287:21164.
  10. Xie, Y. et al. (2013) J. Biol. Chem. 288:2976.
  11. Schwickart, M. et al. (2010) Nature 463:103.
  12. Trivigno, D. et al. (2012) Neoplasia 14:893.
  13. Vong, Q.P. et al. (2005) Science 310:1499.
  14. Dupont, S. et al. (2009) Cell 136:123.
  15. Pérez-Mancera, P.A. et al. (2012) Nature 486:266.
  16. Peddaboina, C. et al. (2012) BMC Cancer 12:541.

Long Name

Ubiquitin-specific Protease 9, X-linked

Alternate Names

DFFRX, FAF, FAM

Entrez Gene IDs

8239 (Human); 22284 (Mouse); 363445 (Rat)

Gene Symbol

USP9X

UniProt

NP_001034680

Additional USP9x Products

Product Documents for Recombinant Human His6-USP9x Isoform 2 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-USP9x Isoform 2 Protein, CF

For research use only

Citations for Recombinant Human His6-USP9x Isoform 2 Protein, CF

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FAQs

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail