Recombinant Human IL-35 Fc Chimera Protein, CF

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Recombinant Human IL-35 Fc Chimera Protein Bioactivity
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Product Details
Citations (2)
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Recombinant Human IL-35 Fc Chimera Protein, CF Summary

Product Specifications

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.30 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 R beta 2 Fc Chimera (Catalog # 1959-B2) is immobilized at 5 μg/mL (100 μL/well), the concentration of Recombinant Human IL-35 Fc Chimera that produces 50% optimal binding response is approximately 20-120 ng/mL.
Human embryonic kidney cell, HEK293-derived human IL-35 protein
Human IL-27 beta /EBI3
Accession # Q14213
(GGGS)4 Human IL-12 alpha /p35
Accession # P29459
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
73 kDa
81-97 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human IL-35 Fc Chimera Protein Bioactivity View Larger

When Recombinant Human IL-12 R beta 2 Fc Chimera (Catalog # 1959-B2) is immobilized at 5 μg/mL (100 μL/well), Recombinant Human IL-35 Fc Chimera (Catalog # 8608-IL) binds with an ED50 of 20-120 ng/mL. Recombinant Human IL-35 from the two competitors have much weaker Recombinant Human IL-12 R beta 2 Fc Chimera binding activity.

SDS-PAGE Recombinant Human IL-35 Fc Chimera Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human IL-35 Fc Chimera Protein from R&D Systems and two competitors was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining. Recombinant Human IL-35 Fc Chimera Protein (Catalog # 8608-IL) from R&D Systems shows single bands at 85 kDa and 185 kDa, respectively. The R&D Systems Protein offers a better purity than the competition.

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Background: IL-35

Interleukin 35 (IL-35) is a member of the IL-12 family of heterodimeric cytokines. Unlike other IL-12 family cytokines which stimulate the immune response, the predominant function of IL-35 is as an immunosuppressant. IL-12 cytokines are composed of an alpha and beta subunit which, for IL-35 are the IL-12 p35 subunit and the EBI3 subunit, respectively (1-3). The IL-12 p35 subunit of IL-35 is synthesized as a 219 amino acid (aa) precursor protein with a 22 aa signal sequence and a 197 aa mature region. The EBI3 subunit of IL-35 is synthesized as a 229 aa precursor protein that contains a 20 aa signal sequence and a 209 aa mature region. Human and mouse IL-35 share 58% and 62% sequence homology in their IL-12 p35 and EBI3 subunits, respectively. IL-35 binds to the homodimeric receptors, IL-12 R beta 2 and gp130, as well as to the IL-12 R beta 2-gp130 receptor heterodimer (4). The expression pattern of IL-35 is thought to differ between mouse and humans (5). In mouse regulatory T cells, both subunits of IL-35 are constitutively expressed and the mature IL-35 is secreted. In humans, IL-12 p35 is the only subunit constitutively expressed in regulatory T cells. Immune activation can induce EBI3 expression and IL-35 secretion in human effector T cells (6-8). IL-35 is also expressed and secreted in human placental trophoblasts (1, 9). In both human and mouse IL-35 has been shown to suppress effector T cell proliferation, inhibit Th17 cell development, and promote the conversion of T cells and B cells into regulatory T and B cells, respectively (1, 4, 8, 10, 11). IL-35 is thought to be involved in infectious tolerance and inflammatory cytokine-mediated autoimmune disorders (1, 3, 5, 12). Serum levels of IL-35 are associated with acute graft-versus-host disease following hematopoietic stem cell transplantation (13, 14). IL-35 also functions as a regulator of tumor growth (2, 12, 15).

  1. Collison, L.W. and D.A. Vignali (2008) Immunol. Rev. 226:248.
  2. Wang, Z. et al. (2013) J. Immunol. 190:2415.
  3. Choi, J. et al. (2015) Clin. Rev. Allergy. Immunol.
  4. Collison, L.W. et al. (2012) Nat. Immunol. 13:290.
  5. Ning-Wei, Z. (2010) Rev. Med. Chil. 138:758.
  6. Bardel, E. et al. (2008) J. Immunol. 181:6898.
  7. Guttek, K. and D. Reinhold (2013) Cytokine 64:46.
  8. Collison, L.W. et al. (2007) Nature 450:566.
  9. Mao, H. et al. (2013) Hum. Immunol. 74:872.
  10. Wang, R.X. et al. (2014) Nat. Med. 20:633.
  11. Niedbala, W. et al. (2007) Eur. J. Immunol. 37:3021.
  12. Collison, L.W. et al. (2010) Nat. Immunol. 11:1093.
  13. Liu, Y. et al. (2014) Leukemia. [Epub ahead of print; PMID: 25363669].
  14. Zhang, X. et al. (2014) Ann. Hematol. [Epub ahead of print; PMID: 25512184].
  15. Long, J. et al. (2013) Biochem. Biophys. Res. Commun. 430:364.
Long Name
Interleukin 35
Entrez Gene IDs
10148 (Human); 50498 (Mouse); 680609 (Rat)
Alternate Names
IL35; IL-35

Citations for Recombinant Human IL-35 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. At Embryo Implantation Site IL-35 Secreted by Trophoblast, Polarizing T Cells towards IL-35+ IL-10+ IL-4+ Th2-Type Cells, Could Favour Fetal Allograft Tolerance and Pregnancy Success
    Authors: L Lombardell, F Logiodice, O Kullolli, H Haller, C Agostinis, R Bulla, D Rukavina, MP Piccinni
    International Journal of Molecular Sciences, 2022;23(9):.
  2. IL-35 is critical in suppressing superantigenic Staphylococcus aureus-driven inflammatory Th17 responses in human nasopharynx-associated lymphoid tissue
    Authors: R Xu, RK Shears, R Sharma, M Krishna, C Webb, R Ali, X Wei, A Kadioglu, Q Zhang
    Mucosal Immunol, 2020;13(3):460-470.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture


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