Recombinant Human IFN-lambda 4 Protein, CF 9165-IF-025: R&D Systems

Recombinant Human IFN-lambda 4 Protein, CF

  
  • Purity
    >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED50 for this effect is 0.2-1.2 μg/mL.
  • Source
    E. coli-derived Ala23-Leu179 with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met, Ala23
  • Predicted Molecular Mass
    18 kDa
  • SDS-PAGE
    19 kDa, reducing conditions
9165-IF
 
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human IFN-lambda 4 (Catalog #
9165-IF) exhibits anti-viral activity in HepG2human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC)virus. The ED50 for this effect is 0.2-1.2 μg/mL.
Background: IFN-lambda 4
Interferon-lambda 4 (IFN-lambda 4) is a secreted, approximately 17 kDa member of the type III interferon family of molecules (1). It is generated by a frameshift mutation based on a TT > ∆G polymorphism (2). The TT allele inactivates expression of IFN-lambda 4 (2). The ∆G allele is associated with reduced clearance of hepatitis C virus and reduced degranulation of CTL, NK, and NKT cells in the liver during hepatitis (2, 3), although IFN- lambda 4 itself shows in vitro activity against hepatitis C virus (4). IFN-lambda 4 is expressed in the liver of some chronic hepatitis C patients (5). It signals through a receptor complex containing IL-28 R/IFN-lambda R1 and IL-10 R beta to induce the expression of several interferon stimulated genes (ISG) (2, 4). Alternative splicing generates additional isoforms with large internal deletions or a substituted C-terminal region.
  • References:
    1. Wack, A. et al. (2015) Nat. Immunol. 16:802.
    2. Prokunina-Olsson, L. et al. (2013) Nat. Genet. 45:164.
    3. Jouvin-Marche, E. et al. (2014) J. Infec. Dis. 209:1907.
    4. Hamming, O.J. et al. (2013) EMBO J. 32:3055.
    5. Amanzada, A. et al. (2013) PLoS One 8:e84026.
  • Long Name:
    Interferon, Lambda 4
  • Entrez Gene IDs:
    101180976 (Human)
  • Alternate Names:
    IFNAN; IFNL4; IFNlambda 4; IFN-lambda 4
Related Research Areas

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