Recombinant Human ICAM-1/CD54 Fc Chimera Protein: R&D Systems

Recombinant Human ICAM-1/CD54 Fc Chimera Protein, CF

(31 citations)   
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to recombinant human ICAM Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >60% will adhere after PMA 1 hour incubation at 37 °C.
    Optimal dilutions should be determined by each laboratory for each application.
  • Source
    Mouse myeloma cell line, NS0-derived
    Human ICAM-1
    Gln28-Glu480
    Accession # CAA30051
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained: Gln28 predicted
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    76 kDa (monomer)
  • SDS-PAGE
    110-125 kDa, reducing conditions
720-IC
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 12 weeks, -20 to -70 °C under sterile conditions after reconstitution.
Data Images

1 μg/lane of Recombinant Human
ICAM-1/CD54 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 110 kDa and 190 kDa, respectively.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

  • References:
    1. Pigott, R. and C. Power, 1993, The Adhesion Molecule Facts Book, pp. 74. Academic Press.
  • Long Name:
    Intercellular Adhesion Molecule 1
  • Entrez Gene IDs:
    3383 (Human); 15894 (Mouse); 25464 (Rat)
  • Alternate Names:
    BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. T-Time: A data repository of T cell and calcium release-activated calcium channel activation imagery
    Authors: C Arbuckle, M Greenberg, A Bergh, R German, N Sirago, E Linstead
    BMC Res Notes, 2017;10(1):408.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. A human immunodeficiency syndrome caused by mutations in CARMIL2
    Authors: T Schober, T Magg, M Laschinger, M Rohlfs, ND Linhares, J Puchalka, T Weisser, K Fehlner, J Mautner, C Walz, K Hussein, G Jaeger, B Kammer, I Schmid, M Bahia, SD Pena, U Behrends, BH Belohradsk, C Klein, F Hauck
    Nat Commun, 2017;8(0):14209.
    Species: Human
    Sample Type: Whole Cells
    Application: Migration assay
  3. PKN1 Directs Polarized RAB21 Vesicle Trafficking via RPH3A and Is Important for Neutrophil Adhesion and Ischemia-Reperfusion Injury
    Authors: Q Yuan, C Ren, W Xu, B Petri, J Zhang, Y Zhang, P Kubes, D Wu, W Tang
    Cell Rep, 2017;19(12):2586-2597.
    Species: Goat
    Sample Type: Antibody
    Application: Bioassay
  4. Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial Cells
    MBio, 2016;7(4):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. WNK1 kinase balances T cell adhesion versus migration in vivo
    Nat Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. ARAP, a Novel Adaptor Protein, Is Required for TCR Signaling and Integrin-Mediated Adhesion
    Authors: Seung Hee Jung
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Calcium influx through CRAC channels controls actin organization and dynamics at the immune synapse
    Elife, 2016;5(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Recruitment of calcineurin to the TCR positively regulates T cell activation
    Nat. Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Identification of pathogenic factors potentially involved in Staphylococcus aureus keratitis using proteomics
    Exp Eye Res, 2016;151(0):171-178.
    Species: Bacteria - Staphylococcus aureus
    Sample Type: Whole Cells
    Application: Bioassay
  10. The Neurorepellent Slit2 Inhibits Postadhesion Stabilization of Monocytes Tethered to Vascular Endothelial Cells.
    Authors: Mukovozov I, Huang Y, Zhang Q, Liu G, Siu A, Sokolskyy Y, Patel S, Hyduk S, Kutryk M, Cybulsky M, Robinson L
    J Immunol, 2015;195(7):3334-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Enhanced Chemokine Receptor Recycling and Impaired S1P1 Expression Promote Leukemic Cell Infiltration of Lymph Nodes in Chronic Lymphocytic Leukemia.
    Authors: Patrussi L, Capitani N, Martini V, Pizzi M, Trimarco V, Frezzato F, Marino F, Semenzato G, Trentin L, Baldari C
    Cancer Res, 2015;75(19):4153-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo.
    Authors: Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E
    Nat Commun, 2015;6(0):6387.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Multi-Inhibitory Effects of A2A Adenosine Receptor Signaling on Neutrophil Adhesion Under Flow.
    Authors: Yago T, Tsukamoto H, Liu Z, Wang Y, Thompson L, McEver R
    J Immunol, 2015;195(8):3880-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse.
    Authors: Na B, Kim H, Piragyte I, Oh H, Kwon M, Akber U, Lee H, Park D, Song W, Park Z, Im S, Rho M, Hyun Y, Kim M, Jun C
    J Cell Biol, 2015;209(1):143-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell Adhesion
  15. B-Raf regulation of integrin alpha4beta1-mediated resistance to shear stress through changes in cell spreading and cytoskeletal association in T cells.
    Authors: Brown W, Khalili J, Rodriguez-Cruz T, Lizee G, McIntyre B
    J Biol Chem, 2014;289(33):23141-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Phosphorylation of Rab5a protein by protein kinase C is crucial for T-cell migration.
    Authors: Ong S, Freeley M, Skubis-Zegadlo J, Fazil M, Kelleher D, Fresser F, Baier G, Verma N, Long A
    J Biol Chem, 2014;289(28):19420-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. ICAM-2 facilitates luminal interactions between neutrophils and endothelial cells in vivo.
    Authors: Halai, Krishma, Whiteford, James, Ma, Bin, Nourshargh, Sussan, Woodfin, Abigail
    J Cell Sci, 2014;127(0):620-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Toxoplasma gondii modulates the dynamics of human monocyte adhesion to vascular endothelium under fluidic shear stress.
    Authors: Harker K, Ueno N, Wang T, Bonhomme C, Liu W, Lodoen M
    J Leukoc Biol, 2013;93(5):789-800.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation.
    Authors: Li N, Mao D, Lu S, Tong C, Zhang Y, Long M
    J Immunol, 2013;190(8):4371-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. Modulation of integrin activation and signaling by alpha1/alpha1'-helix unbending at the junction.
    Authors: Zhang C, Liu J, Jiang X, Haydar N, Zhang C, Shan H, Zhu J
    J Cell Sci, 2013;126(0):5735-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  21. Selective inhibition of KCa3.1 channels mediates adenosine regulation of the motility of human T cells.
    Authors: Chimote, Ameet A, Hajdu, Peter, Kucher, Vladimir, Boiko, Nina, Kuras, Zerrin, Szilagyi, Orsolya, Yun, Yeo-Heun, Conforti, Laura
    J Immunol, 2013;191(12):6273-80.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction induces a novel genetic signature resulting in T-cells refractory to transforming growth factor-beta signaling.
    Authors: Verma N, Dempsey E, Long A, Davies A, Barry S, Fallon P, Volkov Y, Kelleher D
    J Biol Chem, 2012;287(32):27204-16.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. Wiskott-Aldrich syndrome protein (WASp) and N-WASp are involved in the regulation of NK-cell migration upon NKG2D activation.
    Eur J Immunol, 2012;42(8):2142-51.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients.
    Authors: Martinez-Vinambres E, Garcia-Trujillo JA, Rodriguez-Martin E, Villar LM, Coll J, Roldan E
    Leukemia, 2011;26(5):1098-105.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. Epstein-Barr virus infection of polarized epithelial cells via the basolateral surface by memory B cell-mediated transfer infection.
    Authors: Shannon-Lowe C, Rowe M
    PLoS Pathog., 2011;7(5):e1001338.
    Species: Human
    Sample Type: Whole Cells
    Application: Adhesion Assay
  26. A mechanism for antibody-mediated outside-in activation of LFA-1.
    Authors: Carreno R, Li D, Sen M, Nira I, Yamakawa T, Ma Q, Legge GB
    J. Biol. Chem., 2008;283(16):10642-8.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Surface Plasmon Resonance
  27. JAM-L-mediated leukocyte adhesion to endothelial cells is regulated in cis by alpha4beta1 integrin activation.
    Authors: Luissint AC, Lutz PG, Calderwood DA, Couraud PO, Bourdoulous S
    J. Cell Biol., 2008;183(6):1159-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  28. Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces.
    Authors: Woolf E, Grigorova I, Sagiv A, Grabovsky V, Feigelson SW, Shulman Z, Hartmann T, Sixt M, Cyster JG, Alon R
    Nat. Immunol., 2007;8(10):1076-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. Activation of CR3-mediated phagocytosis by MSP requires the RON receptor, tyrosine kinase activity, phosphatidylinositol 3-kinase, and protein kinase C zeta.
    Authors: Lutz MA, Correll PH
    J. Leukoc. Biol., 2003;73(6):802-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  30. CCR6 colocalizes with CD18 and enhances adhesion to activated endothelial cells in CCR6-transduced Jurkat T cells.
    Authors: Maki W, Morales RE, Carroll VA, Telford WG, Knibbs RN, Stoolman LM, Hwang ST
    J. Immunol., 2002;169(5):2346-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  31. Homeostatic regulation of T cell trafficking by a B cell-derived peptide is impaired in autoimmune and chronic inflammatory disease.
    Authors: Chimen M, McGettrick H, Apta B, Kuravi S, Yates C, Kennedy A, Odedra A, Alassiri M, Harrison M, Martin A, Barone F, Nayar S, Hitchcock J, Cunningham A, Raza K, Filer A, Copland D, Dick A, Robinson J, Kalia N, Walker L, Buckley C, Nash G, Narendran P, Rainger G
    Nat Med, 0;21(5):467-75.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 31 Citations
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