Recombinant Human Integrin alpha V beta 6 Fc Protein, CF

R&D Systems | Catalog # 11649-AV

Fc Chimera
R&D Systems
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human Integrin alpha V beta 6 Fc Protein (11649-AV)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Structure / Form

Disulfide linked heterodimer

Applications

Bioactivity
View all Integrin alpha V beta 6 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Integrin alpha V beta 6 protein
Human ITGAV
(Phe31-Val992)
Accession # AAA36808.1		 IEGR Human IgG1
(Glu99-Lys330)
(with modifications)
Human ITGB6
(Gly22-Asn707)
Accession # P18564.2		 HHPIEGR Human IgG1
(Glu99-Lys330)
(with modifications)
N-terminus C-terminus

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe31 (Integrin alpha V) & Gly 22 (Integrin beta 6)

Predicted Molecular Mass

133 kDa (Integrin alpha V) & 101 kDa (Integrin beta 6)

SDS-PAGE

>190 kDa, under non-reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Integrin alpha V beta 6 Fc Chimera (Catalog # 11649-AV) binds to Recombinant Human LAP (TGF-beta 1) (Catalog # 246-LP) with an ED50 of 9.00-96.0 ng/mL.

Scientific Data Images for Recombinant Human Integrin alpha V beta 6 Fc Protein, CF

Recombinant Human Integrin alpha V beta 6 Fc Chimera Protein Binding Activity.

In a functional ELISA, Recombinant Human Integrin alpha V beta 6 Fc Chimera Protein (Catalog # 11649-AV) binds to Recombinant Human LAP (TGF-beta 1) (246-LP) with an ED50 of 9.00‑96.0 ng/mL.

Recombinant Human Integrin alpha V beta 6 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human Integrin alpha V beta 6 Fc Chimera Protein (Catalog # 11649-AV) was resolved with SDS-PAGE under non-reducing (NR) condition and visualized by Coomassie® Blue staining, showing bands at >190 kDa.

Formulation, Preparation, and Storage

11649-AV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha V beta 6

Integrin alpha V beta 6 is one of five alpha V integrins and the sole beta 6 integrin (1, 2). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 95 kDa beta 6 integrin subunits is expressed exclusively on subsets of epithelial cells, especially during development, after injury or inflammation, or on many carcinomas (2-5). The ligand interaction site of alpha V beta 6 is in the N-terminal head region formed by an interaction of the beta 6 vWFA domain with the alpha V beta-propeller structure (2). The alpha V subunit contains domains termed thigh, calf, and calf-2 with a divalent cation-binding site found at a position equivalent to the “knee”. The 962 aa human alpha V ECD (4), which is cleaved at aa 890 but remains associated, shares 92-95% aa sequence identity with mouse and bovine alpha V, while the 685 aa human beta 6 ECD (5) shares 90-93% aa sequence identity with mouse, rat, bovine, ovine, and porcine beta 6. Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. The beta 6 C-terminal 11 amino acid (aa) cytoplasmic sequence transduces a signal, enhancing proliferation and inducing MMP-9 expression (6). Either “inside-out” signaling or Mg2+ or Mn2+ binding unfolds and activates the integrin (1). Active alpha V beta 6 binds matrix proteins fibronectin and tenascin C (2). It also binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes (7). This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1 (8, 9). Deletion of beta 6 ablates tonic inhibition of alveolar macrophages by TGF-beta, inhibits intestinal regulatory T cell production, and predisposes mice to inflammatory reactions in the skin, lungs, and intestines where irritations and microbial challenges are frequent (10-12). High alpha V beta 6 expression in carcinomas may contribute to progression through its effects on TGF-beta and MMP activity (3). The foot-and-mouth disease virus and several other viruses can use alpha V beta 6 as a receptor, and soluble alpha V beta 6 may block virus infectivity in vitro (13, 14).

References

  1. Hynes, R.O. (2002) Cell 110:673.
  2. Sheppard, D. (2004) Curr. Opin. Cell Biol. 16:552.
  3. Bandyopadhyay, A. and S. Raghavan (2009) Curr. Drug Targets 10:645.
  4. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14080.
  5. Sheppard, D. et al. (1990) J. Biol. Chem. 265:11502.
  6. Dixit, R.B. et al. (1996) J. Biol. Chem. 271:25976.
  7. Munger, J.S. et al. (1999) Cell 96:319.
  8. Fontana, L. et al. (2005) FASEB J. 19:1798.
  9. Jenkins, R.G. et al. (2006) J. Clin. Invest. 116:1606.
  10. Huang, X.Z. et al. (1996) J. Cell Biol. 133:921.
  11. Morris, D.G. et al. (2003) Nature 422:169.
  12. Chen, X. et al. (2011) J. Leukoc. Biol. 90:751.
  13. Berryman, S. et al. (2005) J. Virol. 79:8519.
  14. Heikkila, O. et al. (2009) J. Gen. Virol. 90:197.

Alternate Names

CD51, integrin subunit alpha V, ITGAV, MSK8, VNRA, VTNR

Entrez Gene IDs

3685 (Human)

Gene Symbol

ITGAV

Additional Integrin alpha V beta 6 Products

Product Documents for Recombinant Human Integrin alpha V beta 6 Fc Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Integrin alpha V beta 6 Fc Protein, CF

For research use only

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