Recombinant Human IL-10 R beta Fc Chimera Protein, CF

R&D Systems | Catalog # 11460-RB

CHO expressed
R&D Systems
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human IL-10 R beta Fc Chimera Protein (11460-RB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q08334.2

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all IL-10 R beta Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-10 R beta protein
Human IL-10 R beta
(Met20-Ser220)
Accession # Q08334.2		 GGIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met20

Predicted Molecular Mass

50 kDa

SDS-PAGE

72-82 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human IL-10 R beta Fc Chimera (Catalog # 11460-RB) binds Recombinant Human IL-28 R alpha /IFN-lambda R1 Fc Chimera (Catalog # 5260-MR) in the presence of Recombinant Human IL-28B/IFN-lambda 3 (Catalog # 5259-IL/CF) with an ED50 of 0.250-3.00 µg/mL.

Scientific Data Images for Recombinant Human IL-10 R beta Fc Chimera Protein, CF

Recombinant Human IL‑10 R beta Fc Chimera Protein Binding Activity.

Recombinant Human IL-10 R beta Fc Chimera Protein (Catalog # 11460-RB) binds Recombinant Human IL-28 R alpha /IFN-lambda R1 Fc Chimera (5260-MR) in the presence of Recombinant Human IL-28B/IFN-lambda 3 (5259-IL/CF) with an ED50 of 0.250-3.00 μg/mL.

Recombinant Human IL‑10 R beta Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL‑10 R beta Fc Chimera Protein (Catalog # 11460-RB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 72-82 kDa and 140-165 kDa, respectively..

Formulation, Preparation, and Storage

11460-RB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10 R beta

Interleukin-10 Receptor beta (IL-10 R beta ), also known as IL-10 R2 and CRF2-4, is a 60 kDa transmembrane glycoprotein that functions as a co-receptor for several class 2 cytokines including Interleukins-10, -22, -26, -28A/IFN-lambda 2, -28B/IFN-lambda 3, and -29/IFN-lambda (1, 2). IL-10 R beta associates with ligand‑specific receptor subunits to form signaling receptor complexes, e.g. IL-10 R alpha for IL-10 (3, 4), IL-20 R alpha for IL-26 (5, 6), IL-22 R alpha for IL-22 (7, 8), and IL-28 R alpha for IL-28A, IL-28B, and IL-29 (9, 10). IL‑10 R beta is widely expressed, while the associated receptor subunits exhibit differential expression patterns (1). The ligand‑specific subunits are responsible for the divergent functions of these cytokines, encompassing immune suppression, promotion or inhibition of inflammation, mucosal defense, antiviral immunity, and hematopoiesis (1). IL-10 R beta deficient mice lack responsiveness to each of those cytokines. IL-10 R beta contributes to ligand binding, but effective signaling is only triggered in the presence of the ligand‑specific subunit (8, 9, 11). In the case of IL-10, a cytokine dimer binds to two IL‑10 R alpha /IL-10R1 chains, resulting in recruitment of two IL-10 R beta /IL-10R2 chains (3, 12). Some members of the IL-10 family are monomeric cytokines and interact with single molecules of IL-10 R beta and their ligand‑specific subunit (1). Mature human IL-10 R beta consists of a 201 amino acid (aa) extracellular region with two fibronectin type-III domains, a 22 aa transmembrane segment and a 83 aa cytoplasmic domain (13). Within the ECD, human IL-10 R beta shares 75% and 78% aa sequence identity with mouse and rat IL-10 R beta, respectively.

References

  1. Commins, S. et al. (2008) J. Allergy Clin. Immunol. 121:1108.
  2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  3. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  4. Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
  5. Sheikh, F. et al. (2004) J. Immunol. 172:2006.
  6. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  7. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  8. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
  9. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  10. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  11. Yoon, S.I. et al. (2006) J. Biol. Chem. 281:35088.
  12. Pletnev, S. et al. (2005) BMC Struct. Biol. 5:10.
  13. Lutfalla, G. et al. (1993) Genomics 16:366.

Long Name

Interleukin 10 Receptor beta

Alternate Names

CDw210b, CRFB4, D21S58, IL-10R2, IL-10Rb, IL10R beta, IL10RB

Entrez Gene IDs

3588 (Human); 16155 (Mouse)

Gene Symbol

IL10RB

UniProt

Q08334.2

Additional IL-10 R beta Products

Product Documents for Recombinant Human IL-10 R beta Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-10 R beta Fc Chimera Protein, CF

For research use only

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Associated Pathways

Jak/STAT Signaling Pathway Jak/STAT Signaling Pathway Thumbnail