Recombinant Human ICAM-5 Fc Chimera Protein, CF

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Recombinant Human ICAM-5 Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to Recombinant Human ICAM-5/Fc Chimera coated plates (25 µg/mL, 100 µL/well), >25% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived human ICAM-5 protein
Human ICAM-5
Accession # Q9UMF0
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85.1 kDa (monomer)
125-130 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: ICAM-5

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. Human ICAM-5 consists of an 832 amino acid (aa) extracellular domain containing 9 immunoglobulin (Ig) domains and 15 N-glycosylation sites, a 28 aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM-5 shares 38 - 55% aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity) and ICAM-3 (55% identity) (1). Human and mouse ICAM-5 share 85% aa identity.

The tissue distribution of ICAM-5 is unique among ICAMs, being expressed only in telencephalic regions of the central nervous system (2). Like other ICAMs, ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) (3). Binding of ICAM-5 to LFA-1 is dependent on the first amino terminal Ig domain of ICAM-5 (4). ICAM-5 also displays homophilic binding, with the amino terminal Ig domain binding to Ig domains 4 - 5. Homophilic binding of ICAM-5 is dependent of ICAM-5 being in a monomeric form. The monomeric form of ICAM-5 is found during dendritogenesis in developing brain, whereas a high molecular weight complex is found in mature neurons (5).

  1. Mizuno, T. et al. (1997) J. Biol. Chem. 272:1156.
  2. Yoshihara, Y. et al. (1994) Neuron 12:541.
  3. Tain, L. et al. (1997) J. Immunol. 158:928.
  4. Tain, L. et al. (2000) Eur. J. Immunol. 30:810.
  5. Tain, L. et al. (2000) J. Immunol. 150:243.
Long Name
Intercellular Adhesion Molecule 5
Entrez Gene IDs
7087 (Human); 15898 (Mouse)
Alternate Names
ICAM5; ICAM-5; intercellular adhesion molecule 5; intercellular adhesion molecule 5, telencephalin; Telencephalin; TLCNTelencephalin; TLNtelencephalin

Citations for Recombinant Human ICAM-5 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Bypassing pan-enterovirus host factor PLA2G16
    Authors: J Baggen, Y Liu, H Lyoo, ALW van Vliet, M Wahedi, JW de Bruin, RW Roberts, P Overduin, A Meijer, MG Rossmann, HJ Thibaut, FJM van Kuppev
    Nat Commun, 2019;10(1):3171.
    Species: Virus
    Sample Types: Virus
    Applications: Bioassay
  2. ICAM-5/Telencephalin Is a Functional Entry Receptor for Enterovirus D68
    Authors: Wei Wei
    Cell Host Microbe, 2016;20(5):631-641.
    Applications: Bioassay


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