Recombinant Human HSP47 His-tag Protein, CF

Catalog # Availability Size / Price Qty
9166-SH-050
Recombinant Human HSP47 His-tag Protein, CF SDS-PAGE
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Recombinant Human HSP47 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human HSP47, immobilized at 5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.   
Source
Chinese Hamster Ovary cell line, CHO-derived human HSP47 protein
Ala19-Asp412, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala19
Predicted Molecular Mass
44 kDa
SDS-PAGE
47-51 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9166-SH

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human HSP47 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing a band at ~50 kDa under R conditions.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSP47

Heat Shock Protein 47 (HSP47), also known as Serpin-H1, is a 47 kDa collagen-binding stress protein localized in the endoplasmic reticulum (ER) of collagen-secreting cells (1). It is encoded by the SERPINH1 gene, belongs to the serpin family, and contains the typical serpin fold but has no serine protease inhibitory activity (1). Human HSP47 shares 96% aa sequence identity with mouse HSP47. HSP47 is a chaperone that specifically binds pro-collagens at a neutral pH via Gly-Xaa-Arg repeats located on triple-helical procollagen to prevent aggregate formation (2). HSP47 is required for correct procollagen folding in the ER (1). Expression of HSP47 is directly correlated with expression of collagens in multiple types of cells and tissues. Gene disruption of HSP47 in mice causes embryonic lethality due to defects in collagen biosynthesis (1, 3) and HSP47-knockout cells show abnormal helix formation resulting in thin, branched fibrils (1, 4). HSP47 is associated with collagen-related disorders such as osteogenesis imperfecta (5) and fibrosis of the liver, lung, and other organs (6, 7). Its role in collagen regulation, as well as its ability to cause ER stress-mediated apoptosis in collagen-producing cells (6), make HSP47 a promising target for treatment in collagen-related diseases (7, 8). Additionally, HSP47 modulates the tumor microenvironment (9) and may serve as a predictive marker in patients with colorectal, renal, and laryngeal squamous cell carcinomas (10-12).

References
  1. Ito S. and Nagata K. (2017) Seminars in Cell & Developmental Biology. 62:142.
  2. Widmer, C. et al. (2012) Proc. Natl. Am. Soc. 109:13243.
  3. Nagai N. et al. (2000) J. Cell Biol., 150: 1499.
  4. Matsuoka Y. et al. (2004) Mol. Biol. Cell, 15: 4467.
  5. Christiansen, H. E. et al. (2010) Am. J. Hum. Genet. 86:389.
  6. Kawasaki, K. et al. (2015) J. Biol. Chem. 290:3639.
  7. Ito, S. et al. (2017) J. Biol. Chem. 292:20076.
  8. Otsuka, M. et al. (2017) Exp. Lung Res. 43:271.
  9. Jiang X. et al. ACS Chem Neurosci. (2017) 8:128.
  10. Mori K. et al. (2017) Int J Cancer. 140: 1425.
  11. Song, X. et al. (2017) Oncol. Rep. 38:2444.
  12. Qi, Y. et al. (2018) J. Cell Mol. Med. 22:1224.
Long Name
Heat Shock Protein 47
Entrez Gene IDs
871 (Human); 12406 (Mouse); 29345 (Rat)
Alternate Names
(collagen binding protein 1); Arsenic-transactivated protein 3; AsTP3; BERF-1; CBP1; CBP2; CBP2RA-A47; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; colligen; Colligin; colligin-1; colligin-2; gp46; HSP47; HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47); member 1, (collagen binding protein 1); member 2; member 2, (collagen-binding protein 2); OI10; PPROM; proliferation-inducing gene 14; RA-A47; rheumatoid arthritis antigen A-47,47 kDa heat shock protein; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47); Serpin H1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1; SERPINH1; SERPINH2colligin

FAQs

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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