Recombinant Human LIFR alpha Protein, CF

Newer Version Available: 7487-LR
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An Improved rh LIF Ra is Available! The new protein has ~3 fold better activity and enhanced purity!
Catalog # Availability Size / Price Qty
249-LR-050/CF
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Recombinant Human LIFR alpha Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit LIF-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 3-10 µg/mL in the presence of 0.3 ng/mL of recombinant human LIF.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human LIF R alpha protein
Gln45-Ser833
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln45 predicted
Predicted Molecular Mass
89 kDa
SDS-PAGE
100-110 kDa, reducing conditions

Product Datasheets

249-LR/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

249-LR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: LIFR alpha

The activities of the pleiotropic cytokine LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex. The gp130 subunit was first described as the signal transducing subunit of the high-affinity IL-6 receptor complex. Besides LIF, the high-affinity heterodimeric LIF receptor complex has been shown to mediate the activities of oncostatin M (OSM), cardiotrophin-1 and ciliary neurotrophic factor (CNTF).

Human LIF R alpha  cDNA encodes a 1097 amino acid (aa) residue precursor type I membrane protein with a 44 aa residue signal peptide, a 789 aa residue extracellular domain, a 26 aa residue transmembrane domain, and a 238 aa residue cytoplasmic domain. LIF R alpha is a member of the cytokine receptor family and has extensive homology to gp130. The extracellular domain of LIF R alpha  has two cytokine receptor domains and three fibronectin type III repeats. In mouse, mRNAs encoding a soluble LIF R alpha and lacking transmembrane and intracellular domains, have been isolated. Soluble LIF R alpha has been shown to bind LIF and has LIF antagonistic activity.

References
  1. Bazan, J.F. (1990) Proc. Natl. Acad. Sci. USA 87:6934.
  2. Gearing, D.P. (1994) Guidebook to Cytokines and Their Receptors, Academic Press, p130.
  3. Pennica D. et al. (1995) J. Biol. Chem. 270:10915.
Long Name
Leukemia Inhibitory Factor Receptor alpha
Entrez Gene IDs
3977 (Human); 16880 (Mouse); 81680 (Rat); 102131906 (Cynomolgus Monkey)
Alternate Names
CD118 antigen; CD118; FLJ98106; FLJ99923; leukemia inhibitory factor receptor alpha; leukemia inhibitory factor receptor; LIF R alpha; LIF R beta; LIF receptor; LIFR alpha; LIFR; LIF-R; LIFRa; SJS2; STWS; SWS

Citations for Recombinant Human LIFR alpha Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor.
    Authors: Chollangi, Srinivas, Mather, Timothy, Rodgers, Karla K, Ash, John D
    J Biol Chem, 2012;287(39):32848-59.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)
  2. Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells.
    Authors: Erices A, Conget P, Rojas C, Minguell JJ
    Exp. Cell Res., 2002;280(1):24-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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