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Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

R&D Systems | Catalog # 720-IC

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human ICAM-1/CD54 Fc Chimera Protein (720-IC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

CAA30051

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all ICAM-1/CD54 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ICAM-1/CD54 protein
Human ICAM-1
Gln28-Glu480
Accession # CAA30051		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln28 predicted

Predicted Molecular Mass

76 kDa (monomer)

SDS-PAGE

110-125 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When 5 x 104 cells/well are added to recombinant human ICAM Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >60% will adhere after PMA 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.

Reviewed Applications

Read 2 reviews rated 4.5 using 720-IC in the following applications:

Scientific Data Images for Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

Recombinant Human ICAM-1/CD54 Fc Chimera Protein SDS-PAGE

Recombinant Human ICAM-1/CD54 Fc Chimera Protein SDS-PAGE

1 μg/lane of Recombinant Human ICAM-1/CD54 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 110 kDa and 190 kDa, respectively.

Formulation, Preparation, and Storage

720-IC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 400 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 12 weeks, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

References

  1. Pigott, R. and C. Power, 1993, The Adhesion Molecule Facts Book, pp. 74. Academic Press.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Entrez Gene IDs

3383 (Human); 15894 (Mouse); 25464 (Rat)

Gene Symbol

ICAM1

UniProt

CAA30051

Additional ICAM-1/CD54 Products

Product Documents for Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

Customer Reviews for Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF (2)

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  • Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Cell Adhesion
    Verified Customer | Posted 07/01/2022
  • Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 04/30/2021

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