Recombinant Human IL-27 Protein

Formulations:
Catalog # Availability Size / Price Qty
2526-IL-010
Product Details
Citations (32)
FAQs
Reviews (4)

Recombinant Human IL-27 Protein Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585. The ED50 for this effect is 1-8 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-27 protein
Human IL-27 EBI-3
(Arg21-Lys229)
Accession # Q14213.2
GSGSSRGGSGSGGSGGGGSKL  Human IL-27 p28
(Phe29-Pro243)
Accession # AAM34498
6-His tag
N-terminus C-terminus
N-terminal Sequence
Analysis
Arg21
Predicted Molecular Mass
50 kDa
SDS-PAGE
60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2526-IL

Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2526-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA and CHAPS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-27

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family (5). Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region (6). The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (7). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1 (6, 8, 9). IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 7). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (10).

References
  1. Boulay, J-L. et al. (2003) Immunity 19:159. 
  2. Trinchieri, G. et al. (2003) Immunity 19:641. 
  3. Murakami, M. et al. (2004) Growth Factors 22:75. 
  4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Exp. Opin. Biol. Ther. 3:715. 
  5. Devergne, O. et al. (1996) J. Virology 70:1143. 
  6. Pflanz, S. et al. (2002) Immunity 16:779.
  7. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
  8. Pflanz, S. et al. (2004) J Immunol 172:2225.
  9. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
  10. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl).193:1.
Long Name
Interleukin 27
Entrez Gene IDs
246778 (Human); 246779 (Mouse); 102121242 (Cynomolgus Monkey)
Alternate Names
IL-27 p28 subunit; IL-27 subunit alpha; IL27; IL-27; IL27A; IL-27-A; IL27-A; IL27p28; IL30; interleukin 27; interleukin 30; interleukin-27 subunit alpha; MGC71873; p28IL-27A

Citations for Recombinant Human IL-27 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Plasma levels of interleukin 27 in falciparum malaria is increased independently of co-infection with HIV: potential immune-regulatory role during malaria
    Authors: K Otterdal, A Berg, AE Michelsen, S Patel, I Gregersen, EL Sagen, B Halvorsen, A Yndestad, T Ueland, N Langeland, P Aukrust
    BMC Infect. Dis., 2020;20(1):65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Profiles of Long Non-Coding RNAs and mRNA Expression in Human Macrophages Regulated by Interleukin-27
    Authors: X Hu, S Goswami, J Qiu, Q Chen, S Laverdure, BT Sherman, T Imamichi
    Int J Mol Sci, 2019;20(24):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. N4BP1 restricts HIV-1 and its inactivation by MALT1 promotes viral reactivation
    Authors: D Yamasoba, K Sato, T Ichinose, T Imamura, L Koepke, S Joas, E Reith, D Hotter, N Misawa, K Akaki, T Uehata, T Mino, S Miyamoto, T Noda, A Yamashita, DM Standley, F Kirchhoff, D Sauter, Y Koyanagi, O Takeuchi
    Nat Microbiol, 2019;0(0):.
    Species: Human
    Sample Types: Primary Cells
    Applications: Bioassay
  4. The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies
    Authors: C Rolvering, AD Zimmer, A Ginolhac, C Margue, M Kirchmeyer, F Servais, HM Hermanns, S Hergovits, PV Nazarov, N Nicot, S Kreis, S Haan, I Behrmann, C Haan
    J. Leukoc. Biol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. IL-27 Expression and Responsiveness in Human Uterine Epithelial Cells and Fibroblasts In Vitro and the Role of Estradiol
    Authors: MV Patel, Z Shen, RM Rossoll, CR Wira
    J. Interferon Cytokine Res., 2018;38(3):101-110.
    Species: Human
    Sample Types: cell culture
    Applications: Bioassay
  6. The effects of CD14 and IL-27 on induction of endotoxin tolerance in human monocytes and macrophages
    Authors: C Petes, V Mintsopoul, RL Finnen, BW Banfield, K Gee
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
  7. IL-27 amplifies cytokine responses to Gram-negative bacterial products and Salmonella typhimurium infection
    Authors: C Petes, N Odoardi, SM Plater, NL Martin, K Gee
    Sci Rep, 2018;8(1):13704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. IL-27 regulates HIF-1?-mediated VEGFA response in macrophages of diabetic retinopathy patients and healthy individuals
    Authors: Q Zhang, APD Cunha, S Li, Q Hao, V Kainz, Q Huang, HY Wu
    Cytokine, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. BST-2 Expression Modulates Small CD4 Mimetic Sensitization of HIV-1-infected cells to ADCC
    Authors: J Richard, J Prévost, B von Bredow, S Ding, N Brassard, H Medjahed, M Coutu, B Melillo, F Bibollet-R, BH Hahn, DE Kaufmann, AB Smith, J Sodroski, D Sauter, F Kirchhoff, K Gee, SJ Neil, DT Evans, A Finzi
    J. Virol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. PD-L1 up-regulation restrains Th17 cell differentiation in STAT3 loss- and STAT1 gain-of-function patients.
    Authors: Yuan Zhang, Chi A Ma, Monica G Lawrence, Timothy J Break, Michael P O'connell, Jonathan J Lyons, Diego B López, John S Barber, Yongge Zhao, Daniel L Barber, Alexandra F Freeman, Steven M Holland, Michail S Lionakis, Joshua D Milner
    The Journal of Experimental Medicine, 2017;0(0):1540-9538.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-?
    Authors: KO Dixon, J O'Flynn, N Klar-Moham, MR Daha, C van Kooten
    Eur. J. Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation
    Authors: I Gregersen, Ø Sandanger, ET Askevold, EL Sagen, K Yang, S Holm, TM Pedersen, M Skjelland, K Krohg-Søre, TV Hansen, TB Dahl, K Otterdal, T Espevik, P Aukrust, A Yndestad, B Halvorsen
    PLoS ONE, 2017;12(11):e0188387.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Crosstalk between different family members: IL27 recapitulates IFN? responses in HCC cells, but is inhibited by IL6-type cytokines
    Authors: Catherine Rolvering
    Biochim. Biophys. Acta, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. IL-27 Suppresses Antimicrobial Activity in Human Leprosy.
    Authors: Teles R, Kelly-Scumpia K, Sarno E, Rea T, Ochoa M, Cheng G, Modlin R
    J Invest Dermatol, 2015;135(10):2410-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. EBI3 Downregulation Contributes to Type I Collagen Overexpression in Scleroderma Skin.
    Authors: Kudo H, Wang Z, Jinnin M, Nakayama W, Inoue K, Honda N, Nakashima T, Kajihara I, Makino K, Makino T, Fukushima S, Ihn H
    J Immunol, 2015;195(8):3565-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway.
    Authors: Sha X, Meng S, Li X, Xi H, Maddaloni M, Pascual D, Shan H, Jiang X, Wang H, Yang X
    J Biol Chem, 2015;290(31):19307-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014;192(6):2634-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. IL-27 driven upregulation of surface HLA-E expression on monocytes inhibits IFN-gamma release by autologous NK cells.
    Authors: Morandi F, Airoldi I, Pistoia V
    J Immunol Res, 2014;2014(0):938561.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. IL-27, a cytokine, and IFN-lambda1, a type III IFN, are coordinated to regulate virus replication through type I IFN.
    Authors: Cao Y, Zhang R, Zhang W, Zhu C, Yu Y, Song Y, Wang Q, Bai L, Liu Y, Wu K, Wu J
    J Immunol, 2014;192(2):691-703.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of human tegumentary leishmaniasis.
    Authors: Oliveira W, Ribeiro L, Schrieffer A, Machado P, Carvalho E, Bacellar O
    Cytokine, 2013;66(2):127-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway.
    Authors: Chen, Qian, Swaminathan, Sanjay, Yang, De, Dai, Lue, Sui, Hongyan, Yang, Jun, Hornung, Ronald L, Wang, Yanmei, Huang, Da Wei, Hu, Xiaojun, Lempicki, Richard, Imamichi, Tomozumi
    PLoS ONE, 2013;8(3):e59194.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Independent and interdependent immunoregulatory effects of IL-27, IFN-beta, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis.
    Authors: Fitzgerald D, Fonseca-Kelly Z, Cullimore M, Safabakhsh P, Saris C, Zhang G, Rostami A
    J Immunol, 2013;190(7):3225-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. IL-27 inhibits epithelial-mesenchymal transition and angiogenic factor production in a STAT1-dominant pathway in human non-small cell lung cancer.
    Authors: Kachroo P, Lee M, Zhang L, Baratelli F, Lee G, Srivastava M, Wang G, Walser T, Krysan K, Sharma S, Dubinett S, Lee J
    J Exp Clin Cancer Res, 2013;32(0):97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  24. Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid.
    Authors: Tzeng, Tsai-Ten, Tsay, Huey-Jen, Chang, Luping, Hsu, Chia-Lin, Lai, Tzu-Hsua, Huang, Fong-Lee, Shiao, Young-Ji
    J Biomed Sci, 2013;20(0):90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo.
    Authors: Cocco C, Di Carlo E, Zupo S
    Leukemia, 2012;26(6):1365-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Persistent loss of IL-27 responsiveness in CD8+ memory T cells abrogates IL-10 expression in a recall response.
    Authors: Perona-Wright G, Kohlmeier J, Bassity E, Freitas T, Mohrs K, Cookenham T, Situ H, Pearce E, Woodland D, Mohrs M
    Proc Natl Acad Sci U S A, 2012;109(45):18535-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Human TCR gamma delta+ T cells represent a novel target for IL-27 activity.
    Authors: Morandi F, Prigione I, Airoldi I
    Eur. J. Immunol., 2012;42(6):1547-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Influenza A virus induces interleukin-27 through cyclooxygenase-2 and protein kinase A signaling.
    Authors: Liu L, Cao Z, Chen J
    J. Biol. Chem., 2012;287(15):11899-910.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. IL-27-Induced Gene Expression Is Downregulated in HIV-Infected Subjects.
    Authors: Guzzo C, Hopman W, Che Mat N, Wobeser W, Gee K
    PLoS ONE, 2012;7(9):e45706.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Interleukin-27 acts as multifunctional antitumor agent in multiple myeloma.
    Authors: Cocco C, Giuliani N, Di Carlo E
    Clin. Cancer Res., 2010;16(16):4188-97.
    Species: Complex Species Category
    Sample Types: In Vivo
    Applications: In Vivo
  32. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
    Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
    J. Immunol., 2010;185(11):7047-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human IL-27 Protein

Average Rating: 4.5 (Based on 4 Reviews)

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Recombinant Human IL-27 Protein
By Anonymous on 06/16/2020
Application: Stem/Immune cell maintenance or differentiation

Recombinant Human IL-27 Protein
By Anonymous on 12/02/2019
Application: Immunoassay Standard

Recombinant Human IL-27 Protein
By Anonymous on 03/28/2018
Application: Stem/Immune cell maintenance or differentiation

Recombinant Human IL-27 Protein
By Anonymous on 11/27/2017
Application: Immunoassay Standard

AF2526 was used as the solid phase antibody, BAF2526 was used as the detection antibody. 2526-IL was used as the immunoassay standard. Sensitivity of the assay was around 100 pg/ml.