Recombinant Human IL-27 Ra/WSX-1/TCCR Fc Chimera Protein, CF

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1479-TC-050
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Recombinant Human IL-27 Ra/WSX-1/TCCR Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-27 anti-viral activity using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585. The ED50 for this effect is 0.75‑4.5 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-27 R alpha/WSX-1/TCCR protein
Human IL-27 R alpha
(Gly34-Lys516)
Accession # Q6UWB1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Gly34
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
79 kDa (monomer)
SDS-PAGE
100-110 kDa, reducing conditions

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1479-TC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1479-TC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IL-27 R alpha/WSX-1/TCCR

IL‑27 R alpha (also known as WSX‑1 and TCCR) is a 96 ‑ 100 kDa member of the type I, group 2 cytokine receptor family (1, 2, 3, 4, 5, 6). Mature IL‑27 R alpha  is a type I transmembrane glycoprotein that contains a 484 amino acid (aa) extracellular region, a 21 aa transmembrane segment and a 99 aa cytoplasmic domain. Consistent with type I cytokine receptors, the extracellular region contains four positionally conserved cysteine residues, a WSxWS motif (for receptor folding and ligand binding), and three fibronectin type III repeats. The intracellular domain contains a "box‑1" motif that may be involved with Janus kinases (3). One potential alternate splice form has been hypothesized that involves a 58 aa addition to the cytoplasmic domain and, based on mouse, a soluble 33 kDa splice form that shows a 20 aa substitution for aa 257 ‑ 636 may also occur in human (3, 7). The human IL‑27 R alpha extracellular region shares 63% amino acid identity with the mouse IL‑27 R alpha extracellular domain (2, 3). IL‑27 R alpha is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and naïve T cells (1, 2, 4, 8). Typical of other class I cytokine receptor chains, the ligand binding IL‑27 R alpha molecule is known to heterodimerize with a signal‑transducing subunit (gp130) to form a functional IL‑27 receptor (9, 10). In addition, IL‑27 R alpha is reported to complex with CNTFR alpha and gp130 form a humanin receptor on neurons (7, 11), and to complex with gp130 and IL‑6 R to form a receptor for a p28:CLF heterodimeric cytokine on lymphocytes (12). Studies using IL‑27 R alpha /WSX‑1‑/‑ mice reveal that IL‑27 has the ability to suppress T cell activity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity (4, 13, 14). In particular, IL‑27 is known to act on naïve T cells, blocking their differentiation into a Th17 phenotype. Notably, cells committed to a Th17 phenotype, although they express a functional IL‑27 receptor, are unresponsive to the effects of IL‑27 (15). Activated T cells that are CD4+ and CD8+, and which express the IL‑27 receptor, can be induced by
IL‑27 to form a double‑positive CD25+ FoxP3 IFN‑ gamma plus IL‑10 secreting phenotype that both promotes and suppresses the inflammatory response (16).

References
  1. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
  2. Chen, Q. et al. (2000) Nature 407:916.
  3. Sprecher, C.A. et al. (1998) Biochem. Biophys. Res. Commun. 246:82.
  4. Artis, D. et al. (2004) J. Immunol. 173:5626.
  5. Yoshida, H. & Y. Miyazaki (2008) Int. J. Biochem. Cell Biol. 40:2379.
  6. Yoshida, H. & M. Yoshiyuki (2008) Immunol. Rev. 226:234.
  7. Hashimoto, Y. et al. (2009) Biochem. Biophys. Res. Commun. 389:95.
  8. Holscher, C. et al. (2005) J. Immunol. 174:3534.
  9. Pflanz, S. et al. (2004) J. Immunol. 172:2225.
  10. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
  11. Hashimoto, Y. et al. (2009) Mol. Biol. Cell 20:2864.
  12. Crabe, S. et al. (2009) J. Immunol. 183:7692.
  13. Villarino, A. et al. (2003) J. Immunol. 170:645.
  14. Hamano., S. et al. (2003) Immunity 19:657.
  15. El-behi, M. et al. (2009) J. Immunol. 183:4957.
  16. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
Long Name
Interleukin-27 Receptor Subunit alpha
Entrez Gene IDs
9466 (Human); 50931 (Mouse)
Alternate Names
class I cytokine receptor; CRL1IL-27R; Cytokine receptor-like 1; IL-27 R alpha; IL27R alpha; IL27R; IL27RA; IL-27Ra; IL-27R-alpha; interleukin 27 receptor, alpha; interleukin-27 receptor subunit alpha; TCCR; TCCRIL-27 receptor subunit alpha; T-cell cytokine receptor type 1; Type I T-cell cytokine receptor; WSX-1; WSX1IL-27R subunit alpha; zcytor1

Citations for Recombinant Human IL-27 Ra/WSX-1/TCCR Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
    Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
    Sci Rep, 2018;8(1):10328.
    Applications: ELISA (Standard)
  2. A soluble form of IL-27Ralpha is a natural IL-27 antagonist.
    Authors: Dietrich C, Candon S, Ruemmele F, Devergne O
    J Immunol, 2014;192(11):5382-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Standard)

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