Recombinant Human IFN-lambda 4 Protein, CF

R&D Systems | Catalog # 9165-IF

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human IFN-lambda 4 Protein (9165-IF)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

K9M1U5

Applications

Bioactivity
View all IFN-lambda 4 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IFN-lambda 4 protein
Ala23-Leu179 with an N-terminal Met

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met, Ala23

Predicted Molecular Mass

18 kDa

SDS-PAGE

19 kDa, reducing conditions

Activity

Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
The ED50 for this effect is 0.2-1.2 μg/mL.

Scientific Data Images for Recombinant Human IFN-lambda 4 Protein, CF

Recombinant Human IFN-lambda 4 Protein Bioactivity

Recombinant Human IFN-lambda 4 Protein Bioactivity

Recombinant Human IFN-lambda 4 (Catalog # 9165-IF) exhibits anti-viral activity in HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-1.2 μg/mL.

Formulation, Preparation, and Storage

9165-IF
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
Reconstitution

Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-lambda 4

Interferon-lambda 4 (IFN-lambda 4) is a secreted, approximately 17 kDa member of the type III interferon family of molecules (1). It is generated by a frameshift mutation based on a TT > ∆G polymorphism (2). The TT allele inactivates expression of IFN-lambda 4 (2). The ∆G allele is associated with reduced clearance of hepatitis C virus and reduced degranulation of CTL, NK, and NKT cells in the liver during hepatitis (2, 3), although IFN- lambda 4 itself shows in vitro activity against hepatitis C virus (4). IFN-lambda 4 is expressed in the liver of some chronic hepatitis C patients (5). It signals through a receptor complex containing IL-28 R/IFN-lambda R1 and IL-10 R beta to induce the expression of several interferon stimulated genes (ISG) (2, 4). Alternative splicing generates additional isoforms with large internal deletions or a substituted C-terminal region.

References

  1. Wack, A. et al. (2015) Nat. Immunol. 16:802.
  2. Prokunina-Olsson, L. et al. (2013) Nat. Genet. 45:164.
  3. Jouvin-Marche, E. et al. (2014) J. Infec. Dis. 209:1907.
  4. Hamming, O.J. et al. (2013) EMBO J. 32:3055.
  5. Amanzada, A. et al. (2013) PLoS One 8:e84026.

Long Name

Interferon, Lambda 4

Alternate Names

IFNAN, IFNL4, IFNlambda 4

Entrez Gene IDs

101180976 (Human)

Gene Symbol

IFNL4

UniProt

K9M1U5

Additional IFN-lambda 4 Products

Product Documents for Recombinant Human IFN-lambda 4 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human IFN-lambda 4 Protein, CF

For research use only

Related Research Areas

Interferons

Citations for Recombinant Human IFN-lambda 4 Protein, CF

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