Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
720-IC-050
720-IC-200
Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF SDS-PAGE
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Citations (44)
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Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to recombinant human ICAM Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >60% will adhere after PMA 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human ICAM-1/CD54 protein
Human ICAM-1
Gln28-Glu480
Accession # CAA30051
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
76 kDa (monomer)
SDS-PAGE
110-125 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

720-IC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 12 weeks, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE View Larger

1 μg/lane of Recombinant Human ICAM-1/CD54 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 110 kDa and 190 kDa, respectively.

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Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

References
  1. Pigott, R. and C. Power, 1993, The Adhesion Molecule Facts Book, pp. 74. Academic Press.
Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

Citations for Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Adhesion of T cells to endothelial cells facilitates blinatumomab-associated neurologic adverse events
    Authors: M Klinger, G Zugmaier, V Nägele, ME Goebeler, C Brandl, M Stelljes, H Lassmann, A von Stacke, RC Bargou, P Kufer
    Cancer Res., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The Extracellular Domain of the β2 Integrin β Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity
    Authors: LC Ristow, V Tran, KJ Schwartz, L Pankratz, A Mehle, JD Sauer, RA Welch
    MBio, 2019;10(4):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  3. Frontline Science: A flexible kink in the transmembrane domain impairs β2 integrin extension and cell arrest from rolling
    Authors: H Sun, Z Fan, AR Gingras, MA Lopez-Rami, MH Ginsberg, K Ley
    J. Leukoc. Biol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Biomimetic post-capillary venule expansions for leukocyte adhesion studies
    Authors: BL Benson, L Li, JT Myers, RD Dorand, UA Gurkan, AY Huang, RM Ransohoff
    Sci Rep, 2018;8(1):9328.
  5. ZAP70 expression enhances chemokine-driven chronic lymphocytic leukemia cell migration and arrest by valency regulation of integrins
    Authors: JM Laufer, R Lyck, DF Legler
    FASEB J., 2018;0(0):fj201701452RR.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Cis interaction between sialylated Fc?RIIA and the ?I-domain of Mac-1 limits antibody-mediated neutrophil recruitment
    Authors: G Saggu, K Okubo, Y Chen, R Vattepu, N Tsuboi, F Rosetti, X Cullere, N Washburn, S Tahir, AM Rosado, SM Holland, RM Anthony, M Sen, C Zhu, TN Mayadas
    Nat Commun, 2018;9(1):5058.
  7. Live nanoscopic to mesoscopic topography reconstruction with an optical microscope for chemical and biological samples
    Authors: O Theodoly, N Garcia-Sey, F Bedu, X Luo, S Gabriele, T Mignot, J Giermanska, JP Chapel, M Métivier, MP Valignat
    PLoS ONE, 2018;13(12):e0207881.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. The Wiskott-Aldrich Syndrome Protein Contributes to the Assembly of the LFA-1 Nanocluster Belt at the Lytic Synapse
    Authors: R Houmadi, D Guipouy, J Rey-Barros, Z Vasconcelo, J Cornet, M Manghi, N Destainvil, S Valitutti, S Allart, L Dupré
    Cell Rep, 2018;22(4):979-991.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. A Developed NK-92MI Cell Line with Siglec-7negPhenotype Exhibits High and Sustainable Cytotoxicity against Leukemia Cells
    Authors: CH Huang, YJ Liao, TH Fan, TJ Chiou, YH Lin, YC Twu
    Int J Mol Sci, 2018;19(4):.
    Applications: Bioassay
  10. Phosphorylation of the ?-chain in the integrin LFA-1 enables ?2-chain phosphorylation and ?-actinin binding required for cell adhesion
    Authors: F Jahan, S Madhavan, T Rolova, L Viazmina, M Grönholm, CG Gahmberg
    J. Biol. Chem., 2018;0(0):.
    Applications: Bioassay
  11. Changes in clinical laboratory parameters and pharmacodynamic markers in response to blinatumomab treatment of patients with relapsed/refractory ALL
    Authors: V Nägele, A Kratzer, G Zugmaier, C Holland, Y Hijazi, MS Topp, N Gökbuget, PA Baeuerle, P Kufer, A Wolf, M Klinger
    Exp Hematol Oncol, 2017;6(0):14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. P-Selectin and ICAM-1 synergy in mediating THP-1 monocyte adhesion in hemodynamic flow is length dependent
    Authors: EE Edwards, SN Thomas
    Integr Biol (Camb), 2017;9(4):313-327.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. PKN1 Directs Polarized RAB21 Vesicle Trafficking via RPH3A and Is Important for Neutrophil Adhesion and Ischemia-Reperfusion Injury
    Authors: Q Yuan, C Ren, W Xu, B Petri, J Zhang, Y Zhang, P Kubes, D Wu, W Tang
    Cell Rep, 2017;19(12):2586-2597.
    Species: Goat
    Sample Types: Antibody
    Applications: Bioassay
  14. T-Time: A data repository of T cell and calcium release-activated calcium channel activation imagery
    Authors: C Arbuckle, M Greenberg, A Bergh, R German, N Sirago, E Linstead
    BMC Res Notes, 2017;10(1):408.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. A human immunodeficiency syndrome caused by mutations in CARMIL2
    Authors: T Schober, T Magg, M Laschinger, M Rohlfs, ND Linhares, J Puchalka, T Weisser, K Fehlner, J Mautner, C Walz, K Hussein, G Jaeger, B Kammer, I Schmid, M Bahia, SD Pena, U Behrends, BH Belohradsk, C Klein, F Hauck
    Nat Commun, 2017;8(0):14209.
    Species: Human
    Sample Types: Whole Cells
    Applications: Migration
  16. Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial Cells
    MBio, 2016;7(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. The evolution of multivalent nanoparticle adhesion via specific molecular interactions
    Authors: Jered Brackston Haun
    Langmuir, 2016;0(0):.
    Applications: Bioassay
  18. Recruitment of calcineurin to the TCR positively regulates T cell activation
    Nat. Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Identification of pathogenic factors potentially involved in Staphylococcus aureus keratitis using proteomics
    Exp Eye Res, 2016;151(0):171-178.
    Species: Bacteria - Staphylococcus aureus
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Calcium influx through CRAC channels controls actin organization and dynamics at the immune synapse
    Elife, 2016;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. ARAP, a Novel Adaptor Protein, Is Required for TCR Signaling and Integrin-Mediated Adhesion
    Authors: Seung Hee Jung
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. WNK1 kinase balances T cell adhesion versus migration in vivo
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo.
    Authors: Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E
    Nat Commun, 2015;6(0):6387.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Enhanced Chemokine Receptor Recycling and Impaired S1P1 Expression Promote Leukemic Cell Infiltration of Lymph Nodes in Chronic Lymphocytic Leukemia.
    Authors: Patrussi L, Capitani N, Martini V, Pizzi M, Trimarco V, Frezzato F, Marino F, Semenzato G, Trentin L, Baldari C
    Cancer Res, 2015;75(19):4153-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Multi-Inhibitory Effects of A2A Adenosine Receptor Signaling on Neutrophil Adhesion Under Flow.
    Authors: Yago T, Tsukamoto H, Liu Z, Wang Y, Thompson L, McEver R
    J Immunol, 2015;195(8):3880-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse.
    Authors: Na B, Kim H, Piragyte I, Oh H, Kwon M, Akber U, Lee H, Park D, Song W, Park Z, Im S, Rho M, Hyun Y, Kim M, Jun C
    J Cell Biol, 2015;209(1):143-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Adhesion Assay
  27. The Neurorepellent Slit2 Inhibits Postadhesion Stabilization of Monocytes Tethered to Vascular Endothelial Cells.
    Authors: Mukovozov I, Huang Y, Zhang Q, Liu G, Siu A, Sokolskyy Y, Patel S, Hyduk S, Kutryk M, Cybulsky M, Robinson L
    J Immunol, 2015;195(7):3334-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. B-Raf regulation of integrin alpha4beta1-mediated resistance to shear stress through changes in cell spreading and cytoskeletal association in T cells.
    Authors: Brown W, Khalili J, Rodriguez-Cruz T, Lizee G, McIntyre B
    J Biol Chem, 2014;289(33):23141-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. ICAM-2 facilitates luminal interactions between neutrophils and endothelial cells in vivo.
    Authors: Halai, Krishma, Whiteford, James, Ma, Bin, Nourshargh, Sussan, Woodfin, Abigail
    J Cell Sci, 2014;127(0):620-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Phosphorylation of Rab5a protein by protein kinase C is crucial for T-cell migration.
    Authors: Ong S, Freeley M, Skubis-Zegadlo J, Fazil M, Kelleher D, Fresser F, Baier G, Verma N, Long A
    J Biol Chem, 2014;289(28):19420-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Toxoplasma gondii modulates the dynamics of human monocyte adhesion to vascular endothelium under fluidic shear stress.
    Authors: Harker K, Ueno N, Wang T, Bonhomme C, Liu W, Lodoen M
    J Leukoc Biol, 2013;93(5):789-800.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Selective inhibition of KCa3.1 channels mediates adenosine regulation of the motility of human T cells.
    Authors: Chimote, Ameet A, Hajdu, Peter, Kucher, Vladimir, Boiko, Nina, Kuras, Zerrin, Szilagyi, Orsolya, Yun, Yeo-Heun, Conforti, Laura
    J Immunol, 2013;191(12):6273-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Modulation of integrin activation and signaling by alpha1/alpha1'-helix unbending at the junction.
    Authors: Zhang C, Liu J, Jiang X, Haydar N, Zhang C, Shan H, Zhu J
    J Cell Sci, 2013;126(0):5735-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation.
    Authors: Li N, Mao D, Lu S, Tong C, Zhang Y, Long M
    J Immunol, 2013;190(8):4371-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction induces a novel genetic signature resulting in T-cells refractory to transforming growth factor-beta signaling.
    Authors: Verma N, Dempsey E, Long A, Davies A, Barry S, Fallon P, Volkov Y, Kelleher D
    J Biol Chem, 2012;287(32):27204-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Wiskott-Aldrich syndrome protein (WASp) and N-WASp are involved in the regulation of NK-cell migration upon NKG2D activation.
    Eur J Immunol, 2012;42(8):2142-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Epstein-Barr virus infection of polarized epithelial cells via the basolateral surface by memory B cell-mediated transfer infection.
    Authors: Shannon-Lowe C, Rowe M
    PLoS Pathog., 2011;7(5):e1001338.
    Species: Human
    Sample Types: Whole Cells
    Applications: Adhesion Assay
  38. CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients.
    Authors: Martinez-Vinambres E, Garcia-Trujillo JA, Rodriguez-Martin E, Villar LM, Coll J, Roldan E
    Leukemia, 2011;26(5):1098-105.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. A mechanism for antibody-mediated outside-in activation of LFA-1.
    Authors: Carreno R, Li D, Sen M, Nira I, Yamakawa T, Ma Q, Legge GB
    J. Biol. Chem., 2008;283(16):10642-8.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  40. JAM-L-mediated leukocyte adhesion to endothelial cells is regulated in cis by alpha4beta1 integrin activation.
    Authors: Luissint AC, Lutz PG, Calderwood DA, Couraud PO, Bourdoulous S
    J. Cell Biol., 2008;183(6):1159-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces.
    Authors: Woolf E, Grigorova I, Sagiv A, Grabovsky V, Feigelson SW, Shulman Z, Hartmann T, Sixt M, Cyster JG, Alon R
    Nat. Immunol., 2007;8(10):1076-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Activation of CR3-mediated phagocytosis by MSP requires the RON receptor, tyrosine kinase activity, phosphatidylinositol 3-kinase, and protein kinase C zeta.
    Authors: Lutz MA, Correll PH
    J. Leukoc. Biol., 2003;73(6):802-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  43. CCR6 colocalizes with CD18 and enhances adhesion to activated endothelial cells in CCR6-transduced Jurkat T cells.
    Authors: Maki W, Morales RE, Carroll VA, Telford WG, Knibbs RN, Stoolman LM, Hwang ST
    J. Immunol., 2002;169(5):2346-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Homeostatic regulation of T cell trafficking by a B cell-derived peptide is impaired in autoimmune and chronic inflammatory disease.
    Authors: Chimen M, McGettrick H, Apta B, Kuravi S, Yates C, Kennedy A, Odedra A, Alassiri M, Harrison M, Martin A, Barone F, Nayar S, Hitchcock J, Cunningham A, Raza K, Filer A, Copland D, Dick A, Robinson J, Kalia N, Walker L, Buckley C, Nash G, Narendran P, Rainger G
    Nat Med, 0;21(5):467-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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