Recombinant Human IL-26/AK155 Dimer Protein, CF 1870-IL-010/CF: R&D Systems

Recombinant Human IL-26/AK155 Dimer Protein, CF

(3 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.1 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. Hor, S. et al. (2004) J. Biol. Chem. 279:33343. The ED50 for this effect is 0.04-0.2 µg/mL.
  • Source
    E. coli-derived Lys22-Gln171, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Structure / Form

    Disulfide-linked homodimer and a small amount of monomer (no more than 10%)

  • Predicted Molecular Mass
    17.7 kDa (monomer)
  • SDS-PAGE
    18 kDa, reducing conditions
    36 kDa, non-reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1870-IL/CF
 
1870-IL
Formulation Supplied as a 0.2 μm filtered solution in NaH2PO4, NaCl and Glycerol.
Formulation Supplied as a 0.2 μm filtered solution in NaH2PO4, NaCl and Glycerol with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Background: IL-26/AK155

IL-26 was originally cloned from herpesvirus saimiri (HVS)-transformed T-cells and named AK155 (1). It is a member of the IL-10 family of class II cytokines that signal via heterodimeric receptor complexes composed of two type I transmembrane receptor subunits (2). The human IL-26 gene has been mapped to chromosome 12q15. It encodes a 171 amino acid polypeptide with a 21 amino acid signal peptide. In addition to HVS-transformed T cells, IL-26 is also expressed in other virus transformed T cell lines, fresh peripheral mononuclear cells, activated NK cells and T cells. A mouse homologue of human IL-26 has not been identified. IL-26 binds with high-affinity to the heterodimeric complex consisting of the ligand-binding IL-20 R alpha and non ligand-binding IL-10 R beta (3). Activation of the receptor complex results in rapid phosphorylation of STAT1 and STAT3. Although the IL-26 receptor complex is highly specific for IL-26 and is not activated by other class II cytokines, the individual subunits of the IL-26 receptor complex are components in receptor complexes for other class II cytokines (1). IL-20 R alpha can form dimers with IL-20 R beta to function as signaling receptors for IL-19, IL-20, and IL-24. IL-10 R beta  can complex with IL-10 R alpha, IL-22 R, and IL-28 R alpha  to transduce signals for IL-10, IL-22, and the three novel IFNs (IL-28A, IL-28B and IL-29), respectively. The physiological functions of IL-26 remain to be determined.

IL-26 was reported to be a homodimer in solution (1). E. coli-derived IL-26 produced by R&D Systems contains disulfide-linked homodimers and monomers. Both the pure monomeric and dimeric IL-26 can bind directly to IL-20 R alpha  and induce STAT activation in COLO-205 cells (4). Besides IL-20 R alpha R&D Systems IL-26 preparations have also been shown to bind IL-20 R beta. The significance of this receptor-ligand interaction has not been investigated.

  • References:
    1. Knappe. A. et al. (2000) J. Virology 74:3881.
    2. Renauld, J.-C. (2003) Nature Reviews Immunology 3:667.
    3. Sheikh, F. et al. (2004) J. Immunol. 172:2006.
    4. Unpublished data (2003) R&D Systems, Inc.
  • Long Name:
    Interleukin 26
  • Entrez Gene IDs:
    55801 (Human)
  • Alternate Names:
    AK155; AK155IL-26interleukin-26; IL26; IL-26; interleukin 26; Protein AK155
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Species
Applications
Sample Type
  1. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012;10(9):e1001395.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Cutting edge: IL-26 signals through a novel receptor complex composed of IL-20 receptor 1 and IL-10 receptor 2.
    Authors: Sheikh F, Baurin VV, Lewis-Antes A, Shah NK, Smirnov SV, Anantha S, Dickensheets H, Dumoutier L, Renauld JC, Zdanov A, Donnelly RP, Kotenko SV
    J. Immunol., 2004;172(4):2006-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Binding Assay

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