Recombinant Human His8-USP7 Protein, CF

R&D Systems | Catalog # E-519

R&D Systems
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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human His8-USP7 Protein (E-519)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

Q93009.2

Applications

Enzyme Activity
View all USP7 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP7 protein
Met1 - Asn1102 with a N-terminal 8-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

130 kDa

Activity

Recombinant Human His8-USP7 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His8-USP7 concentration of 1-5 nM.

Reviewed Applications

Read 3 reviews rated 4.7 using E-519 in the following applications:

Formulation, Preparation, and Storage

E-519
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, EDTA, DTT, and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP7

Ubiquitin Specific Peptidase 7 (USP7), also known as Herpes Virus-associated Ubiquitin Specific Protease (HAUSP), is a widely expressed deubiquitinating enzyme belonging to the peptidase C19 family (1). It has a predicted molecular weight of 130 kDa (2). Human USP7 is 1102 amino acids (aa) in length and shares 99% aa sequence identity with the mouse and rat orthologs (2,3). USP7 consists of a cysteine peptidase core (aa 208-560) that is flanked by an N-terminal TRAF-like domain (aa 50-205) and two C-terminal protease-resistant domains (aa 622-801 and 885-1061) (2,3). USP7 can be phosphorylated at Ser18 and Ser963 and ubiquitinated at Lys869 (2,4). USP7 was initially identified as a p53-interacting protein that deubiquitinates p53, thereby stabilizing the protein and inducing p53-dependent cell growth arrest and apoptosis (5). USP7 also targets the p53 regulatory proteins MDM2, MDMX, and Daxx, the epigenetic regulator Histone 2B, and the transcription factor FoxO4 (4,6-10). Additionally, USP7 interacts with the HSV-1 immediate early protein ICP0, contributing to the stabilization and transactivation capability of ICP0 during HSV-1 infection (1,11,12).

References

  1. Everett, R.D. et al. (1997) EMBO J. 16:1519.
  2. Fernández-Montalván, A. et al. (2007) FEBS J. 274:4256.
  3. Holowaty, M.N. et al. (2003) J. Biol. Chem. 278:47753.
  4. Khoronenkova, S.V. et al. (2012) Mol. Cell 45:801.
  5. Li, M. et al. (2002) Nature 416:648.
  6. Brooks, C.L. & W. Gu (2004) Cell Cycle 3:895.
  7. Meulmeester, E. et al. (2005) Cell Cycle 4:1166.
  8. Tanga, J. et al. (2010) Biochem. Biophys. Res. Commun. 393:542.
  9. van der Knaap, J.A. et al. (2005) Mol. Cell 17:695.
  10. van der Horst, A. et al. (2006) Nat. Cell Biol. 8:1043.
  11. Everett, R.D. et al. (1999) J. Virol. 73:417.
  12. Boutell, C. et al. (2005) J. Virol. 79:12342.

Long Name

Ubiquitin Specific Protease 7

Alternate Names

HAUSP, TEF1

Entrez Gene IDs

7874 (Human); 252870 (Mouse); 360471 (Rat)

Gene Symbol

USP7

UniProt

Q93009.2

Additional USP7 Products

Product Documents for Recombinant Human His8-USP7 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human His8-USP7 Protein, CF

For research use only

Citations for Recombinant Human His8-USP7 Protein, CF

Customer Reviews for Recombinant Human His8-USP7 Protein, CF (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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1 Stars
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  • Recombinant Human His6-USP7 Protein, CF
    Name: Tao An
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 08/26/2019
  • Recombinant Human His6-USP7 Protein, CF
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 04/29/2019
    Recombinant Human His8-USP7 Protein, CF E-519
  • Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 12/05/2018

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