Recombinant Human IL-32 gamma Protein, CF

Catalog # Availability Size / Price Qty
4690-IL-025/CF
Product Details
Citations (1)
FAQs
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Recombinant Human IL-32 gamma Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells under serum free conditions in the presence of 10 µg/mL of Polymyxin B. Kim, S.H. et al. (2005) Immunity 22:131. The ED50 for this effect is 30-180 ng/mL.
Source
E. coli-derived human IL-32 gamma protein
MNHKVHHHHHH Human IL-32 gamma
(Met1 - Lys234)
Accession # P24001
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
28.1 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4690-IL/CF

Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and CHAPS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after opening.
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Background: IL-32 gamma

Interleukin-32 gamma (IL-32 gamma ), a proinflammatory cytokine previously termed natural killer cells transcript 4 (NK4) (also tumor necrosis factor alpha (TNF alpha ) -inducing factor), is a 27 kDa, secreted glycoprotein that belongs to no known cytokine family (1). Little is known about it. However, because it has the ability to induce proinflammatory cytokines such as TNF alpha and IL-8 in THP-1 cells, and activates typical cytokine signaling pathways involving NF-kappa B and p38, IL-32 is considered to be a cytokine (2). Human IL-32 gamma is 234 amino acids (aa) in length (1). It contains a 30 aa signal sequence, plus a 204 aa mature region that possesses three potential myristoylation sites and one potential N-linked glycosylation site (1, 2). IL-32 gamma represents the standard protein, and is the first isoform. In addition, there are five potential alternate splice forms (3). IL-32 alpha lacks two of the splicing variant regions found in IL-32 gamma (aa 19 - 64 and aa 154 - 210). IL-32 beta is missing aa 19 - 64, and IL-32δ is missing aa 1 - 10 and aa 19 - 64. IL-32 epsilon and IL-32 zeta are novel isoforms that have not yet been characterized. Human IL-32 gamma is active in mouse cells (2). No rodent orthologs have been reported. No putative IL-32 receptor has been found, but using the isoform IL-32 alpha, the protein proved to bind to proteinase 3 (PR3) (4, 5). IL-32 is involved in activation-induced cell death in T cells (3) and is highly expressed in tissues in a number of disease states including the synovial tissue in rheumatoid arthritis (5, 6) and human colon epithelial tissue in Crohn’s disease (5). Using siRNA to endogenous IL-32 in primary human blood monocytes, there were significant decreases (greater than 50%) in IFN-gamma, TNF, and IL-6, suggesting that endogenous IL-32 is upstream in the cytokine cascade in monocytes (7). Increased levels of IL-32 during human immunodeficiency virus (HIV) infection may play a protective role by suppressing HIV replication (8). In addition, mycobacteria species are potent stimuli for the production of IL-32 from human monocytes and macrophages (9).

References
  1. Dahl, C. et al. (1992) J. Immunol. 148:597.
  2. Kim, S-H. et al. (2005) Immunity 22:131.
  3. Goda, C. et al. (2006) Int. Immunol. 18:233.
  4. Novick, D. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3316.
  5. Dinarello C. and S-H. Kim (2006) Ann. Rheum. Dis. 65(Suppl III):iii61.
  6. Joosten, L. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3298.
  7. Nold M. F. et al. (2008) J. Immuno. 180: 557.
  8. Rasool, S. T. et al. (2008) Immunol Lett. 117:161.
  9. Netea, M. et al. (2006) PloS Med. 3:1310.
Long Name
Interleukin 32 gamma
Entrez Gene IDs
9235 (Human)
Alternate Names
IL32 gamma; IL-32 gamma; IL32; IL-32alpha; IL-32beta; IL-32delta; IL-32gamma; interleukin 32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd

Citation for Recombinant Human IL-32 gamma Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-32 promotes angiogenesis.
    Authors: Nold-Petry C, Rudloff I, Baumer Y, Ruvo M, Marasco D, Botti P, Farkas L, Cho S, Zepp J, Azam T, Dinkel H, Palmer B, Boisvert W, Cool C, Taraseviciene-Stewart L, Heinhuis B, Joosten L, Dinarello C, Voelkel N, Nold M
    J Immunol, 2014;192(2):589-602.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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