Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.
Recombinant Human ICAM-1/CD54 Protein, CF
R&D Systems | Catalog # ADP4
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Key Product Details
- R&D Systems NS0-derived Recombinant Human ICAM-1/CD54 Protein (ADP4)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Applications
Bioactivity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ICAM-1/CD54 protein
Met1-Glu480
Met1-Glu480
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Not obtained, Predicted Gln28
Predicted Molecular Mass
50 kDa
SDS-PAGE
70-90 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When 5 x 104 cells/well are added to Recombinant Human ICAM‑1/CD54 coated plates (12.5 μg/mL with 100 μL/well), >30% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
When 5 x 104 cells/well are added to Recombinant Human ICAM‑1/CD54 coated plates (12.5 μg/mL with 100 μL/well), >30% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Reviewed Applications
Read 1 review rated 5 using ADP4 in the following applications:
Formulation, Preparation, and Storage
ADP4
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, containing Calcium and Magnesium with Sorbitol and with Trehalose. |
| Reconstitution | Reconstitute at 400 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: ICAM-1/CD54
Long Name
Intercellular Adhesion Molecule 1
Alternate Names
CD54, ICAM1
Gene Symbol
ICAM1
UniProt
Additional ICAM-1/CD54 Products
Product Documents for Recombinant Human ICAM-1/CD54 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human ICAM-1/CD54 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human ICAM-1/CD54 Protein, CF
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Customer Reviews for Recombinant Human ICAM-1/CD54 Protein, CF (1)
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1 Customer Rating
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Application: Immunoassay StandardVerified Customer | Posted 11/06/2018The recombinant ICAM protein worked well as a calibrator in an ELSIA built to measure ICAM in human plasma samples.
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