Recombinant Human His6UBE2S Protein, CF

R&D Systems | Catalog # E2-690

Will be discontinued when existing inventory is gone.
R&D Systems

Key Product Details

Source

E. coli

Accession Number

NP_055316.2

Applications

Enzyme Activity
View all UBE2S Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human UBE2S protein
Met1 - Leu222 with a N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

26 kDa

Activity

Recombinant Human His6-UBE2S is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2S concentration of 0.1-1 μM.

Formulation, Preparation, and Storage

E2-690
Formulation Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: UBE2S

Ubiquitin-conjugating Enzyme E2S (UBE2S), also known as E2-EPF, is a widely expressed protein with a predicted molecular weight of 26 kDa (1). Human UBE2S shares 92% and 93% amino acid sequence identity with mouse and rat UBE2S, respectively. UBE2S expression oscillates in a cell cycle-dependent fashion, and it influences cell cycle progression by mediating the Ubiquitin-dependent proteolysis of mitotic regulators (2-4). UBE2S interacts with APC/C, a Ubiquitin ligase (E3), to catalyze the elongation of Lys11-linked Ubiquitin chains on APC/C substrates that have been mono-ubiquitinated by UbcH10/UBE2C or UbcH5/UBE2D (3-7). UBE2S is overexpressed in multiple human cancers and targets the tumor suppressor pVHL for proteasomal degradation, which stabilizes HIF-1 alpha and promotes tumor progression (8).

References

  1. Liu, Z. et al. (1992) J. Biol. Chem. 267:15829.
  2. Tedesco, D. et al. (2007) Neoplasia 9:601.
  3. Williamson, A. et al. (2009) Proc. Natl. Acad. Sci. USA 106:18213.
  4. Dimova, N.V. et al. (2012) Nat. Cell Biol. 14:168.
  5. Garnett, M.J. et al. (2009) Nat. Cell Biol. 11:1363.
  6. Wu, T. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1355.
  7. Bremm, A. & D. Komander (2012) Methods Mol. Biol. 832:219.
  8. Jung, C.R. et al. (2006) Nat. Med. 12:809.

Long Name

Ubiquitin-conjugating Enzyme E2S

Alternate Names

E2-24K, E2-EPF, E2-EPF52, EPF5

Entrez Gene IDs

27338 (Human); 292588 (Rat)

Gene Symbol

UBE2S

UniProt

NP_055316.2

Additional UBE2S Products

Product Documents for Recombinant Human His6UBE2S Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6UBE2S Protein, CF

For research use only

Citations for Recombinant Human His6UBE2S Protein, CF

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail