Recombinant Human IL-28B/IFN-lambda 3 Protein

Formulations:
Catalog # Availability Size / Price Qty
5259-IL-025
Product Details
Citations (13)
FAQs
Reviews (1)

Recombinant Human IL-28B/IFN-lambda 3 Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED50 for this effect is 1-5 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human IL-28B/IFN-lambda 3 protein
Arg30-Val200, with the variant of Lys74Arg and a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Arg30
Predicted Molecular Mass
20.1 kDa
SDS-PAGE
22 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5259-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5259-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-28B/IFN-lambda 3

IL-28B (also named interferon-lambda 3, IFN-lambda 3), IL-28A (IFN-lambda 2) and IL-29 (IFN-lambda 1) are type III interferons that are class II cytokine receptor ligands (1-4). They are distantly related to members of the IL-10 family and type I IFN family (1- 4). Human IL-28B cDNA encodes a 200 amino acid (aa) protein with a 25 aa signal peptide and a 175 aa mature protein that lacks N-glycosylation sites. Mature human IL-28B shares 64% and 75% aa sequence identity with mouse and canine IL-28B, respectively, and is active across species (5). Human IL-28B shares 94% and 69% aa identity with human IL-28A and IL‑29, respectively (4). Type III interferons are widely expressed, but are mainly produced by antigen presenting cells in response to viruses and double-stranded RNA that interact with Toll-like receptors or RIG-1 family helicases (2-6). They signal through a widely expressed receptor that is a heterodimer of the IL-10 receptor beta (IL-10 R beta ) and IL-28 receptor alpha (IL-28 R alpha ; also called IFN-lambda R1) (2, 3, 7, 9). Interaction of either type I or type III IFNs with their receptors activates similar pathways, including JAK tyrosine kinase activation, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1-3). Both type I and III IFNs induce anti‑viral activity and up‑regulate MHC class I antigen expression (2-6). Cell lines responsive to type III IFNs are also responsive to type I IFNs, but in general, higher concentrations of type III IFNs are needed for similar in vitro responses (8). In vivo, however, type III IFNs enhance levels of IFN-gamma in serum, suggesting that the robust anti-viral activity of type III IFNs may stem in part from activation of the immune system (5, 7). Anti-proliferative and antitumor activity in vivo has also been shown for type III IFNs (9-11).

References
  1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
  2. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  4. Bartlett, N.W. et al. (2005) J. Gen. Virol. 86:1589.
  5. Ank, N. et al. (2006) J. Virol. 80:4501.
  6. Onoguchi, K. et al. (2007) J. Biol. Chem. 282:7576.
  7. Siebler, J. et al. (2007) Gastroenterology 132:358.
  8. Meager, A. et al. (2005) Cytokine 31:109.
  9. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
  10. Sato, A. et al. (2006) J. Immunol. 176:7686.
  11. Zitzmann, K. et al. (2006) Biochem. Biophys. Res. Commun. 344:1334.
Long Name
Interleukin 28B
Entrez Gene IDs
282617 (Human); 338374 (Mouse)
Alternate Names
interleukin-28B; IFN-lambda 3; IL28B; IL-28B; IL28C; interferon, lambda 3

Citations for Recombinant Human IL-28B/IFN-lambda 3 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Structure-based glycoengineering of interferon lambda 4 enhances its productivity and anti-viral potency
    Authors: JH Chung, SH Hong, N Seo, TS Kim, HJ An, P Lee, EC Shin, HM Kim
    Cytokine, 2019;125(0):154833.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Type III interferon signaling restricts enterovirus 71 infection of goblet cells
    Authors: C Good, AI Wells, CB Coyne
    Sci Adv, 2019;5(3):eaau4255.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Adenovirus infection of human enteroids reveals interferon sensitivity and preferential infection of goblet cells
    Authors: MK Holly, JG Smith
    J. Virol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. IFN-?4 potently blocks IFN-? signalling by ISG15 and USP18 in hepatitis C virus infection
    Authors: PS Sung, SH Hong, JH Chung, S Kim, SH Park, HM Kim, SK Yoon, EC Shin
    Sci Rep, 2017;7(1):3821.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Resistin reinforces interferon ?-3 to eliminate hepatitis C virus with fine-tuning from RETN single-nucleotide polymorphisms
    Sci Rep, 2016;6(0):30799.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Hepatitis-C-virus-induced microRNAs dampen interferon-mediated antiviral signaling
    Nat. Med., 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Immune Cell Profiling of IFN-? Response Shows pDCs Express Highest Level of IFN-?R1 and Are Directly Responsive Via the JAK-STAT Pathway
    J Interferon Cytokine Res, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Impact and regulation of lambda interferon response in human metapneumovirus infection.
    Authors: Banos-Lara M, Harvey L, Mendoza A, Simms D, Chouljenko V, Wakamatsu N, Kousoulas K, Guerrero-Plata A
    J Virol, 2015;89(1):730-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Epigenetic reprogramming of the type III interferon response potentiates antiviral activity and suppresses tumor growth.
    Authors: Ding S, Khoury-Hanold W, Iwasaki A, Robek M
    PLoS Biol, 2014;12(1):e1001758.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Respiratory syncytial virus can infect basal cells and alter human airway epithelial differentiation.
    Authors: Persson B, Jaffe A, Fearns R, Danahay H
    PLoS ONE, 2014;9(7):e102368.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells.
    Authors: Stone, Amy E L, Giugliano, Silvia, Schnell, Gretja, Cheng, Linling, Leahy, Katelyn, Golden-Mason, Lucy, Gale, Michael, Rosen, Hugo R
    PLoS Pathog, 2013;9(4):e1003316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. IFN-lambda3 inhibits HIV infection of macrophages through the JAK-STAT pathway.
    Authors: Liu MQ, Zhou DJ, Wang X, Zhou W, Ye L, Li JL, Wang YZ, Ho WZ
    PLoS ONE, 2012;7(4):e35902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human IL-28B/IFN-lambda 3 Protein
By Anonymous on 12/01/2017
Application: In vitro bioactivity in cell culture