Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF Summary
Accession # Q14108
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.|
|Reconstitution||Reconstitute at 200 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and as CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1-3). SR-B/CD36 family members are type III integral membrane proteins with an N- as well as a C-terminal cytoplasmic tail, and a large extracellular (or lumenal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is widely expressed on all tissues and cell types so far examined. It is also expressed on the surface of activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII-thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation (1). Overexpression of LIMPII causes an enlargement of early endosomes and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5).
- Crombie, R. and R. Silverstein (1998) J. Biol. Chem. 273:4855.
- Febbraio, M. et al. (2001) J. Clin. Invest. 108:785.
- Eskelinen, E-L. et al. (2003) Trends in Cell Biology 13:137.
- Kuronita, T. et al. (2002) J. Cell Sci. 115:4117.
- Gamp, A-C. et al. (2003) Human Molecular Genetics 12:631.
Citations for Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Single Neutralizing Monoclonal Antibodies Targeting the VP1 GH Loop of Enterovirus 71 Inhibit both Virus Attachment and Internalization during Viral Entry.
Authors: Ku Z, Ye X, Shi J, Wang X, Liu Q, Huang Z
J Virol, 2015;89(23):12084-95.
Sample Types: Complex Sample Type
Human SCARB2-dependent infection by coxsackievirus A7, A14, and A16 and enterovirus 71.
Authors: Yamayoshi S, Iizuka S, Yamashita T
J. Virol., 2012;86(10):5686-96.
Sample Types: Virus
Identification of a Human SCARB2 Region That Is Important for Enterovirus 71 Binding and Infection.
Authors: Yamayoshi S, Koike S,
J. Virol., 2011;85(10):4937-46.
Sample Types: Protein
Scavenger receptor B2 is a cellular receptor for enterovirus 71.
Authors: Yamayoshi S, Yamashita Y, Li J, Hanagata N, Minowa T, Takemura T, Koike S
Nat. Med., 2009;15(7):798-801.
Sample Types: Peptide
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