Recombinant Human ISM1 Protein, CF

R&D Systems | Catalog # 9845-IS

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human ISM1 Protein (9845-IS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

B1AKI9

Applications

Bioactivity
View all Isthmin 1/ISM1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Isthmin 1/ISM1 protein
Ser27-Tyr464, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser27

Predicted Molecular Mass

50 kDa

SDS-PAGE

62-71 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9845-IS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Isthmin 1/ISM1

Isthmin 1 (ISM1) is an adhesion antiangiogenic molecule found in skin, muscosal tissues and immuneregulatory cells (1). The secreted human ISM1 consists of a 45 amino acid (aa) TSR domain, and a 164 aa AMOP domain. Within these domains, human ISM1 shares 92% and 93% aa sequence identity with mouse and rat ISM1, respectively (2). ISM1 shares various roles in developing embryos, from craniofacial patterning (3) to development of the lung tissues as well as tissue homeostasis in adults (4). In the vascular system, ISM1 has been described as an angiogenesis inhibitor. It inhibits angiogenesis in vitro and in vivo, as well as during tumor progression (5). A dual function of ISM1 has also been described where it exerts pro-survival effect on endothelial cells when immobilized, whereas soluble ISM1 induced endothelial cell apoptosis (6). In mouse, ISM1 is mainly expressed in the epithelium of bronchi and alveoli of the lung as well as in the cerebral cortex and hippocampus of the brain, sugggesting the relevance of this protein for the development and homeostasis of these organs (4). Our in-house bioassay has showed that ISM1 can support cortical neuron neurite outgrowth.

References

  1. Valle-Rios, R. et al. (2014). J. Interf. Cytok. Res. 34:795.
  2. Valle-Rios, R. et al. (2014) J. Interferon Cytokine Res. 34:795.
  3. Lansdon, L. et al. (2018). Genetics. 208:283.
  4. Osório, L. et al. (2014). Cell Cycle. 13:1571.
  5. Chen, M. et al. (2014) Cell Death and Differetiation. 21:797.
  6. Zhang, Y. et al. (2011) Cell Death. Dis. 2:e153.

Long Name

Isthmin 1

Alternate Names

C20orf82, ISM, ISM-1, ISM1, Isthmin, Isthmin-1

Entrez Gene IDs

140862 (Human); 319909 (Mouse); 311760 (Rat)

Gene Symbol

ISM1

UniProt

B1AKI9

Additional Isthmin 1/ISM1 Products

Product Documents for Recombinant Human ISM1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human ISM1 Protein, CF

For research use only

Related Research Areas

Integrins and Associated Molecules

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