Recombinant Human LRRC4 Fc Chimera Protein, CF

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Recombinant Human LRRC4 Fc Chimera Protein Bioactivity
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Recombinant Human LRRC4 Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Netrin‑G2a Fc Chimera (Catalog # 2744-NG) is coated at 1 µg/mL, Recombinant Human LRRC4 Fc Chimera (Catalog # 9375-LR) binds with an ED50 = 2-12 ng/mL.
Human embryonic kidney cell, HEK293-derived human LRRC4 protein
Human LRRC4
Accession # Q9HBW1
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
81 kDa
91-127 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human LRRC4 Fc Chimera Protein Bioactivity View Larger

When Recombinant Mouse Netrin‑G2a Fc Chimera (Catalog # 2744-NG) is coated at 1 µg/mL, Recombinant Human LRRC4 Fc Chimera (Catalog # 9375-LR) binds with an ED50 = 2-12 ng/mL.

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Reconstitution Calculator

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Background: LRRC4

LRRC4 (Leucine rich repeat/LRR-containing protein 4), also called NGL-2 (netrin-G ligand-2) or NAG14 (nasopharyngeal carcinoma-associated gene 14), is a 55 kDa (predicted) type I transmembrane protein that is a member of the NGL family of synaptic LRR adhesion molecules (1, 2). Human LRRC4 cDNA encodes 653 amino acids (aa) that include a 38 aa signal sequence, a 489 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 105 aa cytoplasmic domain. The ECD contains nine LRRs (aa 74-288), a C2 type Ig like domain (aa 354-440), and a Thr-rich segment (aa 455-526). Within the ECD, human LRRC4 shares 98% aa identity with mouse and rat, 99% aa identity with canine and bovine, and 99.6% aa identity with equine LRRC4. It also shares 54-55% aa identity with family members LRRC4C/NGL-1 and LRRC4B/NGL-3, but each recognizes different ligands (1). LRRC4 is predominantly expressed in the brain on neurons and astrocytes as a ligand for netrin-G2 in post-synaptic membranes (2-4). It is proposed to regulate the formation of excitatory synapses via recruitment of PSD-95 to the cytoplasmic domain after aggregation of LRRC4 at the surface (3, 5). It suppresses proliferation by down-regulating cell signaling pathways, resulting in altered expression of cell cycle regulating proteins and delay at the late G1 phase (1, 2, 6-8). It is thus considered a tumor suppressor protein and is often down-regulated in brain tumors, particularly gliomas (1, 2, 6). Forced expression of LRRC4 in tumor cells slows proliferation and promotes differentiation (1, 4, 9).

  1. Woo, J. et al. (2009) Mol. Cell. Neurosci. 42:1.
  2. Zhang, Q. et al. (2005) FEBS Lett. 579:3674.
  3. Kim, S. et al. (2006) Nat. Neurosci. 9:1294.
  4. Wu, M. et al. (2007) Acta Biochim Biophys Sin (Shanghai) 39:731.
  5. Nishimura-Akiyoshi, S. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14801.
  6. Wu, M. et al. (2006) Mol. Biol. Cell 17:3534.
  7. Wu, M. et al. (2008) J. Cell. Biochem. 103:245.
  8. Wu, M. et al. (2008) J. Cell. Physiol. 214:65.
  9. Zhang, W. et al. (2008) Genes Brain Behav. 7:385.
Long Name
Leucine-rich Repeat Containing 4
Entrez Gene IDs
64101 (Human); 192198 (Mouse); 641521 (Rat)
Alternate Names
BAG; Brain Tumor Associated Protein LRRC4; Brain Tumor-Associated Protein BAG; Leucine Rich Repeat Containing 4; Leucine-Rich Repeat-Containing 4; Leucine-Rich Repeat-Containing Protein 4; LRRC4; NAG14; Nasopharyngeal Carcinoma-Associated Gene 14 Protein; Netrin-G2 Ligand; NGL-2


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