Recombinant Human Intelectin-1/Omentin Protein, CF

Catalog # Availability Size / Price Qty
9137-IN-050

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Recombinant Human Intelectin-1/Omentin Protein Bioactivity
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Recombinant Human Intelectin-1/Omentin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Streptococcus Pneumococcal Polysaccharide Type 70 is coated at 2 µg/mL (100 μL/well), Recombinant Human Intelectin-1/Omentin binds with a typical ED50 of 3-15 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Intelectin-1/Omentin protein
Trp17-Arg313
Accession #
N-terminal Sequence
Analysis
Trp17 & Thr19
Structure / Form
Disulfide-linked homotrimer
Predicted Molecular Mass
33 kDa
SDS-PAGE
36-42 kDa, reducing conditions

Product Datasheets

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9137-IN

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9137-IN

Formulation Lyophilized from a 0.2 μm filtered solution in Tris.
Reconstitution Reconstitute at 500 μg/mL in Water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human Intelectin-1/Omentin Protein Bioactivity View Larger

When Streptococcus Pneumococcal Polysaccharide Type 70 is coated at 2 µg/mL (100 μL/well), Recombinant Human Intelectin-1/Omentin binds (Catalog # 9137-IN) with a typical ED50 of 3-15 μg/mL.

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Background: Intelectin-1/Omentin

Intelectin-1, also known as Omentin, HL-1, and Lactoferrin Receptor, is a multifunctional protein that plays a protective role in the response to cardiovascular injury (1). Mature human Intelectin-1 contains one fibrinogen C-terminal domain (2-4) and shares 82% and 80% aa sequence identity with mouse and rat Intelectin-1, respectively. Intelectin-1 is expressed by vascular smooth muscle and endothelial cells (3, 5, 6), particularly in the heart, small intestine, colon, thymus, ovary, testis, and omental fat (2, 3, 6) as well as by intestinal Paneth cells and airway and intestinal goblet cells (7-9). It is presented as a 35-40 kDa GPI-anchored glycoprotein that forms disulfide-linked homotrimers (2, 4) and can be released into the circulation and bronchiolar lavage fluid (6, 8). It binds to microbial galactofuranosyl carbohydrates (2, 11) and additionally functions as a receptor for the iron-transporting protein Lactoferrin (4). Following cardiovascular injury, Intelectin-1 limits fibrosis, cardiac hypertrophy, and carotid artery intimal hyperplasia (12, 13) foam cell generation [5], smooth muscle cell proliferation, migration, and calcification (5, 12, 14), and it also promotes revascularization (15). In addition, Intelectin-1 inhibits osteoblast bone matrix mineralization (14), enhances
insulin-stimulated uptake by adipocytes (6), supports neural stem cell viability (16), and inhibits the invasion and metastasis of neuroblastoma cells (17).

References
  1. Jaikanth, C. et al. (2013) Exp. Clin. Endocrinol. Diabetes 121:377.
  2. Tsuji, S. et al. (2001) J. Biol. Chem. 276:23456.
  3. Lee, J.-K. et al. (2001) Glycobiology 11:65.
  4. Suzuki, Y.A. et al. (2001) Biochemistry 40:15771.
  5. Watanabe, K. et al. (2016) Cardiovasc. Res. PMID 26790473.
  6. Yang, R.-Z. et al. (2006) Am. J. Physiol. Endocrinol. Metab. 290:E1253.
  7. Komiya, T. et al. (1998) Biochem. Biophys. Res. Commun. 251:759.
  8. Kerr, S.C. et al. (2014) Am. J. Respir. Crit. Care Med. 189:1005.
  9. Washimi, K. et al. (2012) PLoS One 7:e39889.
  10. Tsuji, S. et al. (2007) Glycobiology 17:1045.
  11. Wesener, D.A. et al. (2015) Nat. Struct. Mol. Biol. 22:603.
  12. Matsuo, K. et al. (2015) J. Mol. Cell. Cardiol. 79:195.
  13. Kazama, K. et al. (2014) Am. J. Physiol. Heart Circ. Physiol. 306:H1714.
  14. Xie, H. et al. (2011) Cardiovasc. Res. 92:296.
  15. Maruyama, S. et al. (2012) J. Biol. Chem. 287:408.
  16. Zhao, L.R. et al. (2015) Mol. Med. Rep. 11:1859.
  17. Li, D. et al. (2015) Mol. Cancer 14:47.
Entrez Gene IDs
55600 (Human); 16429 (Mouse)
Alternate Names
Endothelial lectin HL-1; FLJ20022; Galactofuranose-binding lectin; hIntL; HL-1; intelectin 1 (galactofuranose binding); Intelectin1; Intelectin-1; INTLHL1; ITLN1; Itlna; ITLNITLN-1; LFR; LFRIntestinal lactoferrin receptor; Omentin; Omentin-1

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Recombinant Human Intelectin-1/Omentin Protein, CF
By Riley Cleverdon on 06/17/2022
Application: CellProlif
Reason for Rating: Protein performs well, good detailed instructions for dilution/storage. Product bottle could be improved so protein does not become trapped in the lid.