Recombinant Human His6-USP9x Isoform 2 Protein, CF

Catalog # Availability Size / Price Qty
E-552-025
Product Details
Citations (3)
FAQs
Reviews

Recombinant Human His6-USP9x Isoform 2 Protein, CF Summary

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-USP9x-2 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-USP9x-2 concentration of 0.05-0.5 μM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP9x protein
Contains an N-terminal Met and 6-His tag
Accession #
Predicted Molecular Mass
291 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-552

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 5 mM DTT

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: USP9x

Ubiquitin Specific Peptidase 9, X-linked (USP9x), also known as DFFRX, FAF, and FAM, is a cytoplasmic protein that belongs to the peptidase C19 family of deubiquitinating enzymes (1,2). It is 2570 amino acids (aa) in length with a predicted molecular weight of 292.3 kDa. Human USP9x shares 98% aa sequence identity with the mouse and rat orthologs. USP9x contains a catalytic domain located between aa 1531 and 1971 and a Ubiquitin-like domain that is located at aa 873-963 (3). It is widely expressed and has been shown to interact with and deubiquitinate multiple substrates. Some putative targets include beta -Catenin, TOR, Synuclein-alpha, the Ubiquitin ligases (E3s) Itch, MARCH7, and SMURF1, and the MAP kinase kinase kinase ASK1 (4-10). USP9x has also been shown to control the activity of the AMPK-related kinases ARK5 and MARK4 through direct removal of Lys29- and Lys33-linked Ubiquitin chains (3). It has also been shown to promote cell survival by deubiquitinating and stabilizing the anti-apoptotic protein Mcl-1, and to regulate chromosome alignment and segregation during mitosis by deubiquitinating the chromosome passenger protein Survivin (11-13). Additionally, USP9x appears to promote TGF-beta signaling via deubiquitination of mono-ubiquitinated Smad4 (14). USP9x expression has been shown to be correlated with several cancer types (11,15,16). This recombinant human protein contains an N-terminal His6-tag.

References
  1. Jones, M.H. et al. (1996) Hum. Mol. Genet. 5:1695.
  2. Wood, S.A. et al. (1997) Mech. Dev. 63:29.
  3. Al-Hakim, A.K. et al. (2008) Biochem. J. 411:249.
  4. Murray, R.Z. et al. (2004) Mol. Biol. Cell 15:1591.
  5. Mouchantaf, R. et al. (2006) J. Biol. Chem. 281:38738.
  6. Nathan, J.A. et al. (2008) Traffic 9:1130.
  7. Nagai, H. et al. (2009) Mol. Cell 36:805.
  8. Rott, R. et al. (2011) Proc. Natl. Acad. Sci. USA 108:18666.
  9. Agrawal, P. et al. (2012) J. Biol. Chem. 287:21164.
  10. Xie, Y. et al. (2013) J. Biol. Chem. 288:2976.
  11. Schwickart, M. et al. (2010) Nature 463:103.
  12. Trivigno, D. et al. (2012) Neoplasia 14:893.
  13. Vong, Q.P. et al. (2005) Science 310:1499.
  14. Dupont, S. et al. (2009) Cell 136:123.
  15. Pérez-Mancera, P.A. et al. (2012) Nature 486:266.
  16. Peddaboina, C. et al. (2012) BMC Cancer 12:541.
Long Name
Ubiquitin-specific Protease 9, X-linked
Entrez Gene IDs
8239 (Human); 22284 (Mouse); 363445 (Rat)
Alternate Names
Deubiquitinating enzyme FAF-X; DFFRX; DFFRXubiquitin specific protease 9, X-linked (fat facets-like, Drosophila); Drosophila fat facets related, X-linked; EC 3.1.2.15; EC 3.4.19.12; FAF; FAM; Fat facets in mammals; fat facets protein related, X-linked; Fat facets protein-related, X-linked; hFAM; probable ubiquitin carboxyl-terminal hydrolase FAF-X; ubiquitin specific peptidase 9, X-linked (fat facets-like, Drosophila); ubiquitin specific peptidase 9, X-linked; ubiquitin thioesterase FAF-X; Ubiquitin thiolesterase FAF-X; ubiquitin-specific processing protease FAF-X; Ubiquitin-specific protease 9, X chromosome; Ubiquitin-specific-processing protease FAF-X; USP9; USP9x; X chromosome (fat facets-like Drosophila)

Citations for Recombinant Human His6-USP9x Isoform 2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The deubiquitinase USP9X regulates FBW7 stability and suppresses colorectal cancer
    Authors: OM Khan, J Carvalho, B Spencer-De, R Mitter, D Frith, AP Snijders, SA Wood, A Behrens
    J. Clin. Invest., 2018;0(0):.
    Applications: Bioassay
  2. Grb2-Mediated Recruitment of USP9X to LAT Enhances Themis Stability following Thymic Selection
    Authors: A Garreau, G Blaize, J Argenty, N Rouquié, A Tourdès, SA Wood, A Saoudi, R Lesourne
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  3. Grb2-Mediated Recruitment of USP9X to LAT Enhances Themis Stability following Thymic Selection
    Authors: A Garreau, G Blaize, J Argenty, N Rouquié, A Tourdès, SA Wood, A Saoudi, R Lesourne
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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