Recombinant Human IL-17A, Biotinylated Protein

Catalog #: BT7955 Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
BT7955-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
BT7955-025

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Recombinant Human IL-17A, Biotinylated Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17A, Biotinylated Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED₅₀ for this effect is 1.5-7.5 ng/mL. Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.12-1.2 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human IL-17/IL-17A protein
Gly24-Ala155

Accession #

Q16552

N-terminal Sequence
Analysis

Gly24

Structure / Form

Disulfide-linked homodimer, Biotinylated via sugars

Predicted Molecular Mass

15 kDa (unlabeled)

SDS-PAGE

14-23 kDa, reducing conditions

Product Datasheets

BT7955 (with carrier)

Product Datasheet

COA

SDS

BT7955/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT7955

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.

Reconstitution

Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage:

BT7955/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Both Biotinylated Recombinant Human IL-17A (Catalog # BT7955) and unlabeled Recombinant Human IL-17A (Catalog # 7955-IL) induces IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 1.5-7.5 ng/mL. The similarity in activity highlights that the biotinylated protein is fully functional.

Reconstitution Calculator

Background: IL-17/IL-17A

Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17A shares 60% amino acid sequence identity with mouse and rat IL-17A (3, 4). IL-17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9-12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides
(9-11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15).
IL-17A has been shown to exert either tumorigenic or anti-tumor effects (16, 17).

References

Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
Yao, Z. et al. (1995) J. Immunol. 155:5483.
Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
Wright, J.F. et al. (2008) J. Immunol. 181:2799.
Rong, Z. et al. (2009) Cell Res. 19:208.
Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
Liang, S.C. et al. (2007) J. Immunol. 179:7791.
O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
Hsu, H. et al. (2008) Nat. Immunol. 9:166.
Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
Wang, L. et al. (2009) J. Exp. Med. 206:1457.
Kryczek, I. et al. (2009) Blood 114:357.

Long Name

Interleukin 17

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A