Recombinant Human IFN-lambda 4 Protein, CF

Catalog # Availability Size / Price Qty
9165-IF-025
Recombinant Human IFN-lambda 4 Protein Bioactivity
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Citations (6)
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Recombinant Human IFN-lambda 4 Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED50 for this effect is 0.2-1.2 μg/mL.
Source
E. coli-derived human IFN-lambda 4 protein
Ala23-Leu179 with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met, Ala23
Predicted Molecular Mass
18 kDa
SDS-PAGE
19 kDa, reducing conditions

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9165-IF

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9165-IF

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human IFN-lambda 4 Protein Bioactivity View Larger

Recombinant Human IFN-lambda 4 (Catalog # 9165-IF) exhibits anti-viral activity in HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-1.2 μg/mL.

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Background: IFN-lambda 4

Interferon-lambda 4 (IFN-lambda 4) is a secreted, approximately 17 kDa member of the type III interferon family of molecules (1). It is generated by a frameshift mutation based on a TT > ∆G polymorphism (2). The TT allele inactivates expression of IFN-lambda 4 (2). The ∆G allele is associated with reduced clearance of hepatitis C virus and reduced degranulation of CTL, NK, and NKT cells in the liver during hepatitis (2, 3), although IFN- lambda 4 itself shows in vitro activity against hepatitis C virus (4). IFN-lambda 4 is expressed in the liver of some chronic hepatitis C patients (5). It signals through a receptor complex containing IL-28 R/IFN-lambda R1 and IL-10 R beta to induce the expression of several interferon stimulated genes (ISG) (2, 4). Alternative splicing generates additional isoforms with large internal deletions or a substituted C-terminal region.

References
  1. Wack, A. et al. (2015) Nat. Immunol. 16:802.
  2. Prokunina-Olsson, L. et al. (2013) Nat. Genet. 45:164.
  3. Jouvin-Marche, E. et al. (2014) J. Infec. Dis. 209:1907.
  4. Hamming, O.J. et al. (2013) EMBO J. 32:3055.
  5. Amanzada, A. et al. (2013) PLoS One 8:e84026.
Long Name
Interferon, Lambda 4
Entrez Gene IDs
101180976 (Human)
Alternate Names
IFNAN; IFNL4; IFNlambda 4; IFN-lambda 4

Citations for Recombinant Human IFN-lambda 4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Distinct molecular phenotypes involving several human diseases are induced by IFN-lambda3 and IFN-lambda4 in monocyte-derived macrophages
    Authors: M De, A Bhushan, WS Grubbe, S Roy, JL Mendoza, S Chinnaswam
    Genes and immunity, 2022;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Expression of Interferons Lambda 3 and 4 Induces Identical Response in Human Liver Cell Lines Depending Exclusively on Canonical Signaling
    Authors: M Lunova, J Kubovciak, B Smolková, M Uzhytchak, K Michalova, A Dejneka, P Strnad, O Lunov, M Jirsa
    International Journal of Molecular Sciences, 2021;22(5):.
    Species: Human
    Sample Types: Whole Cell
    Applications: Bioassay
  3. Monocytes differentiated into macrophages and dendritic cells in the presence of human IFN-&lambda3 or IFN-&lambda4 show distinct phenotypes
    Authors: M De, A Bhushan, S Chinnaswam
    J Leukoc Biol, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Interferon lambda protects the female reproductive tract against Zika virus infection
    Authors: EA Caine, SM Scheaffer, N Arora, K Zaitsev, MN Artyomov, CB Coyne, KH Moley, MS Diamond
    Nat Commun, 2019;10(1):280.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Structure-based glycoengineering of interferon lambda 4 enhances its productivity and anti-viral potency
    Authors: JH Chung, SH Hong, N Seo, TS Kim, HJ An, P Lee, EC Shin, HM Kim
    Cytokine, 2019;125(0):154833.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Biolayer Light Interferometry
  6. IFN-?4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling
    Authors: AA Obajemu, N Rao, KA Dilley, JM Vargas, F Sheikh, RP Donnelly, RS Shabman, EG Meissner, L Prokunina-, OO Onabajo
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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