Recombinant Human IL-35 Fc Chimera Protein, CF

    
Datasheet / CoA / SDS
Product Details
FAQs
Supplemental Products
Reviews
  • Purity
    >80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 R beta 2 Fc Chimera (Catalog # 1959-B2) is immobilized at 5 μg/mL (100 μL/well), the concentration of Recombinant Human IL-35 Fc Chimera that produces 50% optimal binding response is approximately 20-120 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived
    Human IL-27 beta /EBI3
    (Arg21-Lys229)
    Accession # Q14213
    (GGGS)4 Human IL-12 alpha /p35
    Arg23-Ser219
    Accession # P29459
    IEGRMD Human IgG1
    Pro100-Lys330)
  • Accession #
  • N-terminal Sequence
    Analysis
    Arg21
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    73 kDa
  • SDS-PAGE
    81-97 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

8608-IL
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human IL-12 R beta 2 Fc Chimera (Catalog # 1959-B2) isimmobilized at 5 μg/mL (100 μL/well), Recombinant Human IL-35 Fc Chimera(Catalog # 8608-IL) binds with an ED50 of
20-120 ng/mL. Recombinant Human IL-35from the two competitorshave much weaker Recombinant Human IL-12 R beta 2 Fc Chimera binding activity.
1 μg/lane of Recombinant Human IL-35 Fc Chimera Protein from R&D Systems andtwo competitors was resolved with SDS-PAGE under reducing (R) and non-reducing(NR) conditions and visualized by silver staining. Recombinant Human IL-35 Fc Chimera Protein(Catalog # 8608-IL) from R&D Systems shows single bands at 85 kDa and 185kDa, respectively. The R&D Systems Protein offers a better purity than thecompetition.
Background: IL-35

Interleukin 35 (IL-35) is a member of the IL-12 family of heterodimeric cytokines. Unlike other IL-12 family cytokines which stimulate the immune response, the predominant function of IL-35 is as an immunosuppressant. IL-12 cytokines are composed of an alpha and beta subunit which, for IL-35 are the IL-12 p35 subunit and the EBI3 subunit, respectively (1-3). The IL-12 p35 subunit of IL-35 is synthesized as a 219 amino acid (aa) precursor protein with a 22 aa signal sequence and a 197 aa mature region. The EBI3 subunit of IL-35 is synthesized as a 229 aa precursor protein that contains a 20 aa signal sequence and a 209 aa mature region. Human and mouse IL-35 share 58% and 62% sequence homology in their IL-12 p35 and EBI3 subunits, respectively. IL-35 binds to the homodimeric receptors, IL-12 R beta 2 and gp130, as well as to the IL-12 R beta 2-gp130 receptor heterodimer (4). The expression pattern of IL-35 is thought to differ between mouse and humans (5). In mouse regulatory T cells, both subunits of IL-35 are constitutively expressed and the mature IL-35 is secreted. In humans, IL-12 p35 is the only subunit constitutively expressed in regulatory T cells. Immune activation can induce EBI3 expression and IL-35 secretion in human effector T cells (6-8). IL-35 is also expressed and secreted in human placental trophoblasts (1, 9). In both human and mouse IL-35 has been shown to suppress effector T cell proliferation, inhibit Th17 cell development, and promote the conversion of T cells and B cells into regulatory T and B cells, respectively (1, 4, 8, 10, 11). IL-35 is thought to be involved in infectious tolerance and inflammatory cytokine-mediated autoimmune disorders (1, 3, 5, 12). Serum levels of IL-35 are associated with acute graft-versus-host disease following hematopoietic stem cell transplantation (13, 14). IL-35 also functions as a regulator of tumor growth (2, 12, 15).

  • References:
    1. Collison, L.W. and D.A. Vignali (2008) Immunol. Rev. 226:248.
    2. Wang, Z. et al. (2013) J. Immunol. 190:2415.
    3. Choi, J. et al. (2015) Clin. Rev. Allergy. Immunol.
    4. Collison, L.W. et al. (2012) Nat. Immunol. 13:290.
    5. Ning-Wei, Z. (2010) Rev. Med. Chil. 138:758.
    6. Bardel, E. et al. (2008) J. Immunol. 181:6898.
    7. Guttek, K. and D. Reinhold (2013) Cytokine 64:46.
    8. Collison, L.W. et al. (2007) Nature 450:566.
    9. Mao, H. et al. (2013) Hum. Immunol. 74:872.
    10. Wang, R.X. et al. (2014) Nat. Med. 20:633.
    11. Niedbala, W. et al. (2007) Eur. J. Immunol. 37:3021.
    12. Collison, L.W. et al. (2010) Nat. Immunol. 11:1093.
    13. Liu, Y. et al. (2014) Leukemia. [Epub ahead of print; PMID: 25363669].
    14. Zhang, X. et al. (2014) Ann. Hematol. [Epub ahead of print; PMID: 25512184].
    15. Long, J. et al. (2013) Biochem. Biophys. Res. Commun. 430:364.
  • Long Name:
    Interleukin 35
  • Entrez Gene IDs:
    10148 (Human); 50498 (Mouse); 680609 (Rat)
  • Alternate Names:
    IL35; IL-35
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Recombinant Proteins
Description Application Cat# Citations Images  

Recombinant Human IgG1 Fc, CF

BA 110-HG 66
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Human IL-35 Fc Chimera Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
You May Also Like
Related Products
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee