Recombinant Human ICAM-1/CD54 Protein, CF

Catalog # Availability Size / Price Qty
ADP4-050
ADP4-200
Product Details
Citations (25)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human ICAM-1/CD54 Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to Recombinant Human ICAM‑1/CD54 coated plates (12.5 μg/mL with 100 μL/well), >30% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human ICAM-1/CD54 protein
Met1-Glu480
Accession #
N-terminal Sequence
Analysis
Not obtained, Predicted Gln28
Predicted Molecular Mass
50 kDa
SDS-PAGE
70-90 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ADP4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, containing Calcium and Magnesium with Sorbitol and with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

Citations for Recombinant Human ICAM-1/CD54 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3)
    Authors: F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli
    Int J Mol Sci, 2019;20(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
    Authors: NV Ayala-Nune, G Follain, F Delalande, A Hirschler, E Partiot, GL Hale, BC Bollweg, J Roels, M Chazal, F Bakoa, M Carocci, S Bourdoulou, O Faklaris, SR Zaki, A Eckly, B Uring-Lamb, F Doussau, S Cianferani, C Carapito, FMJ Jacobs, N Jouvenet, JG Goetz, R Gaudin
    Nat Commun, 2019;10(1):4430.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. In Vivo Near-Infrared Fluorescence Imaging of Atherosclerosis Using Local Delivery of Novel Targeted Molecular Probes
    Authors: MJ Bertrand, M Abran, F Maafi, D Busseuil, N Merlet, T Mihalache-, P Geoffroy, PL Tardif, A Abulrob, M Arbabi-Gha, F Ni, M Sirois, PL L'Allier, É Rhéaume, F Lesage, JC Tardif
    Sci Rep, 2019;9(1):2670.
    Species: Human
    Sample Types:
    Applications: Affinity Purification
  4. A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense
    Authors: D Wolf, N Anto-Miche, H Blankenbac, A Wiedemann, K Buscher, JD Hohmann, B Lim, M Bäuml, A Marki, M Mauler, D Duerschmie, Z Fan, H Winkels, D Sidler, P Diehl, DM Zajonc, I Hilgendorf, P Stachon, T Marchini, F Willecke, M Schell, B Sommer, C von Zur Mu, J Reinöhl, T Gerhardt, EF Plow, V Yakubenko, P Libby, C Bode, K Ley, K Peter, A Zirlik
    Nat Commun, 2018;9(1):525.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. An actin cytoskeletal barrier inhibits lytic granule release from natural killer cells in patients with Chediak-Higashi syndrome
    Authors: A Gil-Krzews, MB Saeed, A Oszmiana, ER Fischer, K Lagrue, WA Gahl, WJ Introne, JE Coligan, DM Davis, K Krzewski
    J. Allergy Clin. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. The protective activity of mesothelial cells against peritoneal growth of gastrointestinal tumors: The role of soluble ICAM-1
    Authors: J Miku?a-Pie, P Uruski, M Kuci?ska, A Tykarski, K Ksi??ek
    Int. J. Biochem. Cell Biol, 2017;86(0):26-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Human Melanoma-Derived Extracellular Vesicles Regulate Dendritic Cell Maturation
    Authors: RLG Maus, JW Jakub, WK Nevala, TA Christense, K Noble-Orcu, Z Sachs, TJ Hieken, SN Markovic
    Front Immunol, 2017;8(0):358.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. A Rational Engineering Strategy for Designing Protein A-Binding Camelid Single-Domain Antibodies
    PLoS ONE, 2016;11(9):e0163113.
    Applications: Surface Plasmon Resonance
  9. Structural basis for simvastatin competitive antagonism of complement receptor 3
    Authors: Maria Risager Jensen
    J Biol Chem, 2016;0(0):.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  10. Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases
    Cell Death Dis, 2016;7(12):e2565.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Lenalidomide augments actin remodeling and lowers NK-cell activation thresholds.
    Authors: Lagrue K, Carisey A, Morgan D, Chopra R, Davis D
    Blood, 2015;126(1):50-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Microparticle alpha-2-macroglobulin enhances pro-resolving responses and promotes survival in sepsis.
    Authors: Dalli, Jesmond, Norling, Lucy V, Montero-Melendez, Trinidad, Federici Canova, Donata, Lashin, Hazem, Pavlov, Anton M, Sukhorukov, Gleb B, Hinds, Charles, Perretti, Mauro
    EMBO Mol Med, 2014;6(1):27-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via beta2 integrins (CD11/CD18).
    Authors: Weckbach L, Gola A, Winkelmann M, Jakob S, Groesser L, Borgolte J, Pogoda F, Pick R, Pruenster M, Muller-Hocker J, Deindl E, Sperandio M, Walzog B
    Blood, 2014;123(12):1887-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Dynamics in the cytoadherence phenotypes of Plasmodium falciparum infected erythrocytes isolated during pregnancy.
    Authors: Doritchamou J, Sossou-tchatcha S, Cottrell G, Moussiliou A, Hounton Houngbeme C, Massougbodji A, Deloron P, Ndam N
    PLoS ONE, 2014;9(6):e98577.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Priming by chemokines restricts lateral mobility of the adhesion receptor LFA-1 and restores adhesion to ICAM-1 nano-aggregates on human mature dendritic cells.
    Authors: Borgman K, van Zanten T, Manzo C, Cabezon R, Cambi A, Benitez-Ribas D, Garcia-Parajo M
    PLoS ONE, 2014;9(6):e99589.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. The innate defense regulator peptides IDR-HH2, IDR-1002, and IDR-1018 modulate human neutrophil functions.
    Authors: Niyonsaba F, Madera L, Afacan N, Okumura K, Ogawa H, Hancock R
    J Leukoc Biol, 2013;94(1):159-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation.
    Authors: Li N, Mao D, Lu S, Tong C, Zhang Y, Long M
    J Immunol, 2013;190(8):4371-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Super-resolution imaging of remodeled synaptic actin reveals different synergies between NK cell receptors and integrins.
    Authors: Brown A, Dobbie I, Alakoskela J, Davis I, Davis D
    Blood, 2012;120(18):3729-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Complement Receptor Mac-1 Is an Adaptor for NB1 (CD177)-mediated PR3-ANCA Neutrophil Activation.
    Authors: Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, Kettritz R
    J. Biol. Chem., 2011;286(9):7070-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Adhesion Assay
  20. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009;51(4):734-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  22. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  23. Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma.
    Authors: Brooks AM, Bates ME, Vrtis RF, Jarjour NN, Bertics PJ, Sedgwick JB
    Am. J. Respir. Cell Mol. Biol., 2006;35(4):503-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis.
    Authors: Tamai R, Asai Y, Ogawa T
    Infect. Immun., 2005;73(10):6290-8.
    Species: Bacteria
    Sample Types: Bacteria Lysates
    Applications: ELISA (Capture)
  25. CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
    Authors: Yamamoto H, Nagata M, Sakamoto Y
    Ann. Allergy Asthma Immunol., 2005;94(2):292-300.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay

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Reviews for Recombinant Human ICAM-1/CD54 Protein, CF

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Recombinant Human ICAM-1/CD54 Protein, CF
By Anonymous on 11/06/2018
Application: Immunoassay Standard

The recombinant ICAM protein worked well as a calibrator in an ELSIA built to measure ICAM in human plasma samples.