Recombinant Human His6UBE2S Protein, CF
Recombinant Human His6UBE2S Protein, CF Summary
Contains an N-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
X mg/ml (x μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Ubiquitin-conjugating Enzyme E2S (UBE2S), also known as E2-EPF, is a widely expressed protein with a predicted molecular weight of 26 kDa (1). Human UBE2S shares 92% and 93% amino acid sequence identity with mouse and rat UBE2S, respectively. UBE2S expression oscillates in a cell cycle-dependent fashion, and it influences cell cycle progression by mediating the Ubiquitin-dependent proteolysis of mitotic regulators (2-4). UBE2S interacts with APC/C, a Ubiquitin ligase (E3), to catalyze the elongation of Lys11-linked Ubiquitin chains on APC/C substrates that have been mono-ubiquitinated by UbcH10/UBE2C or UbcH5/UBE2D (3-7). UBE2S is overexpressed in multiple human cancers and targets the tumor suppressor pVHL for proteasomal degradation, which stabilizes HIF-1 alpha and promotes tumor progression (8).
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Jung, C.R. et al. (2006) Nat. Med. 12:809.
- Chen M.F., et al. (2009) J. Mol. Med. 87:307-320
- Jung C.-R., et al. (2006) Nature Med. 12:809-816
- Liu Z., et al. (1992) J. Biol. Chem. 267:15829-15835
- Lim J.H., et al. (2008) J. Cell. Biochem. 105:1117-1127
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Citations for Recombinant Human His6UBE2S Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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piRNA-independent function of PIWIL1 as a co-activator for anaphase promoting complex/cyclosome to drive pancreatic cancer metastasis
Authors: F Li, P Yuan, M Rao, CH Jin, W Tang, YF Rong, YP Hu, F Zhang, T Wei, Q Yin, T Liang, L Wu, J Li, D Li, Y Liu, W Lou, S Zhao, MF Liu
Nat. Cell Biol., 2020;0(0):.
Sample Types: Recombinant Protein
Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase
Authors: M Fottner, AD Brunner, V Bittl, D Horn-Ghetk, A Jussupow, VRI Kaila, A Bremm, K Lang
Nat. Chem. Biol., 2019;15(3):276-284.
Sample Types: Recombinant Protein
Applications: Enzyme Assay
Neddylation E2 UBE2F promotes the survival of lung cancer cells by activating CRL5 to degrade NOXA via the K11 linkage
Clin Cancer Res, 2016;0(0):.
Sample Types: Protein
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