Recombinant Human LRIG2 Fc Chimera Protein, CF

R&D Systems | Catalog # 1941-LR

R&D Systems
Discontinued Product
1941-LR has been discontinued. View all LRIG2 products.

Key Product Details

Source

CHO

Accession Number

O94898

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all LRIG2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human LRIG2 protein
Human LRIG-2
(Gly41-Thr805)
Accession # O94898		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly41

Predicted Molecular Mass

112 kDa

SDS-PAGE

125-145 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LRIG2 Fc Chimera is immobilized at 4 µg/mL (100 µL/well), the concentration of Recombinant Human PDGF R beta Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR) that produces 50% of the optimal binding response is 1.5-9 μg/mL.

Scientific Data Images for Recombinant Human LRIG2 Fc Chimera Protein, CF

Recombinant Human LRIG2 Fc Chimera Protein Binding Activity

Recombinant Human LRIG2 Fc Chimera Protein Binding Activity

When Recombinant Human LRIG2 Fc Chimera (Catalog # 1941-LR) is coated at 4 µg/mL, 100 µL/well, Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR) binds with an ED50 of 1.5-9 µg/mL.
Recombinant Human LRIG2 Fc Chimera Protein SDS-PAGE

Recombinant Human LRIG2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human LRIG2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 125-145 kDa and 250-290 kDa, respectively.

Formulation, Preparation, and Storage

1941-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRIG2

LRIG2 (leucine-rich repeats and Ig-like domains-2), also known as LIG-2, is a type I transmembrane glycoprotein member of the mammalian LRIG glycoprotein family (1). This family contains three members who share 45-50% amino acid (aa) identity (1). Human LRIG2 is synthesized as large precursor molecule, containing a signal sequence, a 767 amino acid (aa) extracellular domain (ECD), a single transmembrane sequence, and a 237 aa intracellular region. In the ECD, all LRIG family members contain at least fifteen LRRs, accompanied by two flanking cysteine-rich regions, and three C2-type Ig-like domains in their extracellular domains (ECD) (1-3). The ECD of human LRIG2 shares 94% and 93% aa sequence identity with mouse and rat, respectively. LRIG2 might have a function different from that of LRIG1, and possibly contribute to the etiology of oligodendroglioma (4). It was previously demonstrated that LRIG2 positively regulates epidermal growth factor receptor (EGFR) signaling, the most common aberrant receptor tyrosine kinase (RTK) signaling in glioblastoma multiforme (GBM), which promotes GBM growth. LRIG2 has the ability to physically interact with PDGFR beta, promoting the total expression and the activation of PDGF R beta, and enhancing its downstream signaling pathways of Akt and STAT3 and the effectors of key regulators of cell cycle progression, resulting in increased GBM cell proliferation (5).

References

  1. Guo, D. et al. (2004) Genomics 84:157.
  2. Holmlund, C. et al. (2004) Gene 332:35.
  3. Wang, B. et al. (2009) Cancer Biol Ther. 8:1018.
  4. Holmlund C et al. (2009) Neuropathology. 29:242.
  5. Qungen Xiao et al. (2018) Int J Oncol. 53:1069.

Long Name

Leucine-rich Repeats and Immunoglobulin-like Domains 2

Alternate Names

KIAA0806, LIG2, UFS2

Entrez Gene IDs

9860 (Human); 269473 (Mouse); 310753 (Rat)

Gene Symbol

LRIG2

UniProt

O94898

Additional LRIG2 Products

Product Documents for Recombinant Human LRIG2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human LRIG2 Fc Chimera Protein, CF

For research use only

Related Research Areas

EGF Family

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