Recombinant Human LRIG2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1941-LR-050
Recombinant Human LRIG2 Fc Chimera Protein, CF Binding Activity
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Recombinant Human LRIG2 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LRIG2 Fc Chimera is immobilized at 4 µg/mL (100 µL/well), the concentration of Recombinant Human PDGF R beta Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR) that produces 50% of the optimal binding response is 1.5-9 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human LRIG2 protein
Human LRIG-2
(Gly41-Thr805)
Accession # O94898
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Gly41
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
112 kDa
SDS-PAGE
125-145 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1941-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human LRIG2 Fc Chimera (Catalog # 1941-LR) is coated at 4 μg/mL, 100 μL/well, Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR) binds with an ED50 of 1.5-9 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human LRIG2 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 125-145 kDa and 250-290 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRIG2

LRIG2 (leucine-rich repeats and Ig-like domains-2), also known as LIG-2, is a type I transmembrane glycoprotein member of the mammalian LRIG glycoprotein family (1). This family contains three members who share 45-50% amino acid (aa) identity (1). Human LRIG2 is synthesized as large precursor molecule, containing a signal sequence, a 767 amino acid (aa) extracellular domain (ECD), a single transmembrane sequence, and a 237 aa intracellular region. In the ECD, all LRIG family members contain at least fifteen LRRs, accompanied by two flanking cysteine-rich regions, and three C2-type Ig-like domains in their extracellular domains (ECD) (1-3). The ECD of human LRIG2 shares 94% and 93% aa sequence identity with mouse and rat, respectively. LRIG2 might have a function different from that of LRIG1, and possibly contribute to the etiology of oligodendroglioma (4). It was previously demonstrated that LRIG2 positively regulates epidermal growth factor receptor (EGFR) signaling, the most common aberrant receptor tyrosine kinase (RTK) signaling in glioblastoma multiforme (GBM), which promotes GBM growth. LRIG2 has the ability to physically interact with PDGFR beta, promoting the total expression and the activation of PDGF R beta, and enhancing its downstream signaling pathways of Akt and STAT3 and the effectors of key regulators of cell cycle progression, resulting in increased GBM cell proliferation (5).

References
  1. Guo, D. et al. (2004) Genomics 84:157.
  2. Holmlund, C. et al. (2004) Gene 332:35.
  3. Wang, B. et al. (2009) Cancer Biol Ther. 8:1018.
  4. Holmlund C et al. (2009) Neuropathology. 29:242.
  5. Qungen Xiao et al. (2018) Int J Oncol. 53:1069.
Long Name
Leucine-rich Repeats and Immunoglobulin-like Domains 2
Entrez Gene IDs
9860 (Human); 269473 (Mouse); 310753 (Rat)
Alternate Names
KIAA0806; leucine-rich repeats and immunoglobulin-like domains 2; leucine-rich repeats and immunoglobulin-like domains protein 2; LIG2; LRIG2; UFS2

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