Recombinant Human ISG15 E1/UBE1L Protein, CF
Recombinant Human ISG15 E1/UBE1L Protein, CF Summary
Product Specifications
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-309
| Formulation | X mg/ml (X µM) in 50 mM Hepes, pH 8.0, 100 mM NaCl, 1 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: ISG15 Activating Enzyme/UBE1L
Interferon-stimulated Gene 15 (ISG15) Activating Enzyme (E1), also known as Ubiquitin-activating Enzyme 1-like (UBE1L), UBA7, and UBE2, is a 1012 amino acid (aa) member of the Ubiquitin-activating enzyme family with a predicted molecular weight of 111 kDa. The mouse and rat ISG15 E1/UBE1L orthologs share 80% aa sequence identity with the human protein. ISG15 E1/UBE1L catalyzes the activation of the C-terminal carboxyl group of the small Ubiquitin-like modifier ISG15. Like other E1 enzymes, ISG15 E1/UBE1L contains a conserved ATP-binding domain and an active site cysteine residue, Cys599 in humans. It is expressed in multiple tissues in response to IFN-alpha or -beta, but the covalent attachment of ISG15 to protein substrates (ISGylation) is not required for IFN-alpha or -beta signaling (1,2). In addition, ISG15 E1/UBE1L knockout mice appear to have normal embryonic development and fertility (2). However, it has been suggested that ISG15 E1/UBE1L may play a role in viral immunity as these knockout mice demonstrate increased susceptibility to influenza infection (3). ISG15 E1/UBE1L has also been shown to modulate erythroid differentiation and mediate retinoid-induced differentiation of acute promyelocytic leukemia cells (4,5). ISG15 E1/UBEL1 is downregulated in lung cancer and has been shown to downregulate Cyclin D in lung cancer cells (6,7).
- Yuan, W. & R.M. Krug (2001) EMBO J. 20:362.
- Kim, K.I. et al. (2006) Mol. Cell. Biol. 26:472.
- Lai, C. et al. (2009) J. Virol. 83:1147.
- Pitha-Rowe, I. et al. (2004) J. Biol. Chem. 279:18178.
- Maragno, A.L. et al. (2011) PLoS One 6:e26068.
- Pitha-Rowe, I. et al. (2004) Cancer Res. 64:8109.
- Kok, K. et al. (1993) Proc. Natl. Acad. Sci. USA 90:6071.
Citation for Recombinant Human ISG15 E1/UBE1L Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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ERalpha phosphorylation at Y537 by Src triggers E6-AP-ERalpha binding, ERalpha ubiquitylation, promoter occupancy, and target gene expression.
Authors: Sun J, Zhou W, Kaliappan K, Nawaz Z, Slingerland J
Mol Endocrinol, 2012-08-03;26(9):1567-77.
Species: Human
Sample Types: Protein
Applications: Bioassay
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